| Description |
1 online resource |
| Series |
Woodhead publishing series in biomedicine ; numbers 83 |
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Woodhead Publishing series in biomedicine ; no. 83.
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| Contents |
PrefaceChapter 1: Mechanism, current challenges and new approaches for non viral gene delivery; 1.1 Introduction; 1.2 Current non-viral gene delivery vectors; 1.2.1 Naked DNA; 1.2.2 Cationic lipids; 1.2.2.1 Liposomes; 1.2.2.2 Lipid-coated DNA complexes; 1.2.2.3 Niosomes; 1.2.3 Cationic polymers; 1.2.3.1 Polyethylenimine (PEI); 1.2.3.2 Poly-L-lysine (PLL); 1.2.3.3 Polymethacrylate; 1.2.3.4 Chitosan; 1.2.3.5 Dendrimers; 1.2.3.5.1 Polyamidoamine dendrimers (PAMAM); 1.2.3.5.2 Poly(propylenimine) dendrimers (PPI); 1.2.3.5.3 Poly(L-lysine) dendrimers |
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1.3 Trajectory and mechanism of non-viral vectors1.3.1 Basics of non-viral gene delivery system formulation; 1.3.1.1 Complexes between cationic lipid and DNA (lipoplexes); 1.3.1.2 Complexes between cationic polymer and DNA (polyplexes); 1.3.2 Binding and uptake; 1.3.2.1 Phagocytosis; 1.3.2.2 Clathrin-mediated endocytosis; 1.3.2.3 Caveolae-mediated endocytosis; 1.3.2.4 Macropinocytosis; 1.3.2.5 Non-endocytic pathways; 1.3.3 Characteristics of non-viral gene delivery influencing uptake pathways; 1.3.3.1 Size; 1.3.3.2 Surface charge; 1.3.3.3 Shape; 1.3.3.4 Cell type |
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1.3.4 Endosomal escape and dissociation of complexes1.3.4.1 Lipoplexes; 1.3.4.2 Polyplexes; 1.3.5 Fate in cytoplasm and nuclear entry; 1.4 Conclusion and future perspective; References; Chapter 2: PEI and its derivatives for gene therapy; 2.1 Introduction; 2.2 Criteria for non-viral vectors and general considerations; 2.3 Rationale for the use of PEI for gene therapy; 2.4 PEI Derivatives for gene delivery; 2.4.1 Grafting targeting ligands on PEI; 2.4.2 Chemical modification to shield cationic charge density; 2.4.3 Hydrophobic modifications; 2.4.4 Stimuli-sensitive construct |
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2.4.4.1 Redox-sensitive construct2.4.5 pH-sensitive PEI constructs; 2.4.6 Temperature-sensitive PEI constructs; 2.5 PEGylation of PEI; 2.6 Polyester conjugation on PEI; 2.7 Chitosan conjugation on PEI; 2.8 PLL conjugation on PEI; 2.9 Metallic nanoparticles with PEIs; 2.10 Pharmacokinetics and bio-distribution of PEI delivery vectors; 2.11 Concluding remarks; Acknowledgements; References; Chapter 3: Natural polymers in nucleic acid delivery; 3.1 Introducing cells to exogenous nucleic acids; 3.1.1 Nucleic acids as therapeutics; 3.1.2 Applications in cancer |
| Summary |
Polymers and Nanomaterials for Gene Therapy provides the latest information on gene therapy, a topic that has attracted significant attention over the past two decades for the treatment of inherited and acquired genetic diseases. Major research efforts are currently focused on designing suitable carrier vectors that compact and protect oligonucleotides for gene therapy. The book explores the most recent developments in the field of polymer science and nanotechnology, and how these advancements have helped in the design of advanced materials. Non-viral vector systems, including cationic lipids, polymers, dendrimers, peptides and nanoparticles, are potential routes for compacting DNA for systemic delivery. However, unlike viral analogues that have no difficulty in overcoming cellular barriers and immune defense mechanisms, non-viral gene carriers consistently exhibit significant reduced transfection efficiency due to numerous extra- and intracellular obstacles. Therefore, biocompatibility and potential for large-scale production make these compounds increasingly attractive for gene therapy. This book contains chapters on the engineering of polymers and nanomaterials for gene therapy, and how they can form complexes with DNA and avoid both in vitro and in vivo barriers. Other chapters describe in vitro, ex vivo, in vivo gene therapy studies, and the current issues affecting non-viral gene therapy |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (EBSCO, viewed January 14, 2016) |
| Subject |
Polymers in medicine.
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Nanomedicine.
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Gene therapy.
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Genetic Therapy
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Nanomedicine
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HEALTH & FITNESS -- Holism.
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HEALTH & FITNESS -- Reference.
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MEDICAL -- Alternative Medicine.
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MEDICAL -- Atlases.
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MEDICAL -- Essays.
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MEDICAL -- Family & General Practice.
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MEDICAL -- Holistic Medicine.
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MEDICAL -- Osteopathy.
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Gene therapy
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Nanomedicine
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Polymers in medicine
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| Form |
Electronic book
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| Author |
Narain, Ravin, editor.
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| ISBN |
9780081005217 |
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0081005210 |
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