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E-book

Title Plant nitric oxide : methods and protocols / edited by Kapuganti Jagadis Gupta
Published New York : Humana Press : Springer, ©2016

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Description 1 online resource (xi, 203 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; volume no. 1424
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 1424. 1064-3745
Springer protocols (Series)
Contents Overview of methods in plant nitric oxide (NO) research : why do we always need to use multiple methods? / Hideo Yamasaki [and others] -- Chemiluminescence detection of nitric oxide from roots, leaves, and root mitochondria / Aakanksha Wany [and others] -- Nitric oxide measurement from purified enzymes and estimation of scavenging activity by gas Phase chemiluminescence method / Aprajita Kumari [and others] -- Localization of nitric oxide in wheat roots by DAF fluorescence / Aakanksha Wany and Kapuganti Jagadis Gupta -- Nitric oxide (NO) measurements in stomatal guard cells / Srinivas Agurla, Gunja Gayatri, and Agepati S. Raghavendra -- Methods to detect nitric oxide in plants : are DAFs really measuring NO? / Stefan Ruemer [and others] -- Novel protocol for detection of nitric oxide in plants / Prachi Jain, Anisha David, and Satish C. Bhatla -- Detection of nitric oxide by electron paramagnetic resonance spectroscopy : spin-trapping with iron-dithiocarbamates / Luisa B. Maia and José J.G. Moura -- Measurement of nitric oxide (NO) generation rate by chloroplasts employing electron spin resonance (ESR) / Andrea Galatro and Susana Puntarulo -- Laser-based methods for detection of nitric oxide in plants / Julien Mandon [and others] -- Electrochemical detection of nitric oxide in plant cell suspensions / Sophie Griveau [and others] -- Quantification and localization of S-nitrosothiols (SNOs) in higher plants / Juan B. Barroso [and others] -- Analysis of the expression and Activity of nitric oxide synthase from marine photosynthetic microorganisms / Noelia Foresi [and others] -- Identification of S-nitrosothiols by the sequential cysteine blocking technique / Rafael A. Homem [and others] -- Detection of S-nitrosoglutathione reductase activity in plants / Lucie Kubienová [and others] -- Detection of peroxynitrite in plants exposed to bacterial infection / Diana Bellin, Massimo Delledonne, and Elodie Vandelle
Summary With numerous methods available for the quantification of nitric oxide (NO), this detailed book explores their advantages and disadvantages in order to provide a foundation for further research in plant nitric oxide. After providing a useful practical guide to choosing a technique for measuring NO from plant materials, the book continues with chapters on chemiluminescence, diaminofluorescence (DAF), EPR spectroscopy, a laser-based method, as well as many other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory methods, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Nitric Oxide: Methods and Protocols serves as a valuable guide to all researchers working and intending to work in the field of plant nitric oxide research
Bibliography Includes bibliographical references and index
Subject Plants.
Nitric oxide.
Nitric-oxide synthase.
Plants -- enzymology
Nitric Oxide -- biosynthesis
Nitric Oxide Synthase
Botany & plant sciences.
Science -- Life Sciences -- Botany.
Nitric-oxide synthase
Nitric oxide
Plants
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Kapuganti, Jagadis G., editor.
ISBN 9781493936007
149393600X
1493935984
9781493935987