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Title Immunocytochemistry and related techniques / edited by Adalberto Merighi and Laura Lossi
Published New York, NY : Humana Press : Springer, ©2015


Description 1 online resource (xiii, 473 pages) : illustrations (some color)
Series Neuromethods, 0893-2336 ; 101
Neuromethods ; 101. 0893-2336
Contents 880-01 Machine generated contents note: 1. The Evolution of Immunocytochemistry in the Dissection of Neural Complexity / Laura Lossi -- pt. I Immunocytochemistry in Non-Mammalian Brain -- 2. Immunofluorescence and Genetic Fluorescent Labeling Techniques in the Drosophila Nervous System / Christian Wegener -- 3. Immunohistochemical Analysis of Neuronal Networks in the Nervous System of Octopus vulgaris / Graziano Fiorito -- 4. Genetic Mosaic Labeling and Immunofluorescence Techniques in Zebrafish Brain / Yoshihiro Yoshihara -- pt. II Detection of Cell Proliferation and Neurogenesis -- 5. Immunocytochemical Phenotype of Differentiating Neurons / Antonio Carai -- 6. Immunocytochemical Identification of Mammalian Differentiating Neurons in the Process of Adult Neurogenesis in the Hippocampal Dentate Gyrus / Oliver von Bohlen und Halbach -- 7. Identification of Proliferating and Migrating Cells by BrdU and Other Thymidine Analogs: Benefits and Limitations / Pasko Rakic
880-01/(S Evolution of immunocytochemistry in the dissection of neural complexity / Adalberto Merighi and Laura Lossi -- Immunofluorescence and genetic fluorescent labeling techniques in the Drosophila nervous system / Mareike Selcho and Christian Wegener -- Immunohistochemical analysis of neuronal networks in the nervous system of Octopus vulgaris / Giovanna Ponte and Graziano Fiorito -- Genetic mosaic labeling and immunofluorescence techniques in Zebrafish brain / Nobuhiko Miyasaka, Noriko Wakisaka, and Yoshihiro Yoshihara -- Immunocytochemical phenotype of differentiating neurons / Andrea Diana and Antonio Carai -- Immunocytochemical identification of mammalian differentiating neurons in the process of adult neurogenesis in the hippocampal dentate gyrus / Oliver von Bohlen und Halbach -- Identification of proliferating and migrating cells by BrdU and other thymidine analogs : benefits and limitations / Alvaro Duque and Pasko Rakic -- Flow cytometry-based quantification of neurogenesis in the central nervous system / Armin Schneider -- In vivo and in vitro immunohistochemical visualization of neural cell apoptosis and autophagy / Maria Grazia Bottone, Francesco Paolo Fanizzi, and Graziella Bernocchi -- Immunocytochemical detection of intraneuronal Aβ peptides in mouse models of Alzheimer's disease / Oliver Wirths and Anika Saul -- Purification and characterization of mouse olfactory ensheathing cells and their potential use for therapy in amyotrophic lateral sclerosis (ALS) / Chrystian Junqueira Alves [and others] -- Immunocytochemistry of microglial cells / Dmitrii E. Korzhevskii, Olga Kirik, and Elena Sukhorukova -- Immunocytochemical assessment of blood-brain barrier structure, function, and damage / Jean-Pierre Louboutin -- Quantification of immunocytochemical colocalization in neurons / Brad R. Rocco and Kenneth N. Fish -- Immunogold electron microscopic quantification of small molecular compounds and proteins at synapses and other neural profiles / Max Larsson, Linda Hildegard Bergersen, and Vidar Gundersen -- Combined immunocytochemistry and tracing of neural connections / Taisuke Miyazaki and Masahiko Watanabe -- Neuronal circuitry dissected by immunocytochemistry combined with retrograde tracing and electrophysiology / Alberto Granato and Andrea De Giorgio -- Transfection techniques and combined immunocytochemistry in cell cultures and organotypic slices / Silvia Alasia, Adalberto Merighi, and Laura Lossi -- Combined immunochemistry and live imaging of fluorescent protein expressing neurons in mouse brain / Ruth M. Empson [and others] -- Array tomography : a novel high-resolution immunofluorescence technique / Mariano Soiza-Reilly -- Single molecule localization microscopy to study neuronal microtubule organization / Bas M.C. Cloin [and others] -- Studying membrane dynamics of proteins using quantum dots / Martin Heubl and Sabine Lévi -- Optical protein patterning / Santiago Costantino
Summary Annotation This volume presents a collection of protocols for immunocytochemical analysis of neurons and neural networks. Chapters focus on immunocytochemical localization at light and electronic levels, biochemical characterization, and functional analysis in vivo or ex vivo by novel types of microscopy, as well as protocols for development and production of genetic probes. Written for the popular Neuromethodsseries, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, Immunocytochemistry and Related Techniques is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, electrophysiologists that are currently active in the field or are willing to enter the exciting area of neuroscience research
Analysis biomedische wetenschappen
Medicine (General)
Geneeskunde (algemeen)
Bibliography Includes bibliographical references and index
Notes English
Online resource; title from PDF title page (SpringerLink, viewed March 3, 2015)
Subject Immunocytochemistry -- Laboratory manuals
Cell physiology.
Cell Physiological Phenomena
Immunohistochemistry -- methods
Chemistry Techniques, Analytical -- methods
Histocytological Preparation Techniques -- methods
Medical -- Neuroscience.
Cell physiology
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Merighi, Adalberto, editor.
Lossi, Laura, editor
ISBN 9781493923137