Description |
1 online resource (xi, 369 pages) : illustrations (some color) |
Series |
Methods in molecular biology, 1940-6029 ; Volume No. 1179 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1179. 1064-3745
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Contents |
PART I. POINT MUTAGENESIS -- 1. Error-Prone PCR and Effective Generation of Gene Variant Libraries for Directed Evolution / Janine N. Copp, Paulina Hanson-Manful, David F. Ackerley, and Wayne M. Patrick -- 2. Error-Prone Rolling Circle Amplification Greatly Simplifies Random Mutagenesis / Ryota Fujii, Motomitsu Kitaoka, and Kiyoshi Hayashi -- 3. Random Mutagenesis by Error-Prone Pol Plasmid Replication in Escherichia coli / David L. Alexander, Joshua Lilly, Jaime Hernandez, Jillian Romsdahl, Christopher J. Troll, and Manel Camps -- 4. The Sequence Saturation Mutagenesis (SeSaM) Method / Anna Joëlle Ruff, Tsvetan Kardashliev, Alexander Dennig, and Ulrich Schwaneberg -- 5. Generation of Effective Libraries by Neutral Drift / Miriam Kaltenbach and Nobuhiko Tokuriki -- 6. Site-Saturation Mutagenesis by Overlap Extension PCR / Elsie M. Williams, Janine N. Copp, and David F. Ackerley -- 7. Iterative Saturation Mutagenesis: A Powerful Approach to Engineer Proteins by Systematically Simulating Darwinian Evolution / Carlos G. Acevedo-Rocha, Sabrina Hoebenreich, and Manfred T. Reetz -- 8. Generating Targeted Libraries by the Combinatorial Incorporation of Synthetic Oligonucleotides During Gene Shuffling (ISOR) / Liat Rockah-Shmuel, Dan S. Tawfik, and Moshe Goldsmith -- 9. OmniChange: Simultaneous Site Saturation of Up to Five Codons / Alexander Dennig, Jan Marienhagen, Anna Joëlle Ruff, and Ulrich Schwaneberg -- 10. Random Insertional-Deletional Strand Exchange Mutagenesis (RAISE): A Simple Method for Generating Random Insertion and Deletion Mutations / Rota Fujii, Motomitsu Kitaoka, and Kiyoshi Hayashi -- 11. Transposon-Based Approaches for Generating Novel Molecular Diversity During Directed Evolution / D. Dafydd Jones, James A.J. Arpino, Amy J. Baldwin, and Matthew C. Edmundson |
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PART II. RECOMBINATORIAL METHODS -- 12. Restriction Enzyme-Mediated DNA Family Shuffling / James B.Y.H. Behrendorff, Wayne A. Johnston, and Elizabeth M.J. Gillam -- 13. Assembly of Designed Oligonucleotides: A Useful Tool in Synthetic Biology for Creating High-Quality Combinatorial DNA Libraries / Carlos G. Acevedo-Rocha and Manfred T. Reetz -- 14. One-Pot Simple Methodology for Cassette Randomization and Recombination for Focused Directed Evolution (OSCARR) / Aurelio Hidalgo, Anna Schließmann, and Uwe T. Bornscheuer -- 15. USER Friendly DNA Recombination (USERec): Gene Library Construction Requiring Minimal Sequence Homology / Benoit Villiers and Florian Hollfelder -- 16. ITCHY: Incremental Truncation for the Creation of Hybrid Enzymes / Wayne M. Patrick and Monica L. Gerth -- 17. Generating Random Circular Permutation Libraries / Stefan Lutz, Ashley B. Daugherty, Ying Yu, and Zhen Qian |
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PART III. STRUCTURE-GUIDED METHODS AND COMPUTATIONAL TOOLS FOR THE DESIGN AND ANALYSIS OF MUTANT LIBRARIES -- 18. Probabilistic Methods in Directed Evolution: Library Size, Mutation Rate, and Diversity / Yuval Nov -- 19. The Mutagenesis Assistant Program / Rajni Verma, Tuck Seng Wong, Ulrich Schwaneberg, and Danilo Roccatano -- 20. Computational Tools for Designing Smart Libraries / Eva Sebestova, Jaroslav Bendl, Jan Brezovsky, and Jiri Damborsky -- 21. Computational Tools for Directed Evolution: A Comparison of Prospective and Retrospective Strategies / Julian Zaugg, Yosephine Gumulya, Elizabeth M.J. Gillam, and Mikael Bodén -- 22. Designing Libraries of Chimeric Proteins Using SCHEMA Recombination and RASPP / Matthew A. Smith and Frances H. Arnold -- 23. Noncontiguous SCHEMA Protein Recombination / Matthew A. Smith and Frances H. Arnold -- 24. Engineering Proteins by Reconstructing Evolutionary Adaptive Paths / Vanessa E. Cox and Eric A. Gaucher |
Summary |
Directed Evolution Library Creation: Methods and Protocols, Second Edition presents user-friendly protocols for both proven strategies and cutting-edge approaches for the creation of mutant gene libraries for directed evolution |
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As well as experimental methods, information on current computational approaches is provided in a user-friendly format that will allow researchers to make informed choices without needing to comprehend the full technical details of each algorithm |
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Directed evolution has become a fundamental approach for engineering proteins to enhance activity and explore structure-function relationships, and has supported the rapid development of the field of synthetic biology over the last decade |
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Divided into three convenient sections, topics include point mutagenesis strategies, recombinatorial methods wherein genetic diversity is sourced from multiple parental genes that are combined via either homology-dependent or -independent techniques, and a variety of computational methods to guide the design and analysis of mutant libraries |
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Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls |
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Authoritative and easily accessible, Directed Evolution Library Creation: Methods and Protocols, Second Edition will serve as a reliable manual for both novice and experienced protein engineers and synthetic biologists and will enable further technical innovation and the exploitation of directed evolution for a deeper understanding of protein design and function |
Analysis |
evolutie |
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evolution |
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biomedische wetenschappen |
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biomedicine |
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genetische modificatie |
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genetic engineering |
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humane genetica |
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human genetics |
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Medicine (General) |
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Geneeskunde (algemeen) |
Bibliography |
Includes bibliographical references and index |
Subject |
Gene libraries -- Laboratory manuals
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Genetic engineering -- Laboratory manuals
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Molecular evolution -- Laboratory manuals
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Microbial mutation -- Laboratory manuals
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Combinatorial chemistry -- Laboratory manuals
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Genetic recombination -- Laboratory manuals
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Gene libraries.
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Directed Molecular Evolution
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Combinatorial Chemistry Techniques
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Gene Library
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Gene libraries
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Genetic engineering
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Molecular evolution
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Microbial mutation
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Combinatorial chemistry
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Genetic recombination
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Biology -- Laboratory manuals
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Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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Form |
Electronic book
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Author |
Gillam, Elizabeth M., editor
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Copp, Janine N., editor
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Ackerley, David F., editor
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LC no. |
2014943737 |
ISBN |
9781493910533 |
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1493910531 |
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