Title Single cell metabolism : methods and protocols / edited by Bindesh Shrestha

Published New York, NY : Humana Press, 2020

Click on the following:
Springer eBooks

Copies

Description 1 online resource (xi, 226 pages) : illustrations (some color) Series Methods in molecular biology, 1940-6029 ;; volume 2064 Methods in molecular biology (Clifton, N.J.) ;; v. 2064. 1064-3745 Contents Preface...Table of Contents...Contributing Authors... 1. Single Cell Metabolomics by Mass SpectrometryBindesh Shrestha 2. Identification of Metabolites in Single Cells by Ion Mobility Separation and Mass SpectrometryLinwen Zhang, Linda L. Allworth, and Akos Vertes 3. Analysis of Lipids in Single Cells and Organelles using Nanomanipulation-Coupled Mass SpectrometryMandy S. Phelps and Guido F. Verbeck 4. Development of Pico-ESI-MS for Single-Cell Metabolomics AnalysisZhenwei Wei, Xiaochao Zhang, Xingyu Si, Xiaoyun Gong, Sichun Zhang, and Xinrong Zhang 5. Single-Probe Mass Spectrometry Analysis of Metabolites in Single CellsNing Pan, Wei Rao, and Zhibo Yang 6. Applications of MicroArrays for Mass Spectrometry (MAMS) in Single Cell MetabolomicsAlfredo J. Ibáñez and Ales Svatos 7. Laser Capture Microdissection -- Liquid Vortex Capture -- Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae CellsJohn F. Cahill and Vilmos Kertesz 8. Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS ImagingDhaka Ram Bhandari, Giulia Coliva, Maria Fedorova, and Bernhard Spengler 9. A MALDI-MS Methodology for Studying Metabolic Heterogeneity of Single Cells in a PopulationJasmin Krismer, Jens Sobek, Robert F. Steinhoff, Rolf Brönnimann, Martin Pabst, and Renato Zenobi 10. Sample Preparation and Analysis of Single Cells using High Performance MALDI FTICR Mass SpectrometryBo Yang, Tina Tsui, Richard M. Caprioli, and Jeremy L. Norris 11. Toward Single Cell Molecular Imaging by Matrix-Free Nanophotonic Laser Desorption Ionization Mass SpectrometrySylwia A. Stopka and Akos Vertes 12. Compact Quantum Dots for Quantitative CytologyPhuong Le, Shweta Chitoor, Chunlai Tu, Sung Jun Lim, and Andrew M. Smith 13. Ambient Lipidomic Analysis of Single Mammalian Oocytes and Preimplantation Embryos using Desorption Electrospray Ionization (DESI) Mass SpectrometryChristina R. Ferreira, Valentina Pirro, Alan K. Jarmusch, Clint M. Alfaro, and R. Graham Cooks 14. Spatial Mapping of Cellular Metabolites using DESI Ion Mobility Mass SpectrometryAnthony Midey, Hernando Olivos, and Bindesh Shrestha 15. Open-Source Software Tools, Databases, and Resources for Single Cell and Single Cell-Type MetabolomicsBiswapriya B. Misra 16. Ten Major Future Challenges in Single Cell MetabolomicsBindesh Shrestha Summary This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis;; laser capture microdissection;; ambient single cell metabolite profile (DESI and LAESI);; and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful I series format, the chapters consist of introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls Bibliography Includes bibliographical references and index Notes Online resource;; title from PDF title page (SpringerLink, viewed October 1, 2019) Subject Cell metabolism -- Laboratory manuals Single-Cell Analysis Metabolomics -- methods Cell metabolism Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Shrestha, Bindesh, editor ISBN 9781493998319 1493998315 1493998293 9781493998296

  Permalink