| Description |
1 online resource (364 p.) |
| Contents |
Intro -- Foreword -- Preface -- Acknowledgement -- Contents -- Editors and Contributors -- 1: Revolutionizing Genomics: Exploring the Potential of Next-Generation Sequencing -- 1.1 Introduction -- 1.1.1 The Evolution of DNA Sequencing -- 1.2 Next-Generation Sequencing Platforms -- 1.3 Library Preparation and Sequencing Workflow -- 1.3.1 Sample Collection and DNA Extraction -- 1.3.2 Library Preparation Methods -- 1.3.3 Quality Control and Library Quantification -- 1.3.3.1 qPCR -- 1.3.3.2 Fluorometric Quantification -- 1.3.3.3 Bioanalyzer/Fragment Analyzer -- 1.3.4 Sequencing Workflow Overview |
|
1.3.5 Challenges and Best Practices in Library Preparation -- 1.4 Applications of Next-Generation Sequencing -- 1.4.1 Genomic Sequencing -- 1.4.2 Transcriptome Analysis -- 1.4.3 Epigenetic Profiling -- 1.4.4 Metagenomics -- 1.4.5 Single-Cell Sequencing -- 1.4.6 Clinical Applications -- 1.4.7 Forensic Genetics -- 1.5 Data Analysis and Bioinformatics -- 1.5.1 Data Processing and Quality Control -- 1.5.2 Read Mapping and Alignment -- 1.5.3 Variant Calling and Structural Variation Analysis -- 1.5.4 Transcriptome Analysis Tools -- 1.5.5 Epigenetic Data Analysis -- 1.5.6 Metagenomics Data Analysis |
|
1.5.7 Integrative Analysis and Interpretation -- 1.6 Challenges and Opportunity in Future of NGS -- 1.6.1 Metagenomics -- 1.6.2 Shotgun Sequencing of Metagenomes -- 1.6.3 Future Prospect of Metagenomics -- 1.7 Conclusion -- References -- 2: Advances in Structural Bioinformatics -- 2.1 Introduction -- 2.2 Protein Structure -- 2.3 Structure Visualization -- 2.4 DNA Structure Background -- 2.5 Interactions -- 2.6 Calculating Contacts -- 2.7 Protein Data Bank (PDB) -- 2.8 Data Format -- 2.9 Other Structural Databases -- 2.10 Structure Comparison -- 2.10.1 Structural Alignment |
|
2.10.2 Graph-Based Structural Signatures -- 2.10.3 Structure Prediction -- 2.10.4 Comparative Modeling -- 2.10.5 De Novo Modeling -- 2.10.6 Structure Validation -- 2.10.7 Prediction Tools -- 2.10.8 Molecular Docking -- 2.10.9 Virtual Screening -- 2.10.10 Molecular Dynamics -- 2.11 Applications -- 2.12 Tools -- 2.12.1 List of Structural Bioinformatics Tools -- 2.12.1.1 I-TASSER (https://zhanggroup.org/I-TASSER) -- 2.12.1.2 Molecular Operating Environment (https://www.chemcomp.com/Products.htm) -- 2.12.1.3 Structural Bioinformatics Library (https://sbl.inria.fr/) |
|
2.12.1.4 BALLView (https://ball-project.org/ballview/) -- 2.12.1.5 PyMoL (https://pymol.org/2/) -- 2.12.1.6 Visual Molecular Dynamics (https://www.ks.uiuc.edu/Research/vmd/) -- 2.12.1.7 KiNG (http://kinemage.biochem.duke.edu/software/king/) -- 2.12.1.8 STRIDE (Algorithm) (https://webclu.bio.wzw.tum.de/cgi-bin/stride/stridecgi.py) -- 2.12.1.9 DSSP (Algorithm) (https://www.blopig.com/blog/2014/08/dssp/) -- 2.12.1.10 MolProbity (http://molprobity.manchester.ac.uk/) -- 2.12.1.11 PROCHECK (https://web.archive.org/web/20080801065546/http://www.biochem.ucl.ac.uk/̃roman/procheck/procheck.html) |
| Summary |
The second edition of Advances in Bioinformatics presents the latest developments in bioinformatics in gene discovery, genome analysis, genomics, transcriptomics, proteomics, metabolomics, metabolic flux analysis, drug discovery, and drug repurposing. It includes advancements in the applications of bioinformatics in the analysis of non-coding RNA, next-generation sequencing, gene synthesis, genome-scale modeling, high throughput drug screening, precision medicine, automation, artificial intelligence, and machine learning. Additionally, the book highlights some of the areas in which bioinformatics resources and methods are being developed to support the drug discovery pipeline. The chapter also discusses the role of bioinformatics in modeling and simulations of molecular biology systems in pathways identification and design. It is a valuable source of information for beginners in bioinformatics and students, researchers, scientists, clinicians, practitioners, policymakers, and stakeholders who are interested in harnessing the potential of bioinformatics in biomedical and allied sciences |
| Notes |
2.12.1.12 CheShift (http://www.cheshift.com/) |
|
Online resource; title from PDF title page (SpringerLink, viewed February 26, 2024) |
| Subject |
Bioinformatics.
|
| Genre/Form |
Electronic books
|
| Form |
Electronic book
|
| Author |
Singh, Vijai
|
|
Kumar, Ajay
|
| ISBN |
9789819984015 |
|
9819984017 |
|
