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Title In vitro transcription and translation protocols
Edition 2nd ed. / edited by Guido Grandi
Published Totowa, N.J. : Humana Press, ©2007

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Description 1 online resource (xi, 276 pages) : illustrations
Series Methods in molecular biology, 1064-3745 ; 375
Methods in molecular biology (Clifton, N.J.) ; v. 375
Contents Energy systems for ATP regeneration in cell-free protein synthesis reactions / Kara A. Calhoun and James R. Swartz -- Continuous-exchange protein-synthesizing systems / Vladimir A. Shirokov [and others] -- Cell-free production of integral membrane proteins on a preparative scale / Christian Klammt [and others] -- SIMPLEX : Single-molecule PCR-linked in vitro expression : a novel method for high-throughput construction and screening of protein libraries / Suang Rungpragayphan, Tsuneo Yamane, and Hideo Nakano -- Methods for high-throughput materialization of genetic information based on the wheat germ cell-free expression system / Tatsuya Sawasaki [and others] -- Exogenous protein expression in Xenopus oocytes : basic procedures / Elena Bossi, Maria Serena Fabbrini, and Aldo Ceriotti -- In vitro translation to study HIV protease activity / Zene Matsuda [and others]
The protein truncation test in mutation detection and molecular diagnosis / Oliver Hauss and Oliver Müller -- Creation of novel enantioselective lipases by SIMPLEX / Yuichi Koga, Tsuneo Yamane, and Hideo Nakano -- In vitro transcription and translation coupled with two-dimensional electrophoresis for bacterial proteome analysis / Nathalie Norais [and others] -- In vitro screen of bioinformatically selected Bacillus anthracis vaccine candidates by coupled transcription, translation, and immunoprecipitation analysis / Orit Gat, Haim Grosfeld, and Avigdor Shafferman -- Functional expression of Type 1 rat GABA transporter in microinjected Xenopus laevis oocytes / Stefano Giovannardi [and others] -- Production of protein for nuclear magnetic resonance study using the wheat germ cell-free system / Toshiyuki Kohno and Yaeta Endo
Summary A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration, the efficient in vitro production of integral membrane proteins, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. In Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the in vitro transcription and translation to identify proteins in 2D-maps
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Molecular genetics -- Laboratory manuals
Genetic transcription -- Laboratory manuals
Genetic translation -- Laboratory manuals
Genetics -- Technique.
Genetic transcription.
Genetic Techniques
Molecular Biology
Protein Biosynthesis
Transcription, Genetic
SCIENCE -- Life Sciences -- Molecular Biology.
Genetic translation.
Molecular biology.
Protein biosynthesis.
Transcription, Genetic.
Genetic Techniques.
Molecular genetics.
Genetic transcription.
Genetic transcription
Genetic translation
Molecular genetics
Genre/Form Handbooks and manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Grandi, Guido.
ISBN 1588295583
9781588295583
9781597453882
1597453889
128097186X
9781280971860