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Book Cover

Title Epstein-Barr virus protocols / edited by Joanna B. Wilson and Gerhard H.W. May
Published Totowa, N.J. : Humana Press, ©2001


Description 1 online resource (xiv, 438 pages) : illustrations
Series Methods in molecular biology ; 174
Methods in molecular biology (Clifton, N.J.) ; v. 174
Contents Genome and transcript analyses -- Viral detection -- Culture methods -- Immune assays -- Viral protein detection -- Protein-protein, protein-DNA and protein-RNA interactions -- Protein activity assays -- Tissue and in vivo protocols
Epstein-Barr virus: the B95-8 strain map -- Analysis of replication of oriP-based plasmids by quantitative, competitive PCR -- Granulocyte and granulocyte macrophage colony-stimulating factors as therapy in human and veterinary medicine -- Analysis of the expression and function of the EBV-encoded small RNAs, the EBERs, in heterologous cells -- Prevention of hepatocellular carcinoma in chronic viral hepatitis patients with cirrhosis by carotenoid mixture -- In situ detection of Epstein-Barr virus DNA and viral gene products -- Detection of EBV infection at the single-cell level. Precise quantitation of virus-infected cells in vivo -- The effects of pH on DNA methylation state: In vitro and post-mortem brain studies -- Autolink: Automated sequential resonance assignment of biopolymers from NMR data by relative-hypothesis-prioritization-based simulated logic -- Introduction of plasmid vectors into cells via electroporation -- The maize AB10 meiotic drive system maps to supernumerary sequences in a large complex haplotype -- Hemozoin-free plasmodium falciparum mitochondria for physiological and drug susceptibility studies -- Seasonal and spatial community dynamics in the meromictic Lake Cadagno -- Use of fluorescent dyes as molecular probes for the study of multidrug resistance -- Qualitative detection of apoptotic cells assessed by DNA fragmentation -- A novel method of labeling and characterizing migrating neurons in the developing central nervous system -- Effect of chronic ethanol ingestion on [alpha]-mannosidase isoenzymes in rat liver -- Pulmonary vascular endothelial growth factor-C in development and lung injury in preterm infants -- Comparative evaluation of positive tests to Mycobacterium avium subsp. Paratuberculosis in clinically healthy sheep and goats in south-west Greece using molecular techniques, serology, and culture -- Heritable endosymbionts of drosophila -- Genetic mapping in a natural population of collared flycatchers (Ficedula albicollis): Conserved synteny but gene order rearrangements on the avian Z chromosome -- Utilization of polymerase chain reaction and selective media cultivation to identify legionella in Taiwan spring water samples -- The genetic basis of head and neck carcinoma -- Multiple uses of liquepipets in southern, northern, and western blots -- Pharmacogenetics of antipsychotic-induced weight gain -- From imaging to understanding: Frontiers in live cell imaging, Bethesda, MD, April 19-21, 2006 -- Accumulation of recessive lethal mutations in saccharomyces cerevisiae mlh1 mismatch repair mutants is not associated with gross chromosomal rearrangements -- Pkcalpha: A versatile key for decoding the cellular calcium toolkit -- An efficient genetic screen in drosophila to identify nuclear-encoded genes with mitochondrial function -- Pharmacogenetic studies of alcohol self-administration and withdrawal -- Thomas G. Orr memorial lectureship. Colon cancer from etiology to prevention -- Outbreak of trichinellosis associated with eating cougar jerky -- In vivo models of prostate cancer metastasis to bone -- Variable populations within variable populations: Quantifying mitochondrial heteroplasmy in natural populations of the gynodioecious plant silene vulgaris -- Enrichment of fetal trophoblast cells from the maternal peripheral blood followed by detection of fetal deoxyribonucleic acid with a nested X/Y polymerase chain reaction -- Real-time FRET PCR assay for salmonella enterica serotype detection in food -- Large-scale gene expression differences across brain regions and inbred strains correlate with a behavioral phenotype -- Malate dehydrogenase from chlorobium vibrioforme, chlorobium tepidum, and heliobacterium gestii: Purification, characterization, and investigation of dinucleotide binding by dehydrogenases by use of empirical methods of protein sequence analysis -- Long-term persistence of ciprofloxacin-resistant haemophilus influenzae in patients with cystic fibrosis -- Isolation and comparison of the paracrystalline surface layer proteins of freshwater caulobacters -- Purification and partial characterization of transglutaminase from physarum polycephalum -- An operon of bacillus subtilis motility genes transcribed by the sigma D form of RNA polymerase -- Physical mapping of repetitive extragenic palindromic sequences in escherichia coli and phylogenetic distribution among escherichia coli strains and other enteric bacteria -- Antagonism of antiviral and allogeneic activity of a human public CTL clonotype by a single altered peptide ligand: Implications for allograft rejection -- [Gamma] [delta] T cells respond directly to pathogen-associated molecular patterns -- Resolution of chromosomes III and VI of Aspergillus nidulans by pulsed-field gel electrophoresis shows that the penicillin biosynthetic pathway genes PCBAB, PCBC, and pende are clustered on chromosome VI (3.0 megabases) -- In silico identification of supertypes for class II MHCS -- Cloning of a metalloprotease gene involved in the virulence mechanism of vibrio anguillarum -- The lethal phenotype caused by null mutations in the escherichia coli HTRB gene is suppressed by mutations in the ACCBC operon, encoding two subunits of acetyl coenzyme a carboxylase
Summary The application of recombinant DNA technology to the analysis of Epstein-Barr virus (EBV) is rapidly developing sufficient insight into the virus-host interaction, so that its role in disease pathology is now often discernible and can increasingly be interdicted. In Epstein-Barr Virus Protocols, Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus, along with a series of valuable immunology, cell biology, and transgenic mouse protocols. Described in step-by-step detail by experts who use them regularly, these readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, the authors provide information on EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels. Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV-associated diseases, including several forms of human cancer
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Epstein-Barr virus -- Laboratory manuals
Human beings.
Herpesvirus 4, Human -- physiology
Herpesvirus 4, Human -- isolation & purification
Immunoassay -- methods
Viral Proteins -- chemistry
Viral Proteins -- genetics
Virus Cultivation -- methods
Homo sapiens (species)
MEDICAL -- Microbiology.
Epstein-Barr virus
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Wilson, Joanna B
May, Gerhard H. W
ISBN 9781592592272