Title The complement system : methods and protocols / edited by Mihaela Gadjeva, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Harvard University, Boston, MA, USA

Published New York : Humana Press, [2014] ©2014

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Description 1 online resource (xvi, 383 pages) : illustrations (some color) Series Methods in Molecular Biology, 1940-6029 ; 1100 Methods in molecular biology (Clifton, N.J.) ; v. 1100. 1064-3745 Contents Evaluation of Complement Function by ELISA -- Classical Complement Pathway Component C1q? Purification of Human C1q, Isolation of C1q Collagen-like and Globular Head Fragments and Production of Recombinant C1q? Derivatives. Functional Characterization -- Classical Complement Pathway Components C1r and C1s? Purification from Human Serum and in Recombinant Form and Functional Characterization -- Expression and Purification Methods for the Production of Recombinant Human Complement Component C2 -- Purification and Characterisation of human and Mouse Complement C3 -- Purification of Human Complement Protein C5 -- Quantification of Complement C5b-9 Binding to Cells by Flow Cytometry -- Challenges and Opportunities in Fractionation of Recombinant Human Mannan-binding Lectin -- Genotyping of FCN and MBL2 Polymorphisms using Pyrosequencing -- Assay for Estimation of the Functional Activity of the Mannan Binding Lectin Pathway of the Complement System -- Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins -- Purification and Functional Analysis of Human Properdin -- Purification and Functional Characterization of C4b-binding Protein (C4BP) -- Purification and Functional Characterization of Factor I -- C1 inhibitor? Quantification and Purification -- Purification, Quantification and Functional Analysis of Complement Factor H -- Factor H-related Proteins -- Functional Evaluation of Factor H genetic and Acquired Abnormalities; Application for Atypical Hemolytic Uremic Syndrome (aHUS) -- Anti-Factor H Auto Antibodies Assay -- Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage -- Purification of Surfactant Protein D (SP- D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage -- Functional Analysis of C5a Effector Responses In Vitro and In Vivo -- Detection of Complement Receptors 1 and 2 on Mouse Splenic B Cells using Flow Cytometry -- Quantification of Complement Receptor 2 Calcium Signaling Enhancement Using Flow Cytometry -- Purification of C1q Receptors and Functional Analysis -- Detection of Cell Membrane-bound CD46 using Flow Cytometry -- Detection of CD59 using Flow Cytometry -- Functional Analysis of CD59 using Complement Dependent Cytotoxicity Assay -- Detection of Soluble CR3 (CD11b/CD18) by Time-resolved Immunofluorometry -- Analysis of the Complement Activation in Mice -- Opsonophagocytic Assay Summary Complement Systems: Methods and Protocols is composed of 32 individual chapters that describe a variety of protocols to purify and analyze the activity of the individual complement components or pathways. It includes assays that describe detection of complement SNPs, clinical methods to evaluate complement system activation and data interpretation. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Complement Systems: Methods and Protocols provides a collection of well-established classical assays and recently developed new assays to analyze the complement system activation will be useful to a wide audience of scientists Bibliography Includes bibliographical references and index Notes English Online resource; title from PDF title page (SpringerLink, viewed November 18, 2013) Subject Complement (Immunology) -- Laboratory manuals Immune system. Blood proteins. Cell receptors. Membrane proteins. Proteins. Complement (Immunology) Complement activation. Immunoproteins Publication Formats Immune System Phenomena Receptors, Immunologic Blood Proteins Receptors, Cell Surface Publication Characteristics Membrane Proteins Phenomena and Processes Proteins Amino Acids, Peptides, and Proteins Chemicals and Drugs Complement System Proteins Receptors, Complement Laboratory Manuals Complement Activation Immune System protein. Proteins Membrane proteins Immune system Complement activation Cell receptors Blood proteins Complement (Immunology) Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Gadjeva, Mihaela, editor ISBN 9781627037242 1627037241 1627037233 9781627037235

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