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E-book

Title Design and delivery of SiRNA therapeutics / edited by Henrik J. Ditzel, Martina Tuttolomondo, Sakari Kauppinen
Published New York, NY : Humana Press, [2021]

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Description 1 online resource (xii, 469 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; 2282
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; 2282.
Springer protocols (Series)
Contents RNA Interference: Story and Mechanisms -- Selection of Chemical Modifications in the siRNA Seed Region That Repress Off-target Effect -- Stick-based Methods for Aptamer-mediated siRNA Targeted Delivery -- Evaluation of siRNA Stability and Interaction With Serum Components Using an Agarose Gel-Based Single-Molecule FRET Labeling Method -- Development of siRNA Therapeutics for the Treatment of Liver Diseases -- siRNA Design and GalNAc-Empowered Hepatic Targeted Delivery -- Synthesis of GalNAc-Oligonucleotide Conjugates Using GalNAc Phosphoramidite and Triple-GalNAc CPG Solid Support -- Preparation of Lipid-Conjugated siRNA Oligonucleotides for Enhancing Gene Inhibition in Mammalian Cells -- Engineering of Solid Dosages Forms of siRNA-loaded Lipidoid-Polymer Hybrid Nanoparticles using a quality by Design Approach -- Preparation and Characterization of siRNA-loaded Liposomes -- SiRNA Vehicles for High Endosomal Escapability -- SiRNA Design and Delivery Based on Carbon Nanotubes -- MRI-Visible Nanovehicle for Efficient siRNA Delivery -- Cationic Dendrimers for siRNA Delivery: An Overview of Methods for in Vitro / In Vivo Characterization -- ITC for Characterization of Self-Assembly Process of Cationic Dendrons for siRNA Delivery -- Cationic Dendrimers for siRNA Delivery -- Computational Approaches for Characterization -- Fundamental and Practical Aspects in the Formulation of Colloidal Polyelectrolyte Complexes of Chitosan and siRNA -- Cell-Penetrating Peptides Delivering siRNAs: An Overview -- Non-Covalent Encapsulation of siRNA with Cell-penetrating Peptides -- siRNA Therapeutics for Protein Misfolding Diseases of the Central Nervous System -- siRNA Loaded-exosomes -- Production and Characterization of Anti-CCR5 siRNA-Loaded Polycaprolactone Nanoparticles for Topical Pre-Exposure Prophylaxis -- siRNA Therapeutics in Ocular Diseases -- Delivery of siRNA to the Eye: Protocol for a Feasibility Study to Assess Novel Delivery System for Topical Delivery of siRNA Therapeutics to the Ocular Surface
Summary This volume details protocols on rationale design of therapeutic siRNA molecules and its encapsulation with smart vehicles to overcome the barriers to an effective administration in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Design and Delivery of SiRNA Therapeutics aims to ensure successful results in the further study of this vital field. This volume details protocols on rationale design of therapeutic siRNA molecules and its encapsulation with smart vehicles to overcome the barriers to an effective administration in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Design and Delivery of SiRNA Therapeutics aims to ensure successful results in the further study of this vital field
Notes Includes index
Online resource; title from PDF title page (SpringerLink, viewed May 10, 2021)
Subject Small interfering RNA -- Laboratory manuals
RNA editing -- Laboratory manuals
Drug development -- Laboratory manuals
Drug development
RNA editing
Small interfering RNA
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Ditzel, Henrik J., editor
Tuttolomondo, Martina, editor
Kauppinen, Sakari, editor
ISBN 9781071612989
1071612980