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Title Cell-free protein expression / [edited by] Wieslaw A. Kudlicki, Federico Katzen, Robert P. Bennett
Published Austin, Tex. : Landes Bioscience, 2007

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Description 1 online resource (1 volume)
Series Biotechnology intelligence unit
Biotechnology intelligence unit (Unnumbered)
Contents The role of cell-free rabbit reticulocyte expression systems in functional proteomics / Michele Arduengo, Elaine Schenborn, and Robin Hurst -- Advances in Insect-based cell-free protein expression / Uritza von Groll [and others] -- Advantages and applications of the batch-formatted E. coli cell-free expression system / Julia E. Fletcher [and others] -- Energetics in Escherichia coli-based batch cell-Free systems / Kalavathy Sitaraman and Deb K. Chatterjee -- Disulfide bond formation in bacteria-based cell-free protein expression / Aaron R. Goerke and James R. Swartz -- The PURE system: a minimal cell-free translation system / Bei-Wen Ying, Yoshihiro Shimizu and Takuya Ueda -- Cell-free expression approaches for the production and characterization of membrane proteins / Daniel Schwarz [and others] -- Cell-free expression for protein NMR / A.J. Shaka -- Cell-free synthesis of membrane proteins for X-ray crystallography / Julia E. Fletcher [and others]
Bacterial cell-free expression systems for high-throughput protein production / T.V.S. Murthy, Leonardo Brizuela, and Joshua LaBaer -- Cell-free protein synthesis for protein microarrays / Gregory A. Michaud [and others] -- Cell-free protein expression screening and protein immobilization using protein microarrays / Matthew A. Coleman [and others] -- Cell-free protein expression labeling with fluorophores / Jerzy Olejnik -- Cell-free synthesis of defined protein conjugates by site-directed cotranslational labeling / Michael Gerrits [and others] -- C-terminal labeling of proteins using fluorescently conjugated puromycin derivatives / Ichiro Tabuchi -- Translation engineering and synthetic biology / David A. Roth, Liza S.Z. Larsen and G. Wesley Hatfield -- Accelerated protein evolution using ribosome display / Julie Douthwaite, Lutz Jermutus, Ronald Jackson -- Application of in vitro virus (IVV) technique for high-throughput analysis of protein-protein interactions / Etsuko Miyamoto-Sato and Hiroshi Yanagawa
Summary Following its inception in the 1950s, cell-free protein synthesis made a tremendous impact on the basic life sciences. The use of cell-free systems was key to understanding molecular mechanisms underlying one of the most complicated processes found in nature: protein translation. Since this time, aggressive cutting-edge research and stiff commercial competition have driven the development of a variety of systems with increased productivity, improved protein quality and relatively low production costs. As a result, technology has generated myriad applications that have enabled advances in field
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Proteins -- Synthesis.
Genetic translation.
Genetic regulation.
Cell-Free System -- physiology
Protein Biosynthesis
Gene Expression Regulation
Proteins -- chemical synthesis
SCIENCE -- Life Sciences -- Biochemistry.
Genetic regulation
Genetic translation
Proteins -- Synthesis
Form Electronic book
Author Kudlicki, Wieslaw A
Katzen, Federico
Bennett, Robert P. (Robert Paul)
ISBN 9781435614352
1435614356
9781433713224
1433713225
9781587063510
1587063514