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Book Cover
E-book

Title Lipidomics : methods and protocols / edited by Sanjoy K. Bhattacharya
Published New York, NY : Humana Press : Springer, [2017]
©2017

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Description 1 online resource (xii, 281 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; 1609
Methods in molecular biology (Clifton, N.J.) ; v. 1609. 1064-3745
Contents Lipid sample preparation for biomedical research / Ravin Sajnani and Katyayini Aribindi -- Lipid extraction techniques for stable isotope analysis and ecological assays / Kyle H. Elliott, James D. Roth, and Kevin Crook -- Isolation of lipid raft proteins from CD133+ cancer stem cells / Vineet K. Gupta and Sulagna Banerjee -- Isolation of neuronal synaptic membranes by sucrose gradient centrifugation / Blake R. Hopiavuori, Dustin R. Masser, Joseph L. Wilkerson, Richard S. Brush, Nawajes A. Mandal, Robert E. Anderson, and Willard M. Freeman -- Sample preparation and analysis for imaging mass spectrometry / Genea Edwards, Annia Mesa, Robert I. Vazquez-Padron, Jane-Marie Kowalski, and Sanjoy K. Bhattacharya -- Direct measurement of free and esterified cholesterol mass in differentiated human podocytes : a TLC and enzymatic assay-based method / Christopher E. Pedigo, Sandra M. Merscher, and Alessia Fornoni -- High-performance chromatographic separation of cerebrosides / Renaud Sicard and Ralf Landgraf -- Lipid identification by untargeted tandem mass spectrometry coupled with ultra-high-pressure liquid chromatography / Gabriel B. Gugiu -- Utility of moderate and high-resolution mass spectrometry for class-specific lipid identification and quantification / Maria del Carmen Piqueras -- Robust lipidomics workflow for mammalian cells, plasma, and tissue using liquid-chromatography high-resolution tandem mass spectrometry / Candice Z. Ulmer, Rainey E. Patterson, Jeremy P. Koelmel, Timothy J. Garrett, and Richard A. Yost -- Combined use of MALDI-TOF mass spectrometry and 31P NMR spectroscopy for analysis of phospholipids / Jenny Schröter, Yulia Popkova, Rosmarie Süss, and Jürgen Schiller -- Global monitoring of the mammalian lipidome by quantitative shotgun lipidomics / Inger Ødum Nielsen, Kenji Maeda, and Mesut Bilgin -- Bioinformatics pertinent to lipid analysis in biological samples / Justin Ma, Ulises Arbelo, Yenifer Guerra, Katyayini Aribindi, Sanjoy K. Bhattacharya, and Daniel Pelaez -- LC-MS-based lipidomics and automated identification of lipids using the LipidBlast in-silico MS/MS library / Tomas Cajka and Oliver Fiehn -- Single-step capture and targeted metabolomics of alkyl-quinolones in outer membrane vesicles of Pseudomonas aeruginosa / Pallavi Lahiri and Dipankar Ghosh -- Analysis of fatty acid and cholesterol content from detergent-resistant and detergent-free membrane microdomains / Mark E. McClellan and Michael H. Elliott -- Computational functional analysis of lipid metabolic enzymes / Carolina Bagnato, Arjen Ten Have, María B. Prados, and María V. Beligni -- Isoprenylation of monomeric GTPases in human trabecular meshwork cells / Evan B. Stubbs Jr. -- Purification and validation of lipid transfer proteins / Matti A. Kjellberg, Anders P.E. Backman, Anna Möuts, and Peter Mattjus -- Incorporation of artificial lipid-anchored proteins into cultured mammalian cells / Rania Leventis and John R. Silvius -- Sonication-based basic protocol for liposome synthesis / Roberto Mendez and Santanu Banerjee -- On electrochemical methods for determination of protein-lipid interaction / Zhiping Hu and Yanli Mao -- Angiogenesis model of cornea to understand the role of sphingosine 1-Phosphate / Joseph L. Wilkerson and Nawajes A. Mandal
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerProtocol, viewed June 29, 2017)
Subject Lipids -- Laboratory manuals
Lipids.
Lipids -- Metabolism.
Mass spectrometry.
Lipids
Lipids -- analysis
Lipids -- isolation & purification
Lipid Metabolism
Mass Spectrometry
lipid.
mass spectrometry.
Biochemistry.
Science -- Life Sciences -- Biochemistry.
Mass spectrometry
Lipids -- Metabolism
Lipids
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Bhattacharya, Sanjoy K. (Professor of ophthalmology), editor.
ISBN 9781493969968
149396996X