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Book Cover

Title In situ hybridization methods / edited by Giselbert Hauptmann
Published New York, NY : Humana Press : Springer, ©2015


Description 1 online resource (xx, 592 pages) : illustrations (some color)
Series Neuromethods, 0893-2336 ; 99
Neuromethods ; 99. 0893-2336
Contents Optimization of in situ hybridization and co-expression analysis in the central nervous system / Louisa Mezache [and others] -- In situ hybridization methods for RNA visualization in C. elegans / Gina Broitman-Maduro and Morris F. Maduro -- Multicolored visualization of transcript distributions in Drosophila embryos / Iris Söll and Giselbert Hauptmann -- Exploring brain genoarchitecture by single and double chromogenic in situ hybridization (ISH) and immunohistochemistry (IHC) in whole-mount embryos / José Luis [and others] -- Exploring brain genoarchitecture by single and double chromogenic in situ hybridization (ISH) and immunohistochemistry (IHC) on cryostat, paraffin, or floating sections / José Luis Ferran [and others] -- Combined radioactive and nonradioactive double in situ hybridization (DISH) / Jong-Hyun Son and Ursula H. Winzer-Serhan -- One FISH, dFISH, three FISH : sensitive methods of whole- mount fluorescent in situ hybridization in freshwater planarians / David D.R. Brown and Bret J. Pearson -- Whole-mount in situ hybridization and immunohistochemistry in xenopus embryos / Edgar M. Pera [and others] -- Manual and automated whole-mount in situ hybridization for systematic gene expression analysis in embryonic Zebrafish forebrain / Iris Söll and Giselbert Hauptmann -- Large-scale ISH on mouse brain sections for systematic gene expression analysis in developing mouse diencephalon / Tomomi Shimogori and Seth Blackshaw -- High-throughput in situ hybridization : systematical production of gene expression data and beyond / Lars Geffers and Gregor Eichele -- Analysis of embryonic gene expression patterns in the hagfish / Yasuhiro Oisi [and others] -- Gene expression analysis of lamprey embryos / Fumiaki Sugahara, Yasunori Murakami, and Shigeru Kuratani -- Detection of mRNA and microRNA expression in basal chordates, amphioxus and ascidians / Simona Candiani, Greta Garbarino, and Mario Pestarino -- Analysis of gene expression in neurons and synapses by multi-color in situ hybridization / Leonid L. Moroz and Andrea B. Kohn -- Fluorescent in situ hybridization in primary hippocampal neurons to detect localized mRNAs / Jacki E. Heraud-Farlow, Tejaswini Sharangdhar, and Michael A. Kiebler -- Combining whole-mount in situ hybridization with neuronal tracing and immunohistochemistry / Jeremy S. Duncan [and others] -- Fluorescent in situ hybridization detection of microRNAs in Newt tissue cryosections / Jamie Ian Morrison -- Subcellular transcript localization in Drosophila embryos and tissues visualized by multiplex-FISH / Julie Bergalet [and others] -- Application of alkaline phosphatase-mediated azo dye staining for dual fluorescent in situ hybridization in Zebrafish / Giselbert Hauptmann, Gilbert Lauter, and Iris Söll -- Multiplex fluorescent RNA in situ hybridization via RNAscope / Hongwei Wang [and others] -- Quantum dot-based in situ hybridization and immunohistochemistry to detect mRNA and protein at subcellular levels, comparison with studies using electron microscopy / Akira Matsuno [and others] -- Quantitative multiplexed quantum dot based in situ hybridization in formalin-fixed paraffin-embedded tissue / Eleni Tholouli, Judith A. Hoyland, and Richard J. Byers -- Single and multiplexed fluorescent in situ hybridization using quantum dots nanocrystals in xenopus / Andriani Ioannou [and others] -- Quantitative single-molecule mRNA fluorescent in situ hybridization in C. elegans / Remco A. Mentink [and others] -- In situ detection of individual mRNA molecules with padlock probes and target-primed rolling-circle amplification in fixed mouse brain tissues / Thomas Hauling [and others] -- Extended multiplexed fluorescent in situ hybridization by combinatorial encoding of individual transcripts / Lars Martin Jakt and Satoko Moriwaki -- Characterizing cellular identity at one cell resolution / Amaresh K. Ranjan, Mugdha V. Joglekar, and Anandwardhan A. Hardikar -- Transglutaminase-mediated in situ hybridization (TransISH) for mRNA detection in mammalian tissues / Katsuyuki Miyawaki, Sumihare Noji, and Noriho Kamiya -- ECHO-FISH for gene transcript detection in neuronal and other cells and subcellular compartments / Dan Ohtan Wang and Akimitsu Okamoto
Summary Annotation This volume" "contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular "Neuromethods"series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, "In Situ Hybridization Methods"provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications."
Analysis biomedische wetenschappen
Medicine (General)
Geneeskunde (algemeen)
Bibliography Includes bibliographical references and index
Notes English
Online resource; title from PDF title page (SpringerLink, viewed February 23, 2015)
Subject In situ hybridization -- Laboratory manuals
Nucleic acid hybridization.
Stains and staining (Microscopy)
Genetics -- Technique.
Cytology -- Technique.
Histology -- Technique.
Medical laboratory technology.
In situ hybridization.
Nucleic Acid Hybridization
Staining and Labeling
Investigative Techniques
Histocytological Preparation Techniques
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Genetic Techniques
Cytological Techniques
Histological Techniques
Clinical Laboratory Techniques
In Situ Hybridization
staining (testing technique)
stains (damage)
stain (coating)
Medical -- Neuroscience.
Science -- Life Sciences -- Zoology -- General.
Stains and staining (Microscopy)
Nucleic acid hybridization
Medical laboratory technology
Histology -- Technique
Genetics -- Technique
Cytology -- Technique
In situ hybridization
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Hauptmann, Giselbert, editor
ISBN 9781493923038