Title Receptor binding techniques / edited by Anthony P. Davenport

Edition 3rd ed Published New York, NY : Humana ; London : Springer [distributor], 2012

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Description 1 online resource (xii, 309 pages) : illustrations Series Methods in molecular biology, 1940-6029 ; v. 897 Methods in molecular biology (Clifton, N.J.) ; volume 897. 1064-3745 Contents 880-01 Receptor databases and computational websites for ligand binding / Brinda K. Rana, Philip E. Bourne, and Paul A. Insel -- How to use the IUPHAR receptor database to navigate pharmacological data / Chidochangu P. Mpamhanga [and others] -- Radioligand binding assays and their analysis / Janet J. Maguire, Rhoda E. Kuc, and Anthony P. Davenport -- Use of scintillation proximity assay to measure radioligand binding to immobilized receptors without separation of bound from free ligand / Jenny Berry, Molly Price-Jones, and Barbara Killian -- Visualization and analysis of vascular receptors using confocal laser scanning microscopy and fluorescent ligands / Craig J. Daly, Ingela Parmryd, and John C. McGrath -- Dissecting the pharmacology of G protein-coupled receptor signaling complexes using bimolecular fluorescence complementation / Laura E. Kilpatrick and Nicholas D. Holliday -- Live cell imaging of G protein-coupled receptors / Anke Teichmann [and others] -- Characterization of G-protein coupled receptor modulators using homogeneous cAMP assays / Daniel L. Bassoni [and others] -- Measurements of [beta]-arrestin recruitment to activated seven transmembrane receptors using enzyme complementation / Daniel L. Bassoni [and others] -- Quantitative phosphor imaging autoradiography of radioligands for positron emission tomography / Peter Johnstrom, Joseph L. Bird, and Anthony P. Davenport -- Dynamic in vivo imaging of receptors in small animals using positron emission tomography / Peter Johnstrom [and others] -- Cellular localization of receptors using antibodies visualized by light and dual labeling confocal microscopy / Anthony P. Davenport and Rhoda E. Kuc -- Detection of mRNA encoding receptors by in situ and northern hybridization / Alessandra P. Princivalle [and others] 880-01/(S Receptor databases and computational websites for ligand binding / Brinda K. Rana, Philip E. Bourne, and Paul A. Insel -- How to use the IUPHAR receptor database to navigate pharmacological data / Chidochangu P. Mpamhanga [and others] -- Radioligand binding assays and their analysis / Janet J. Maguire, Rhoda E. Kuc, and Anthony P. Davenport -- Use of scintillation proximity assay to measure radioligand binding to immobilized receptors without separation of bound from free ligand / Jenny Berry, Molly Price-Jones, and Barbara Killian -- Visualization and analysis of vascular receptors using confocal laser scanning microscopy and fluorescent ligands / Craig J. Daly, Ingela Parmryd, and John C. McGrath -- Dissecting the pharmacology of G protein-coupled receptor signaling complexes using bimolecular fluorescence complementation / Laura E. Kilpatrick and Nicholas D. Holliday -- Live cell imaging of G protein-coupled receptors / Anke Teichmann [and others] -- Characterization of G-protein coupled receptor modulators using homogeneous cAMP assays / Daniel L. Bassoni [and others] -- Measurements of β-arrestin recruitment to activated seven transmembrane receptors using enzyme complementation / Daniel L. Bassoni [and others] -- Quantitative phosphor imaging autoradiography of radioligands for positron emission tomography / Peter Johnstrom, Joseph L. Bird, and Anthony P. Davenport -- Dynamic in vivo imaging of receptors in small animals using positron emission tomography / Peter Johnstrom [and others] -- Cellular localization of receptors using antibodies visualized by light and dual labeling confocal microscopy / Anthony P. Davenport and Rhoda E. Kuc -- Detection of mRNA encoding receptors by in situ and northern hybridization / Alessandra P. Princivalle [and others] Summary A broad definition of a receptor is a specialized protein on or in a cell that recognizes and binds a specific ligand to undergo a conformational change, leading to a physiological response or change in cell function. A ligand can be an endogenous neurotransmitter, hormone, paracrine/autocrine factor, or a synthetic drug that may function as an agonist or antagonist. The third edition of Receptor Binding Techniques expands upon the methods and techniques used for studying receptors in silico, in vitro and in vivo. Comprehensive chapters describe how to use online resources for experimental research such as prediction of receptor-ligand interactions and mine the IUPHAR receptor database. Classical techniques of radioligand binding, quantitative autoradiography and their analyses are complemented by the use of immunocytochemistry for the cellular localization of receptor protein and hybridization to detect receptor mRNA. Protocols using fluorescent labeled ligands are described to visualise receptors in living cells, their interaction with beta-arrestin to measure ligand-induced internalisation and green fluorescent protein to study trafficking. Non-radioactive, chemiluminescent cAMP and arrestin assays facilitate the identification of novel 'biased agonists'. Detailed methods are provided for in vivo imaging of receptors using positron emission tomography (PET). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Receptor Binding Techniques, Third Edition, aids scientists in continuing to study receptor binding Analysis eiwitten proteins moleculaire biologie molecular biology liganden ligands Molecular Biology (General) Moleculaire biologie (algemeen) Notes Previous edition: 2005 Bibliography Includes bibliographical references and index Notes English Print version record Subject Receptor-ligand complexes -- Research -- Laboratory manuals Ligand binding (Biochemistry) -- Laboratory manuals Cell receptors -- Research -- Laboratory manuals Proteins. Metabolism. Cell receptors. Molecular structure. Membrane proteins. Diagnosis. Radioligand assay. Methodology. Binding sites (Biochemistry) Drug receptors. Carrier proteins. Biochemical Phenomena Proteins Metabolism Diagnostic Techniques, Endocrine Diagnostic Techniques, Radioisotope Investigative Techniques Laboratory Techniques and Procedures Receptors, Cell Surface Molecular Structure Membrane Proteins Diagnostic Techniques and Procedures Analytical, Diagnostic and Therapeutic Techniques and Equipment Amino Acids, Peptides, and Proteins Chemical Phenomena Diagnosis Chemicals and Drugs Phenomena and Processes Radioligand Assay Methods Binding Sites Receptors, Drug Receptors, G-Protein-Coupled Carrier Proteins protein. metabolism (biological concept) molecular structure. diagnosis. methodology. Radioligand assay Proteins Molecular structure Methodology Metabolism Membrane proteins Drug receptors Diagnosis Cell receptors Carrier proteins Binding sites (Biochemistry) Cell receptors -- Research Ligand binding (Biochemistry) Genre/Form Laboratory manuals Form Electronic book Author Davenport, Anthony P. ISBN 9781617799099 1617799092

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