| Description |
1 online resource (1 PDF file (xi, 233 pages) : illustrations |
| Series |
Methods in molecular biology, 1940-6029 ;; volume 2170 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ;; v. 2170. 1064-3745
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Springer protocols (Series) 1949-2448
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| Summary |
This updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms. RNA editing and the computational data processing for genome-wide datasets is also discussed. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls |
| Notes |
1. CRISPR-Cas RNA Targeting Using Transient Cas13a Expression in Nicotiana benthamiana Veerendra Sharma, Wenguang Zheng, Jun Huang, and David E. Cook 2. Strand-Specific RNA-Seq Applied to Malaria Samples Xueqing Maggie Lu and Karine Le Roch 3. Laser Microdissection of Cells and Isolation of High-Quality RNA after Cryosectioning Marta Barcala, Carmen Fenoll, and Carolina Escobar 4. Detection of RNA in Ribonucleoprotein Complexes by Blue Native Northern Blotting Lena Krüßel, Steffen Ostendorp, Anna Ostendorp, and Julia Kehr 5. Quantitative Analysis of Plant miRNA Primary Transcripts Jakub Dolata, Andrzej Zielezinski, Agata Stepien, Katarzyna Kruszka, Dawid Bielewicz, Andrzej Pacak, Artur Jarmolowski, Wojciech Karlowski, and Zofia Szweykowska-Kulinska 6. A Revised Adaptation of the Smart-Seq2 Protocol for Single Nematode RNA-Seq Dennis Chang, Lorrayne Serra, Dihong Lu, Ali Mortazavi, and Adler Dillman 7 |
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Analysis of RBP Regulation and Co-Regulation of mRNA 3' UTR Regions in a Luciferase Reporter System Erin L. Sternburg and Fedor V. Karginov 8. Extraction of Small RNAs by Titanium Dioxide Nanofibers Luis A. Jimenez and Wenwan Zhong 9. Identification of MicroRNAs and Natural Antisense Transcript-Originated Endogenous siRNAs from Small-RNA Deep Sequencing Data Weixiong Zhang, Xuefeng Zhou, Xiang Zhou, and Jing Xia 10. Purification and Analysis of Chloroplast RNAs in Arabidopsis Huan Wang and Hailing Jin 11. In Situ Detection of Mature miRNAs in Plants Using LNA-Modified DNA Probes Xiaozhen Yao, Hai Huang, and Lin Xu 12. Northern Blotting Technique for Detection and Expression Analysis of mRNAs and Small RNAs Ankur R. Bhardwaj, Ritu Pandey, Manu Agarwal, and Surekha Katiyar-Agarwal 13 |
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Isolation of Insect Bacteriocytes as a Platform for Transcriptomic Analyses Mélanie Ribeiro Lopes, Pierre Simonet, Gabrielle Duport, Karen Gaget, Séverine Balmand, Akiko Sugio, Jean-Christophe Simon, Nicolas Parisot, and Federica Calevro 14. Small RNA Isolation and Library Construction for Expression Profiling of Small RNAs from Neurospora crassa and Fusarium oxysporum and Analysis of Small RNAs in Fusarium oxysporum-Infected Plant Root Tissue Shou-Qiang Ouyang, Gyungsoon Park, Hui-Min Ji, and Katherine A. Borkovich 15. Studying RNA-Protein Interaction Using Riboproteomics Sonali Chaturvedi and A.L.N. Rao 16. Small RNA Extraction and Quantification of Isolated Fungal Cells from Plant Tissue by the Sequential Protoplastation Qiang Cai and Hailing Jin |
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Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force. WlAbNL |
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Online resource;; title from PDF title page (SpringerLink, viewed November 2, 2021) |
| Subject |
Life sciences.
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Biochemistry.
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RNA -- analysis
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RNA -- genetics
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Gene Expression Profiling
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Biological Science Disciplines
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Biochemistry
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biological sciences.
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biochemistry.
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Biology, life sciences.
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Biochemistry.
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Science -- Life Sciences -- Biology -- General.
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Science -- Life Sciences -- Biochemistry.
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Biochemistry.
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Life sciences.
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Jin, Hailing, editor
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Kaloshian, Isgouhi, editor
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| ISBN |
9781071607435 |
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107160743X |
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