| Description |
1 online resource (242 pages) |
| Contents |
2.8 MRV 1-like Cell Attachment Protein of GCRV-II Species -- 2.9 Conclusions and Future Considerations -- References -- Chapter 3: The Aquareovirus Particle Structure and Protein Functions -- 3.1 Introduction -- 3.2 The Aquareovirus Particle and Its Structural Protein Localization -- 3.2.1 Similarities and Differences between the Aquareovirus, Orthoreovirus, and Other Viruses in the Family Reoviridae -- 3.2.2 Comparison of Structural Proteins in the Aquareoviruses and Orthoreoviruses -- 3.3 Cryo-EM Analysis of the Aquareovirus/GCRV Virions |
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Intro -- Preface -- Contents -- Contributors -- Chapter 1: Aquareovirus: An Overview -- 1.1 Introduction -- 1.2 Origin of AqRV -- 1.3 Characteristic Features of AqRV -- 1.4 Taxonomy and Classification of the Genus AqRV -- 1.5 Geographic and Seasonal Distribution -- 1.6 Host Range and Viral Proliferation -- 1.7 Antigenic and Serological Characterization of AqRV -- 1.8 Representative and Pathogenic AqRV Strains -- 1.9 Conclusions and Future Considerations -- References -- Chapter 2: Molecular Biology of Aquareoviruses -- 2.1 Introduction -- 2.2 Particle-Based Properties |
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2.2.1 Virus Proliferation and Sample Preparation -- 2.2.2 Different Particle Components Extracted from Purified Virus Preparations -- 2.2.2.1 Intact Virions and Empty Particles -- 2.2.2.2 SVP and Virus Core -- 2.2.3 The Virus-Antibody Immunocompound of GCRV -- 2.2.4 Recombinant GCRV Particles -- 2.2.5 Surface Labeling of AqRV Particles -- 2.2.6 Infectivity Assays of Purified or Modified Virions and SVPs -- 2.3 AqRV Structural Protein Profile -- 2.3.1 Identification of AqRV Particle Proteins -- 2.3.2 Various Particle Forms and Related Protein Profiles |
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2.4 In Vitro Endogenous Transcription Assays of GCRV Particles -- 2.4.1 GCRV Nucleic Acid Release -- 2.4.2 GCRV Reaction Core -- 2.4.3 In Vitro Transcription Assays -- 2.5 The AqRV Genome -- 2.5.1 Nomenclature and Electrophoresis Profile of the AqRV dsRNA Genome -- 2.5.2 Genome Sequence-Based Features of AqRVs -- 2.5.3 Terminal Non-coding Regions -- 2.5.4 Protein-Coding Assignments and Nomenclature -- 2.5.4.1 SBRV Gene-Protein-Coding Assignment -- 2.5.4.2 GCRV Gene-Protein-Coding Assignment -- 2.5.5 In Vitro and In Vivo Protein Expression Analyses -- 2.6 AqRV Morphogenesis |
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2.6.1 TEM Analysis of VIB Formation in Infected Cells -- 2.6.2 NS80: A Scaffold of VIBs -- 2.6.3 Interaction of NS80 with Viral Proteins -- 2.6.4 Role of NS38 in GCRV Morphogenesis -- 2.7 GCRV S7 Genome Segment-Encoded FAST Protein and Associated Syncytium Formation -- 2.7.1 Detection of FAST Protein -- 2.7.2 Sequence-Based Features of FAST Protein -- 2.7.3 NS16 and NS22: The AqRV FAST Proteins -- 2.7.4 NS12: A Novel Membrane-Associated Protein -- 2.7.5 Role of NS31 in Virus Replication -- 2.7.6 Possible Function of GCRV NS26 in Syncytia Formation |
| Summary |
Aquareoviruses cause infection in bony fish and shellfish and thus, constitute a significant threat to aquaculture industries worldwide. Aquareoviruses, belonging to the family Reoviridae, have genomes consisting of 11 segments of double-stranded RNA contained within a core (T = 1) surrounded by a double-layered icosahedral capsid with a T = 13 symmetry in general. These viruses not only physically resemble mammalian orthoreoviruses, but also show the highest amino acid identity. More than hundred aquareoviruses have been isolated from both saline water and freshwater origins; however, literary sources elucidating aquareovirus biology are very limited. Given the increasing pace of discovery, it is imperative to make a clear, systematic filing of key aspects of aquareoviruses. Therefore, the aim of writing this book is to provide insights into the molecular mechanisms of evolution, pathogenesis, and host response in aquareovirus infection. This book offers a state-of-the-art report on recent discoveries concerning the aquareovirus genome evolution, gene encoded protein functions, and pathogenesis by comparison with its sister genus Orthoreovirus, including avian and mammalian reoviruses. It mainly focuses on advances made over the past 30 years in research on the general and molecular biology, protein structure and function, infection and replication, epidemiology and diagnosis, immunological prevention and medical treatment, and host antiviral immunity against aquareovirus infection. This book will help curious graduate students or interested researchers acquire an overall picture of aquareovirus infection and pathogenesis, as well as yield benefits in fisheries to better prevent and control diseases caused by aquareovirus infection |
| Notes |
3.3.1 Electron Cryomicrograph Records of the Purified GCRV Cores and Virions |
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Print version record |
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Online resource; title from PDF title page (SpringerLink, viewed July 28, 2021) |
| Subject |
Fishes -- Diseases.
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Shellfish -- Diseases
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Reoviruses.
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Fish Diseases
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Reoviridae
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Fishes -- Diseases
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Reoviruses
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Shellfish -- Diseases
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Malalties dels peixos.
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Virus.
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| Genre/Form |
Llibres electrònics.
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| Form |
Electronic book
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| Author |
Fang, Qin, editor
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| ISBN |
9789811619038 |
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9811619034 |
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