Title Antimicrobial peptides : methods and protocols / edited by Paul R. Hansen

Published New York : Humana Press : Springer, ©2017

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Description 1 online resource (xiv, 439 pages) : illustrations (some color) Series Methods in molecular biology ; volume no. 1548 Methods in molecular biology (Clifton, N.J.) ; v. 1548. 1064-3745 Contents 880-01 Antimicrobial Peptides: An Introduction -- Tools for designing amphipathic helical antimicrobial peptides -- Chemical Synthesis of Antimicrobial Peptides -- Microwave-Assisted Synthesis of Antimicrobial Peptides -- High Performance Liquid Chromatography and Mass Spectrometry-Based Design of Protolytically Stable Antimicrobial Peptides -- Determination of Structure and Micellar Interactions of Small Antimicrobial Peptides by Solution-State NMR -- Mass spectrometry approaches for determining the structure of antimicrobial peptides -- Molecular Dynamics Simulation and Analysis of the Antimicrobial Peptides-Lipid Bilayers Interactions -- Calorimetry methods to study membrane interactions and perturbations induced by antimicrobial host defense peptides -- Fluorescence and absorbance spectroscopy methods to study membrane perturbations by antimicrobial host defense peptides -- Applying Fluorescence Correlation Spectroscopy to Investigate Peptide-Induced Membrane Disruption -- Macromolecule Biosynthesis Assay and Fluorescence Spectroscopy Methods to Explore Antimicrobial Peptide Mode(s) of Action -- Using Confocal Microscopy and Computational Modeling to Investigate the Cell Penetrating Properties of Antimicrobial Peptides -- Atomic Force Microscopy Study of the Interactions of Indolicidin with Model Membranes and DNA -- Protocols for studying the interaction of MSI-78 with the Membranes of Whole Gram-positive and Gram-negative Bacteria by NMR -- Preparation of membrane models of Gram-negative bacteria and their interaction with Antimicrobial Peptides studied by CD and NMR -- Studying the Interaction of Magainin 2 and Cecropin A with E. coli Bacterial Cells using Circular Dichroism -- Studying the Mechanism of Membrane Permeabilization induced by Antimicrobial Peptides using Patch-clamp Techniques -- Assays for Identifying Inducers of the Antimicrobial Peptide LL-37 -- Methods for Elucidating the Mechanism of Action of Proline-rich and other Non-lytic Antimicrobial Peptides -- The Interaction of Antimicrobial Peptides with the Membrane and Intracellular Targets of Staphylococcus Aureus Investigated by ATP-leakage, DNA-binding Analysis and the Expression of a LexA Controlled Gene, recA -- Methods for Investigating Biofilm Inhibition and Degradation by Antimicrobial Peptides -- Protocols for Studying Inhibition and Eradication of Bacterial Biofilms by Antimicrobial Peptides -- Protocols for Studying Antimicrobial Peptides (AMPs) as Anti-cancer Agents -- Using Oral and Colon Cancer Cells for studying the Anti-cancer properties of Antimicrobial peptides -- Using Disease-Associated Enzymes to Activate Antimicrobial Peptide Prodrugs -- Anti-inflammatory Properties of Antimicrobial Peptides and Peptidomimetics: LPS and LTA Neutralization -- Protocols for Screening Antimicrobial Peptides That Influence Virulence Gene Expression in Staphylococcus aureus -- Methods for in vitro Analysis of Antimicrobial Activity and Toxicity of Anti-keratitis Peptides: Bacterial Viability in tears, MTT and TNF-a release assays -- Methods for In Vivo/ex-vivo Analysis of Antimicrobial Peptides in Bacterial Keratitis: siRNA Knockdown, Colony Counts, Myeloperoxidase, Immunostaining, and RT-PCR Assays -- Haemolytic Activity of Antimicrobial Peptides 880-01/(S Antimicrobial Peptides : an introduction / Evan F. Haney, Sarah C. Mansour, and Robert E.W. Hancock -- Tools for designing amphipathic helical antimicrobial peptides / Davor Juretić, Damir Vukičević, and Alessandro Tossi -- Chemical synthesis of antimicrobial peptides / Lena Münzker, Alberto Oddo, and Paul R. Hansen -- Microwave-assisted synthesis of antimicrobial peptides / Suhas Ramesh, Beatriz G. de la Torre, Fernando Albericio, Hendrik G. Kruger, and Thavendran Govender -- High-performance liquid chromatography and mass spectrometry-based design of proteolytically stable antimicrobial peptides / Mojtaba Bagheri and Robert E.W. Hancock -- Determination of structure and micellar interactions of small antimicrobial peptides by solution-State NMR / Reinhard Wimmer and Lars Erik Uggerhøj -- Mass spectrometry approaches for determining the structure of antimicrobial peptides / Jiongyu Liu and Jianping Jiang -- Molecular dynamics simulation and analysis of the antimicrobial peptide-lipid bilayer interactions / Shima Arasteh and Mojtaba Bagheri -- Calorimetry methods to study membrane interactions and perturbations induced by antimicrobial host