| Description |
1 online resource (687 pages) |
| Series |
Methods in Enzymology ; v.312 |
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Methods in enzymology.
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| Contents |
Front Cover; Sphingolipid Metabolism and Cell Signaling; Copyright Page; Table of Contents; Contributors to Volume 312; Preface; Volumes in Series; Section I: Methods for Analyzing Sphingolipids; Chapter 1. Analysis of Sphingoid Bases and Sphingoid Base 1-Phosphates by High-Performance Liquid Chromatography; Chapter 2. Enzymatic Method for Measurement of Sphingosine 1-Phosphate; Chapter 3. Ceramide Mass Analysis by Normal-Phase High-Performance Liquid Chromatography; Chapter 4. Quantitative Determination of Ceramide Using Diglyceride Kinase |
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Chapter 5. Analysis of Sphingomyelin, Glucosylceramide, Ceramide, Sphingosine, and Sphingosine 1-Phosphate by Tandem Mass SpectrometryChapter 6. Analyses of Glycosphingolipids by High-Performance Liquid Chromatography; Chapter 7. Sphingolipid Extraction and Analysis by Thin-Layer Chromatography; Chapter 8. Extraction and Analysis of Multiple Sphingolipids from a Single Sample; Chapter 9. Purification of Sphingolipid Classes by Solid-Phase Extraction with Aminopropyl and Weak Cation Exchanger Cartridges; Chapter 10. Ganglioside Analysis by High-Performance Thin-Layer Chromatography |
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Chapter 11. Purification and Analysis of GangliosidesChapter 12. Thin-Layer Chromatography Blotting Using Polyvinylidene Difluoride Membrane (Far-Eastern Blotting) and Its Applications; Chapter 13. Thin-Layer Chromatography Immunostaining; Chapter 14. Monoclonal Anti-Glycosphingolipid Antibodies; Chapter 15. Immunolocalization of Gangliosides by Light Microscopy Using Anti-Ganglioside Antibodies; Chapter 16. Cloud-Point Extraction of Gangliosides Using Nonionic Detergent C14EO6; Chapter 17. Analyses of Glycosphingolipids Using Clam, Mercenaria mercenaria, Ceramide Glycanase |
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Chapter 18. Quantitative Analyses of Binding Affinity and Specificity for Glycolipid Receptors by Surface Plasmon ResonanceChapter 19. Use of Circular Dichroism for Assigning Stereochemistry of Sphingosine and Other Long-Chain Bases; Chapter 20. Infrared Determination of Conformational Order and Phase Behavior in Ceramides and Stratum Corneum Models; Chapter 21. Use of Nuclear Magnetic Resonance Spectroscopy in Evaluation of Ganglioside Structure, Conformation, and Dynamics; Chapter 22. Fluorescence Quenching Assay of Sphingolipid/ Phospholipid Phase Separation in Model Membranes |
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Section II: Methods for Analyzing Aspects of Sphingolipid Metabolism in Intact CellsChapter 23. Synthesis of Fluorescent Substrates and Their Application to Study of Sphingolipid Metabolism in Vitro and in Intact Cells; Chapter 24. Selection of Mammalian Cell Mutants in Sphingolipid Biosynthesis; Chapter 25. Selection of Yeast Mutants in Sphingolipid Metabolism; Chapter 26. Fluorescence-Based Selection of Gene-Corrected Hematopoietic Stem and Progenitor Cells Based on Acid Sphingomyelinase Expression; Chapter 27. Mammalian Ganglioside Sialidases: Preparation and Activity Assays |
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Section III: Sphingolipid-Protein Interactions and Cellular Targets |
| Summary |
This volume contains information on analyzing sphingolipids, sphingolipid transport and trafficking, and sphingolipid-protein interactions and cellular targets. Its companion Volume 311 presents methods used in studying enzymes of sphingolipid biosynthesis and turnover, including inhibitors of some of these enzymes, genetic approaches, and organic and enzymatic syntheses of sphingolipids and analogs. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955 |
| Notes |
Description based upon print version of record |
| Subject |
Cells.
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Sphingolipids.
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Cell Communication.
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Sphingolipids -- metabolism.
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| Form |
Electronic book
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| Author |
Simon, Melvin I.
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Merrill, Jr. Alfred H
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Hannun, Yusuf A., 1955-
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| ISBN |
9780080496696 : |
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