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Title Xenopus : methods and protocols / edited by Kris Vleminckx
Published New York, NY : Humana Press, 2018

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Description 1 online resource (xi, 302 pages) : illustrations (some color)
Series Methods in molecular biology, 1930-6029 ; volume 1865
Methods in molecular biology (Clifton, N.J.) ; v. 1865. 1064-3745
Contents Husbandry, general care, and transportation of Xenopus laevis and Xenopus tropicalis / Sean McNamara, Marcin Wlizla, and Marko E. Horb -- Generation and care of Xenopus laevis and Xenopus tropicalis embryos / Marcin Wlizla, Sean McNamara, and Marko E. Horb -- Methods for CRISPR/Cas9 Xenopus tropicalis tissue-specific multiplex genome engineering / Thomas Naert and Kris Vleminckx -- Targeted genome engineering in xenopus using the transcription activator-like effector nuclease (TALEN) technology / Tom Van Nieuwenhuysen and Kris Vleminckx -- Genotyping of CRISPR/Cas9 genome edited Xenopus tropicalis / Thomas Naert and Kris Vleminckx -- BATCH-GE : analysis of NGS data for genome editing assessment / Wouter Steyaert, Annekatrien Boel, Paul Coucke, and Andy Willaert -- Simple knock-in system for xenopus via microhomology mediated end joining repair / Ken-ich T. Suzuki, Yuto Sakane, Miyuki Suzuki, and Takashi Yamamoto -- How to generate non-mosaic CRISPR/Cas9 mediated knock-in and mutations in F0 Xenopus through the host-transfer technique / Emmanuel Tadjuidje and Sang-Wook Cha -- Targeted electroporation in the CNS in Xenopus embryos / Hovy Ho-Wai Wong and Christine E. Holt -- Conditional chemogenetic ablation of photoreceptor cells in Xenopus retina / Albert Chesneau, Odile Bronchain, and Muriel Perron -- Cancer models in Xenopus tropicalis by CRISPR/Cas9 mediated knockout of tumor suppressors / Thomas Naert and Kris Vleminckx -- CRISPR/Cas9 F0 screening of congenital heart disease genes in Xenopus tropicalis / Engin Deniz, Emily K. Mis, Maura Lane, and Mustafa K. Khokha -- Quantitative proteomics of Xenopus embryos I, sample preparation / Meera Gupta, Matthew Sonnett, Lillia Ryazanova, Marc Presler, and Martin Wühr -- Quantitative proteomics for Xenopus embryos II, data analysis / Matthew Sonnett, Meera Gupta, Thao Nguyen, and Martin Wühr -- Dye electroporation and imaging of calcium signaling in Xenopus nervous system / Lukas Weiss, Thomas Offner, Thomas Hassenklöver, and Ivan Manzini -- X-FaCT : xenopus-fast clearing technique / Pierre Affaticati, Sébastien Le Mével, Arnim Jenett, Laurie Rivière, Elodie Machado, Bilal B. Mughal, and Jean-Baptiste Fini -- Cell cycle analysis of the embryonic brain of fluorescent reporter Xenopus tropicalis by flow cytometry / Rivka Noelanders and Kris Vleminckx -- Manipulating and analyzing cell type composition of the Xenopus mucociliary epidermis / Peter Walentek -- Evaluating blood cell populations in Xenopus using flow cytometry and differential counts by cytospin / Jacques Robert, Eva-Stina Edholm, and Francisco De Jesus Andino -- Isolation and culture of amphibian (Xenopus laevis) sub-capsular liver and bone marrow cells / Amulya Yaparla and Leon Grayfer -- Isolation and primary culture methods of adult and larval myogenic cells from Xenopus laevis / Kazi Taheruzzaman and Akio Nishikawa
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed September 5, 2018)
Subject Xenopus -- Laboratory manuals
Xenopus -- Genetics -- Laboratory manuals
Medical genetics.
Medical -- Genetics.
Xenopus
Xenopus -- Genetics
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Vleminckx, Kris, editor
ISBN 9781493987849
1493987844