| Description |
1 online resource (xiv, 405 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ;; volume 2061 |
|
Methods in molecular biology (Clifton, N.J.) ;; v. 2061. 1064-3745
|
| Contents |
Part I Cytological and Imaging Approaches to Study Arabidopsis Meiosis 1. A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis / Maria Ada Prusicki, Yuki Hamamura, and Arp Schnittger 2. Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes / Santiago Valentín Galvan Gordillo, Rocio Escobar-Guzman, Daniel Rodriguez-Leal, Jean-Philippe Vielle-Calzada, and Arnaud Ronceret 3. How to Perform an Accurate Analysis of Metaphase I Chromosome Configurations in Autopolyploids of Arabidopsis thaliana / Pablo Parra-Nunez, Mónica Pradillo, and Juan Luis Santos 4. Cytological Characterization of Arabidopsis arenosa Polyploids by SIM / Chris Morgan and Eva Wegel 5. Targeted Analysis of Chromatin Events (TACE) / Jason Sims, Changbin Chen, Peter Schlögelhofer, and Marie-Therese Kurzbauer 6. Whole Mount Immuno-FISH on Arabidopsis Meiocytes (WhoMI-FISH) / Jason Sims, Jihed Chouaref, and Peter Schlögelhofer Part II Cytological and Imaging Approaches to Study Meiosis in Other Species 7. Using Genome In Situ Hybridization (GISH) to Distinguish the Constituent Genomes of Brassica nigra and B. rapa in the Hybrid B. juncea / Zeeshan Shamim and Susan J. Armstrong 8. Preparing Maize Synaptonemal Complex Spreads and Sequential Immunofluorescence and Fluorescence In Situ Hybridization / Stephen M. Stack, Lindsay A. Shearer, Leslie D. Lohmiller, and Lorinda K. Anderson 9. Cytological Techniques to Study Cytomixis in Plant Male Meiosis / Sergey R. Mursalimov, Yuriy V. Sidorchuk, and Elena V. Deineko 10. Analysis of Meiosis in Non-Model Tropical Plants: The Case of Carica papaya LinnJosé Mora-Calderón, Kalani Scott-Moraga, and Pablo Bolaños-Villegas 11. Analytical Methodology of Meiosis in Auto- and Allopolyploid PlantsPilar Prieto and Tomás Naranjo 12. Multi-Colored Fluorescent In Situ Hybridization to Assess Pairing Configurations at Metaphase I in Brassica HybridsVirginie Huteau and Olivier Coriton 13. Surface Spreading Technique in Plant Meiocytes for Analysis of Synaptonemal Complex by Electron MicroscopyNieves Cuñado 14. Quantification of Synapsis using Immunolocalization in Embedded Nuclei of LoliumDylan W. Phillips 15. Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High Resolution MicroscopyBenoit Darrier, Mikel Arrieta, Sybille U. Mittmann, Pierre Sourdille, Luke Ramsay, Robbie Waugh, and Isabelle Colas Part III Molecular and Biochemical Approaches to Study Meiosis 16. Chromatin Immunoprecipitation of Meiotically Expressed Proteins from Arabidopsis thaliana FlowersChristophe Lambing, Kyuha Choi, Alexander R. Blackwell, and Ian R. Henderson 17. Isolating Male Meiocytes from Maize and Wheat for '-omics' AnalysesStefanie Dukowic-Schulze, Nelson Garcia, Arun S.K Shunmugam, Sateesh Kagale, and Changbin Chen 18. How to Study the Proteomes and Phosphoproteomes of Anther and PollenChi Zhang, Xiaobing Fen, Menghui Hu, and Zaibao Zhang 19. Rice Female Meiosis: Genome-Wide mRNA, Small RNA and DNA Methylation Analysis during Ovule Development / Helian Liu, Aqin Cao, Liyu Yang, and Jianbo Wang 20. Quantification of Recombination Rate and Segregation Distortion by Genotyping and Sequencing of Single Pollen Nuclei / Steven Dreissig, Jörg Fuchs, Axel Himmelbach, Martin Mascher, and Andreas Houben Part IV Further Approaches to Study Plants Meiosis and Beyond 21. Identifying and Isolating Meiotic Mutants in Polyploid Brassica Crops / Marina Pfalz, Adrián Gonzalo, Nicolas Christophorou, Aurélien Blary, Aurélie Berard, Nadia Bessoltane, Emilie Montes, Lydia Jaffrelo, Charles Poncet, Marie-Christine Le Paslier, Nathalie Nesi, Delphine Charif, and Eric Jenczewski 22. A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene-Silencing (VIGS) / Stuart Desjardins, Kostya Kanyuka, and James D. Higgins 23. Induction and Characterization of Diploid Pollen Grains in Arabidopsis thaliana / Nico De Storme and Danny Geelen 24. Analysis of Pollen Grains by Immunostaining and FISH in Triticeae Species / Dan D. Wu, Alevtina Ruban, Twan Rutten, Yong H. Zhou, and Andreas Houben 25. Analysing Somatic DNA Repair in Arabidopsis Meiotic Mutants / Annika Dorn and Holger Puchta 26. A Modular Tray Growth System for Barley / Mikel Arrieta, Isabelle Colas, Malcolm Macaulay, Robbie Waugh, and Luke Ramsay 27. In planta Delivery of Chemical Compounds into Barley Meiocytes -- EdU as Compound Example / Yun-Jae Ahn, Maria Cuacos, Mohammad A. Ayoub, Judith Kappermann, Andreas Houben, and Stefan Heckmann |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource;; title from PDF title page (SpringerLink, viewed October 8, 2019) |
| Subject |
Plant genetics -- Laboratory manuals
|
|
Meiosis -- Laboratory manuals
|
|
Plant cells and tissues -- Laboratory manuals
|
|
Meiosis
|
|
Plant cells and tissues
|
|
Plant genetics
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Pradillo, Mónica, editor
|
|
Heckmann, Stefan, editor
|
| ISBN |
9781493998180 |
|
1493998188 |
|
