| Description |
1 online resource (1 PDF file (xi, 260 pages)) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 2196 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; v. 2196. 1064-3745
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Springer protocols (Series) 1949-2448
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| Contents |
1. Use of Yeast Plasmids -- Transformation and Inheritance AssaysAnastasiia Mereshchuk, Joyce S.K. Chew, and Melanie J. Dobson 2. Construction of Tight Conditional Mutants Using the Improved Auxin-inducible Degron (iAID) Method in the Budding Yeast Saccharomyces cerevisiaeSeiji Tanaka 3. CRISPR Nickase-Mediated Base Editing in YeastKouichi Kuroda and Mitsuyoshi Ueda 4. Genomic Promoter Shuffling by Using Recyclable Cassettes Xuelei Tian, Wenqing Zhang, and Wei Xiao 5. Study Essential Gene Functions by Plasmid ShufflingLi Fan and Wei Xiao 6. Scarless Genomic Protein Labeling in Saccharomyces cerevisiaeQian Wang, Yu V. Fu, and Wei Xiao 7. High-Copy Yeast Library Construction and High-Copy Rescue Genetic Screen in Saccharomyces cerevisiae Fanli Zeng and David G. Quintana 8. Genome-Wide Imaging-Based Phenomic Screening Using Yeast (Saccharomyces cerevisiae) Strain CollectionsXiuling Cao, Xuejiao Jin, and Beidong Liu 9. Applications of Oxford Nanopore Sequencing in Schizosaccharomyces pombe Ming He, Xu Chi, and Jie Ren 10. Measuring Genome-Wide Nascent Nucleosome Assembly Using ReIN-MapZhiyun Xu, Jianxun Feng, and Qing Li 11. Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate AssemblyYixuan Zhu and Limin Cao 12. Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLEZhouqing Luo, Shuangying Jiang, and Junbiao Dai 13. Use YeastFab to Construct Genetic Parts and Multicomponent Pathways for Metabolic EngineeringShuangying Jiang, Zhouqing Luo, and Junbiao Dai 14. A Versatile Protocol to Generate Translocations in Yeast Genomes using CRISPR/Cas9Nicolas Agier, Aubin Fleiss, Stéphane Delmas, and Gilles Fischer15. Yeast Nucleoplasmic Extracts and an Application to Visualize Chromatin Assembly on Single Molecules of DNA Yong Wang and Yu V. Fu 16. Assays for Autophagy III: Observing Dynamic Protein TraffickingJing Zhu and Zhiping Xie 17. Isolation of Aged Yeast Cells Using Biotin-Streptavidin Affinity PurificationXuejiao Jin, Xiuling Cao, and Beidong Liu 18. High-Throughput Rapid Yeast Chronological Lifespan AssayAbd-Al-Wahab A. Khawaja, Zach R. Belak, Christopher H. Eskiw, and Troy A. A. Harkness 19. Long-Term Imaging and Dynamic Analysis of Cytoophidia in YeastShanshan Zhang, Hui Li, and Ji-Long Liu 20. Monitoring 5'-end Resection at Site-specific Double-strand Breaks by Southern Blot AnalysisHaoyang Peng, Simin Zhang, and Xuefeng Chen |
| Summary |
This fourth edition provides all new methods on yeast that have not been included in the past three editions. Divided in four parts, chapters guide readers through general applications for genetic manipulation in yeast cells, genome-wide studies, systematically rearrange the yeast chromosomes, and pathway methods. Written in the Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed October 21, 2020) |
| Subject |
Yeast fungi -- Biotechnology.
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Yeast fungi -- Research -- Methodology
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Yeasts -- physiology
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Yeasts -- cytology
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Yeasts -- isolation & purification
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Yeast fungi -- Biotechnology
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Xiao, Wei, 1957 April 10- editor.
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| ISBN |
9781071608685 |
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1071608681 |
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