| Description |
1 online resource (518 pages) |
| Series |
Wiley Series on Surface and Interfacial Chemistry |
| Contents |
PROTEINS IN SOLUTION AND AT INTERFACES; CONTENTS; PREFACE; CONTRIBUTORS; PART I; 1 X-RAY CRYSTALLOGRAPHY OF BIOLOGICAL MACROMOLECULES: FUNDAMENTALS AND APPLICATIONS; 1.1 INTRODUCTION; 1.2 FUNDAMENTALS OF X-RAY DIFFRACTION; 1.2.1 X-Ray Radiation and Interaction with Matter; 1.2.2 Crystals and Symmetry; 1.2.3 Diffraction by Crystals; 1.2.4 Real and Reciprocal Space; 1.2.5 Structure Factors; 1.2.6 Fourier Synthesis and Transform; 1.2.7 The Phase Problem; 1.3 THE STRUCTURE DETERMINATION PROCESS; 1.3.1 Sample Production and Conditioning; 1.3.2 Crystallization; 1.3.3 Data Collection and Processing |
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1.3.4 Structure Determination1.3.5 Electron Density Map Interpretation: Model Construction; 1.3.6 Model Refinement; 1.3.7 Validation; 1.4 STRUCTURAL ANALYSIS AND BIOLOGICAL IMPLICATIONS; 1.4.1 Structural Analysis; 1.4.2 Biological Implications; 1.5 FUTURE PROSPECTS; ACKNOWLEDGMENTS; REFERENCES; 2 NUCLEAR MAGNETIC RESONANCE METHODS FOR STUDYING SOLUBLE, FIBROUS, AND MEMBRANE-EMBEDDED PROTEINS; 2.1 INTRODUCTION AND BACKGROUND; 2.1.1 Nuclear Angular Momentum; 2.1.2 Chemical Shifts; 2.1.3 Nuclear Spin Interactions; 2.1.4 Relaxation; 2.1.5 Isotopic Labeling; 2.1.6 Samples; 2.2 STRUCTURAL DATA |
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3 SMALL-ANGLE X-RAY SCATTERING APPLIED TO PROTEINS IN SOLUTION3.1 INTRODUCTION; 3.2 SAXS THEORY; 3.2.1 General Equations; 3.2.2 Isotropy; 3.2.3 Homogeneous Scattering Particles (Two-Phase Model); 3.2.4 Model-Independent Shape Analysis: Kratky's Representation, Guinier's Law, and Porod's Invariant; 3.2.5 Methods to Calculate P(q); 3.2.6 Protein-Protein Interaction: S(q) Evaluation; 3.3 EXAMPLES; 3.3.1 Rg, p(r), Kratky's Representation and Shape Reconstruction: Analysis of Protein TcOYE; 3.3.2 Guinier's Analysis and Multipole Expansion Shape Reconstruction: The Replication Factor C Case |
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3.3.3 Partial Folded and Unfolded Protein: Analysis of the Protein Bovine Serum Albumin, BSA, Under the Influence of Denaturing Agents3.4 PROTEIN INTERACTION: BSA CASE; 3.5 PROTEIN AGGREGATION; 3.6 CONCLUSION; ACKNOWLEDGMENTS; REFERENCES; 4 ANALYZING THE SOLUTION STATE OF PROTEIN STRUCTURE, INTERACTIONS, AND LIGANDS BY SPECTROSCOPIC METHODS; 4.1 INTRODUCTION; 4.1.1 Overview; 4.1.2 Protein Secondary Structures and Motifs; 4.1.3 Structure Determination: Spectroscopic Methods; 4.2 ULTRAVIOLET-VISIBLE ABSORPTION SPECTROSCOPY; 4.2.1 Background |
| Summary |
"Proteins in solution and at interfaces are increasingly used in exciting new applications, from biomimetic materials to nanoparticle patterning. This book surveys the state-of-the-art in the field, providing scientists in diverse areas with a comprehensive and modern analysis of the techniques used in protein characterization, as well as insight into the most important applications. Topics include protein and protein aggregate structure; computational and experimental techniques for the study of protein structure, aggregation, and adsorption; proteins in non-standard conditions; and biotechnological applications"-- Provided by publisher |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Proteins -- Biotechnology
|
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Proteins -- Biotechnology
|
| Form |
Electronic book
|
| Author |
Pi?eiro,?ngel
|
| LC no. |
2012050742 |
| ISBN |
9781118523186 |
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1118523180 |
|
0470952512 |
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9780470952511 |
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9781299147874 |
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1299147879 |
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