| Description |
1 online resource (xvi, 397 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 1628 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1628. 1064-3745
|
| Contents |
Marburg- and ebolaviruses : a look back and lessons for the future / Hans Dieter Klenk and Werner Slenczka -- Forty years of ebolavirus molecular biology : understanding a novel fisease agent through the development and application of new technologies / Allison Groseth and Thomas Hoenen -- Ebolavirus : an overview of molecular and clinical pathogenesis / Veronica Vine, Dana P. Scott, and Heinz Feldmann -- Production of filovirus glycoprotein-pseudotyped vesicular stomatitis virus for study of filovirus entry mechanisms / Rachel B. Brouillette and Wendy Maury -- Lentiviral vectors pseudotyped with filoviral glycoproteins / Patrick L. Sinn, Jeremy E. Coffin, Natarajan Ayithan, Kathleen H. Holt, and Wendy Maury -- Modeling eEbola virus genome replication and transcription with minigenome systems / Tessa Cressey, Kristina Brauburger, and Elke Mühlberger -- Quantification of RNA content in reconstituted ebola virus nucleocapsids by immunoprecipitation / Logan Banadyga and Hideki Ebihara -- Modeling ebolavirus budding with virus like particles / Olivier Reynard and Mathieu Mateo -- Modeling the ebolavirus life cycle with transcription and replication-competent viruslike particle assays / Nadine Biedenkopf and Thomas Hoenen -- Assays to measure suppression of type I interferon responses by filovirus VP35 proteins / Priya Luthra and Christopher F. Basler -- Nonradioactive northern blot analysis to detect ebola virus minigenomic mRNA / Kristina Brauburger, Tessa Cressey, and Elke Mühlberger -- Semi-automated high-throughput microtitration assay for filoviruses / Claire Marie Filone, David Miller, and Victoria Wahl-Jensen -- Generation of recombinant ebola viruses using reverse genetics / Allison Groseth -- Luciferase-expressing ebolaviruses as tools for screening of antivirals / Thomas Hoenen -- Live-cell imaging of filoviruses / Gordian Schudt, Olga Dolnik, and Stephan Becker -- Assessment of inhibition of ebola virus progeny production by antiviral compounds / Darryl Falzarano -- Analysis of the cellular stress response during ebola virus infection by immunofluorescence / Emily V. Nelson and Kristina M. Schmidt -- Analyzing apoptosis induction and evasion in ebola virus-infected cells / Judith Olejnik and Emily V. Nelson -- Electron microscopy of ebola virus-infected cells / Takeshi Noda -- Validating the inactivation effectiveness of chemicals on ebola virus / Elaine Haddock and Friederike Feldmann -- Visualizing ebolavirus particles using single-particle interferometric reflectance imaging sensor (SP-IRIS) / Erik P. Carter, Elif Ç. Seymour, Steven M. Scherr, George G. Daaboul, David S. Freedman, M. Selim Ünlü, and John H. Connor -- Assessing antiviral countermeasures using mouse models of ebolavirus infection / Andrea Kroeker, Bryan D. Griffin, Xiangguo Qiu, and Gary Kobinger -- Evaluation of ebola virus countermeasures in guinea pigs / Andrea Marzi -- Evaluation of medical countermeasures against ebolaviruses in nonhuman primate models / Chad E. Mire and Thomas W. Geisbert -- Quantification of filovirus glycoprotein-specific antibodies / Wakako Furuyama, Hiroko Miyamoto, Reiko Yoshida, and Ayato Takada -- Monitoring innate immune gene responses in the hamster model of ebola virus disease by RT-PCR / Marko Zivcec -- Real-time and end-point PCR diagnostics for ebola virus / Allen Grolla -- Production of antigens for ELISA / Robert W. Cross and Thomas G. Ksiazek -- ELISA methods for the detection of ebolavirus infection / Robert W. Cross and Thomas G. Ksiazek -- Ebola virus field sample collection / Brian R. Amman, Amy J. Schuh, and Jonathan S. Towner |
| Summary |
This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology. After an introduction, the volume delves into protocols for studying ebolavirus molecular biology under biosafety level 2 conditions, studying infectious ebolaviruses under biosafety level 4 conditions in vitro as well as in vivo, and working with ebolaviruses in the field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerProtocols, viewed June 2, 2017) |
| Subject |
Ebola virus disease -- Laboratory manuals
|
|
Ebola virus disease.
|
|
Ebolavirus
|
|
Hemorrhagic Fever, Ebola
|
|
Infectious & contagious diseases.
|
|
Medical -- Infectious Diseases.
|
|
Ebola virus disease
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Hoenen, Thomas, editor
|
|
Groseth, Allison, editor
|
| ISBN |
9781493971169 |
|
1493971166 |
|
