| Description |
1 online resource (x, 182 pages) : illustrations |
| Series |
Methods in molecular biology, 1940-6029 ; volume 1697 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1697. 1064-3745
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| Contents |
Measuring Sphingosine-1-Phosphate/Protein Interactions with the Kinetic Exclusion Assay -- An Improved Isoform-Selective Assay for Sphingosine Kinase 1 Activity -- Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells -- Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry -- Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples and Cell Lines -- A Clean-Up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule -- A Rapid Fluorescence Assay for Measuring Sphingosine-1-Phosphate Transporter Activity in Erythrocytes -- Analysis of S1P Receptor Expression by Uterine Immune Cells Using Standardized Multi-Parametric Flow Cytometry -- S1P Synergizes with Wall Shear Stress and Other Angiogenic Factors to Induce Endothelial Cell Sprouting Responses -- In Vitro Methods to Study the Modulation of Migration and Invasion by Sphingosine-1-Phosphate -- Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor -- Sphingosine-1-Phosphate (S1P) Signaling in Neural Progenitors -- Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation -- 3D Stacked Construct: A Novel Substitute for Corneal Tissue Engineering |
| Summary |
This fully updated edition explores methods involving sphingosine-1-phosphate (S1P), a bioactive lysophospholipid which has become the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells. Here, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology. As a volume in the Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols, Second Edition is another key resource for scientists working in this fast-moving and dynamic field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed December 8, 2017) |
| Subject |
Sphingosine -- Laboratory manuals
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Sphingosine.
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Sphingolipids.
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Sphingosine
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Sphingolipids
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Cellular biology (cytology)
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Science -- Life Sciences -- Biology -- Molecular Biology.
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Sphingolipids
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Sphingosine
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Pébay, Alice, editor.
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Turksen, Kursad, editor.
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| ISBN |
9781493974139 |
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1493974130 |
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1493974122 |
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9781493974122 |
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