Title Fusarium wilt : methods and protocols / edited by Jeffrey Coleman

Published New York, NY : Humana Press, [2022]

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Description 1 online resource (1 PDF file (xi, 208 pages)) : illustrations (some color) Series Methods in molecular biology, 1940-6029 ; volume 2391 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 2391. 1064-3745 Springer protocols (Series) 1949-2448 Contents Pure culture and DNA sequence-based identification of Fusarium from symptomatic plants and diverse substrates -- Isolation of high-molecular weight (HMW) DNA from Fusarium oxysporum for long-read sequencing -- Karyotyping of Fusarium oxysporum by pulsed field gel electrophoresis and the germ tube burst method -- RNA-seq data analysis for differential expression -- Quantification and isolation of spontaneous colony growth variants -- Generation of a T-DNA insertion mutant library of Fusarium oxysporum using Agrobacterium tumefaciens -- Targeted gene disruption via CRISPR/Cas9 ribonucleoprotein complexes in Fusarium oxysporum -- CRISPR/Cas9 RNP-mediated gene fusion to assess protein quantification and subcellular localization in Fusarium oxysporum -- In vivo monitoring of cytosolic pH using the ratiometric pH sensor pHluorin -- Multi-pronged investigation of volatile compound-mediated interactions of Fusarium oxysporum with plants, fungi and bacteria -- Isolation of mitochondria from Fusarium species -- In planta gene expression analysis and colonization of Fusarium oxysporum -- Time-lapse imaging of root pathogenesis and fungal proliferation without physically disrupting roots -- High-throughput screening assays to identify plant natural products with antifungal properties against Fusarium oxysporum -- Screening and assessment of pisatin demethylase activity (PDA).-Detection and genotyping of Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), the Fusarium wilt pathogen of cotton Summary This volume provides a collection of molecular protocols detailing the most common and modern techniques on fusarium wilt. Chapters guide readers through methods on initial isolation, molecular-based identification, genome characterization, generation of mutants, and characterization of interactions with other organisms including host plants. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Fusarium wilt: Methods and Protocols aims to be a valuable resource for mycologists, plant pathologists, microbiologists, geneticists, and other scientists that have an interest in members of the Fusarium oxysporum species complex or closely related fungi Bibliography Includes bibliographical references and index Notes Description based on online resource; title from PDF title page (SpringerLink, viewed June 10, 2022) Subject Fusarium diseases of plants -- Laboratory manuals Fusarium oxysporum -- Laboratory manuals Fusarium. Botany. Microbial genetics. Fusarium Plant Diseases -- parasitology Genetics, Microbial Botany botany. Microbial genetics Fusarium Botany Fusarium diseases of plants Fusarium oxysporum Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Coleman, Jeffrey (Assistant professor), editor. ISBN 9781071617953 1071617958

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