| Description |
1 online resource (xi, 358 pages, 8 unnumbered pages of plates) : illustrations (some color) |
| Series |
Methods in molecular biology ; 467 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; v. 467.
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Springer protocols (Series)
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| Contents |
Angiogenic signalling pathways / Zubair Ahmed and Roy Bicknell -- Imaging angiogenesis / Natalie Charnley, Stephanie Donaldson, and Pat Price -- Assessing tumor angiogenesis in histological samples / Stephen B. Fox -- Immunohistochemical methods for measuring tissue lymphangiogenesis / Steven Clasper and David G. Jackson -- Vascular endothelial cells from human micro- and macrovessels : isolation, characterisation, and culture / Peter W. Hewitt -- Lymphatic endothelial cells : establishment of primaries and characterization of established lines / Riccardo E. Nisato, Raphaele Buser, and Michael S. Pepper -- Isolation, culture, and characterisation of vascular smooth muscle cells / Adrian T. Churchman and Richard C.M. Siow -- Circulating endothelial progenitor cells / Michael C. Schmid and Judith A. Varner -- In vitro assays for endothelial cell functions related to angiogenesis : proliferation, motility, tubular differentiation, and proteolysis / Suzanne A. Eccles [and others] -- Tube formation : an invitro matrigel angiiogenesis assay / M. Lourdes Ponce -- Three-dimensional in vitro angiogenesis in the rat aortic ring model / David C. West and Mike F. Burbridge -- Static and dynamic assays of cell adhesion relevant to the vasculature / Lynn M. Butler, Helen M. McGettrick, and Gerard B. Nash -- Genetic manipulation of corneal endothelial cells : transfection and viral transduction / Eckart Bertelmann -- Creation of human skin equivalents for the in vitro study of angiogenesis in wound healing / Ira M. Herman and Alice Leung -- Measurement of angiogenic phenotype by use of two-dimensional mesenteric angiogenesis assay / Andrew V. Benest and David O. Bates -- Quantitative estimation of tissue blood flow rate / Gillian M. Tozer, Viven E. Prise, and Vincent J. Cunningham -- In vivo matrigel migration and angiogenesis assay / Katherine M. Malinda -- The sponge implant model of angiogenesis / Silvia Passos Andrade and Mônica Alves Neves Diniz Ferreira -- The dorsal skinfold chamber : studying angiogenesis by intravital microscopy / Axel Sckell and Michael Leunig -- The Cornell pocket assay / Marina Ziche and Lucia Morbidelli -- Use of the hollow fibre assay for studies of tumor neovasculature / Steven D. Shnyder -- The cranial bone window model : studying angiogenesis of primary and secondary bone tumors by intravital microscopy / Axel Sckell and Frank M. Klenke |
| Summary |
As experimentation and clinical trials with first generation anti-angiogenic agents have yielded results and our understanding of the biology and physiology of blood and lymphatic vessels has increased, a new angiogenesis volume swiftly became a necessity. Angiogenesis Protocols, Second Edition remains true to its original vision of providing a single source for angiogenesis researchers, irrespective of levels of resource and expertise, by collecting a range of methods for cell isolation and assessing angiogenesis in vivo or in vitro. This information, however, is expanded to include chapters on circulating endothelial progenitor cells, angiogenic signalling pathways, imaging of angiogenesis, and measurement of tissue blood flow. Written in the Methods in Molecular Biology"!series format, the chapters provide step-by-step laboratory protocols, lists of necessary materials and reagents, and a Notes section, which details tips on troubleshooting and avoiding known pitfalls. Extensive and cutting-edge, Angiogenesis Protocols, Second Edition is not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Neovascularization -- Laboratory manuals
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Blood-vessels -- Growth -- Molecular aspects -- Laboratory manuals
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Neovascularization.
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Angiogenesis Inducing Agents -- analysis
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Biological Assay -- methods
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Neovascularization, Pathologic
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Neovascularization
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Martin, Stewart, 1962-
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Murray, J. Clifford
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| ISBN |
9781588299079 |
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1588299074 |
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9781597452410 |
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1597452416 |
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9781607610700 |
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1607610701 |
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9781627038010 |
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1627038019 |
|
