| Description |
1 online resource (xi, 356 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 2113 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 2113. 1064-3745
|
| Summary |
This volume looks at the different spectroscopic and biophysical methods used by researchers to study the structure and folding of RNA, and to follow their interactions with proteins. The chapters in this book cover topics such as single-molecule spectroscopy of multiple RNA species; surface plasmon resonance, MS or microcalorimetry for investigating molecular interactions with RNA; FTIR, SAXS, SANS and SRCD spectroscopies to analyze RNA structure; use of fluorescent nucleotides to map RNA-binding sites on proteins surfaces or CryoEM; and much more. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls |
| Bibliography |
Includes bibliographical references and index |
| Subject |
RNA -- Laboratory manuals
|
|
RNA Folding -- physiology
|
|
Spectrum Analysis -- methods
|
|
Spectrum analysis, spectrochemistry, mass spectrometry.
|
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Medical genetics.
|
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Science -- Spectroscopy & Spectrum Analysis.
|
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Medical -- Genetics.
|
|
RNA.
|
| Genre/Form |
Laboratory manuals.
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Arluison, Véronique, editor
|
|
Wien, Frank, editor
|
| ISBN |
9781071602782 |
|
1071602780 |
|
1071602772 |
|
9781071602775 |
|