defense peptides / Mauricio Arias, Elmar J. Prenner, and Hans J. Vogel -- Fluorescence and absorbance spectroscopy methods to study membrane perturbations by antimicrobial host defense peptides / Mauricio Arias and Hans J. Vogel -- Applying fluorescence correlation spectroscopy to investigate peptide-induced membrane disruption / Kasper Kristensen, Jonas R. Henriksen, and Thomas L. Andresen -- Macromolecule biosynthesis assay and fluorescence spectroscopy methods to explore antimicrobial peptide mode(s) of action / Bimal Jana, Kristin Renee Baker, and Luca Guardabassi -- Using confocal microscopy and computational modeling to investigate the cell-penetrating properties of antimicrobial peptides / Gabriel Del Rio, Edda Klipp, and Andreas Herrmann -- Atomic force microscopy study of the interactions of indolicidin with model membranes and DNA / Peter Fojan and Leonid Gurevich -- Protocols for studying the interaction of MSI-78 with the membranes of whole gram-positive and gram-negative bacteria by NMR / Nury P. Santisteban, Michael R. Morrow, and Valerie Booth -- Preparation of membrane models of gram-negative bacteria and their interaction with antimicrobial peptides studied by CD and NMR / Rickey P. Hicks -- Studying the interaction of magainin 2 and cecropin A with E. coli bacterial cells using circular dichroism / Concetta Avitabile, Luca Domenico D'Andrea, and Alessandra Romanelli -- Studying the mechanism of membrane permeabilization induced by antimicrobial peptides using patch-clamp techniques / Giorgio Rispoli -- Assays for identifying inducers of the antimicrobial peptide LL-37 / Frank Nylén, Peter Bergman, Gudmundur H. Gudmundsson, and Birgitta Agerberth -- Methods for elucidating the mechanism of action of proline-rich and other non-lytic antimicrobial peptides / Monica Benincasa, Giulia Runti, Mario Mardirossian, Renato Gennaro, and Marco Scocchi -- Interaction of antimicrobial peptides with the membrane and intracellular targets of Staphylococcus aureus investigated by ATP leakage, DNA-binding analysis, and the expression of a LexA-controlled gene, recA / Sanne Gottschalk and Line Elnif Thomsen -- Methods for investigating biofilm inhibition and degradation by antimicrobial peptides / Li-av Segev-Zarko and Yechiel Shai -- Protocols for studying inhibition and eradication of bacterial biofilms by antimicrobial peptides / Vijayalekshmi Sarojini -- Protocols for studying antimicrobial peptides (AMPs) as anticancer agents / Laurence Madera and David W. Hoskin -- Using oral and colon cancer cells for studying the anticancer properties of antimicrobial peptides / Teerakul Arpornsuwan, Wimolpak Sriwai, Hathaitip Sritanaudomchai, and Sittiruk Roytrakul -- Using disease-associated enzymes to activate antimicrobial peptide prodrugs / Éanna B. Forde, Graeme Kelly, Hisham Makki, Zahraa Al-Sharshahi, Deirdre Fitzgerald-Hughes, and Marc Devocelle -- Anti-inflammatory properties of antimicrobial peptides and peptidomimetics : LPS and LTA neutralization / Sarah Line Skovbakke and Henrik Franzyk -- Protocols for screening antimicrobial peptides that influence virulence gene expression in Staphylococcus aureus / Martin Saxtorph Bojer, Mara Baldry, and Hanne Ingmer -- Methods for in vitro analysis of antimicrobial activity and toxicity of anti-keratitis peptides : bacterial viability in tears, MTT, and TNF-α release assays / Floriana Cappiello, Bruno Casciaro, Satya Sree Kolar, Hasna Baidouri, Alison M. McDermott, and Maria Luisa Mangoni -- Methods for in vivo/ex vivo analysis of antimicrobial peptides in bacterial keratitis : siRNA knockdown, colony counts, myeloperoxidase, immunostaining, and RT-PCR assays / Satya Sree Kolar, Hasna Baidouri, Maria Luisa Mangoni, and Alison M. McDermott -- Hemolytic activity of antimicrobial peptides / Alberto Oddo and Paul R. Hansen Summary This volume provides basic and advanced laboratory protocols for the non-specialist and the experienced researcher. Chapters are divided into three parts covering synthesis and characterization of AMPs, studying the interaction of AMPs with model systems or bacteria, and assaying selected biological activities of AMPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Antimicrobial Peptides: Methods and Protocols aims to ensure successful results in the further study of this vital field Bibliography Includes bibliographical references and index Notes Online resource; title from PDF title page (SpringerProtocols, viewed December 27, 2016) Subject Peptide antibiotics -- Laboratory manuals Anti-infective agents. Anti-Infective Agents Peptides Anti-infective agents Peptide antibiotics Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Hansen, Paul R., editor LC no. 2016955825 ISBN 9781493967377 1493967371 1493967355 9781493967353

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