| Description |
1 online resource (176 p.) |
| Series |
River Publishers Series in Biotechnology and Medical Research Series |
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River Publishers series in biotechnology and medical research
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| Contents |
Cover -- Half Title -- Series Page -- Title Page -- Copyright Page -- Table of Contents -- Preface -- List of Contributors -- List of Figures -- List of Abbreviations -- Introduction: Core Techniques in Stem Cell Culture -- Introduction -- Adult Stem Cell -- Fetal Stem Cell -- Cancer Stem Cell (CSC) -- Other Supporting Methods -- References -- Chapter 1: Isolation of Hematopoietic Stem Cells -- 1.1: Introduction -- 1.2: Materials -- 1.3: Method -- 1.4: Notes -- References |
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Chapter 2: Isolation Protocol for CD133+ and CD34+ Very Small Embryonic-like Cells (VSEL) from Human Umbilical Cord Blood -- 2.1: Introduction -- 2.2: Materials -- 2.2.1: Human umbilical cord blood (hUCB) -- 2.2.2: Reagents for hUCB CD34+ and CD133+ VSEL isolation -- 2.2.3: Antibodies -- 2.2.4: Plastics and equipment -- 2.3: Methods -- 2.3.1: Isolation of MNCs -- 2.3.2: Staining for CD133+ or CD34+ VSELs -- 2.3.3: Sorting protocol -- 2.4: Notes -- References -- Chapter 3: Isolation and Expansion of Mesenchymal Stem Cells from the Adult Bone Marrow -- 3.1: Introduction -- 3.2: Materials |
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3.3: Methods -- 3.3.1: Bone marrow collection (also Method 13) -- 3.3.2: Plating bone marrow aspirates -- 3.3.3: Red blood cell removal -- 3.3.4: Culturing of MSCs -- 3.4: Notes -- References -- Chapter 4: Manual Isolation and Expansion of Adipose-derived Stem Cells from Adipose Tissue -- 4.1: Introduction -- 4.2: Materials -- 4.3: Methods -- 4.3.1: Preparation -- 4.3.2: Lipoaspirate preparation -- 4.3.3: Block adipose preparation -- 4.3.4: Enzymatic digestion of adipose tissue (optional) -- 4.3.5: ASC culture and expansion -- 4.4: Notes -- References |
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Chapter 5: Isolating Compact Bone-derived Mesenchymal Stem Cells from Rodent and Rabbit Femurs -- 5.1: Introduction -- 5.2: Materials -- 5.2.1: Reagents for CBM-MSC isolation -- 5.2.2: Animals -- 5.2.3: Materials -- 5.3: Methods -- 5.3.1: Isolating CBM MSCs -- 5.4: Notes -- References -- Chapter 6: Isolating Dental Pulp Stem Cells -- 6.1: Introduction -- 6.2: Materials -- 6.2.2: Dental instruments -- 6.2.3: DPSCs culture media -- 6.2.4: Flow cytometry for phenotype -- 6.3: Methods -- 6.3.1: Tooth preparation -- 6.3.2: Dental pulp extraction -- 6.3.3: Dental pulp preparation -- 6.4: Notes |
| Summary |
This volume on stepwise techniques to culture adult human stem cells begins with the basic methods to culture adult stem cells followed by related methods to supplement stem cell culture. This and future volumes of techniques will allow laboratories across scientific entities - academic and commercial - to adapt the described methods for similar methods with cells from other species. The standardized techniques will allow across the board interpretation of other technologies such as Omics studies. These methods can be a starting platform for culturing stem cells even in laboratories that might not be familiar with the techniques. Scientific and early clinical trials strongly favor the development of translational studies with adult stem cells. A major impediment to progress in the field of regenerative medicine is a lack of standardized methods to culture specific stem cells. Such documented methods would allow for scientific rigor and reproducibility to enhance efficient and safe translation of stem cells to patients. This book fills that gap |
| Notes |
Description based upon print version of record |
| Bibliography |
References -- Chapter 7: A Murine System for Somatic Cellular Reprogramming into Induced Pluripotent Stem Cells -- 7.1: Introduction -- 7.2: Material -- 7.2.1: Reprogrammable mouse model and reagents to prepare murine embryonic fibroblasts (MEFs) -- 7.2.2: Preparation of 2i/LIF media supplemented with vitamin C -- 7.2.3: Quantification of iPSC colonies by alkaline phosphatase (AP) staining -- 7.3: Method -- 7.3.1: Harvesting somatic cells from reprogrammable mouse model (see Notes 1 and 2) -- 7.3.2: Somatic cell reprogramming into iPSCs using 2i/LIF media supplemented with Vitamin C -- 7.4: Notes |
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References |
| Notes |
Pranela Rameshwar is a tenured professor of medicine, Division of Hematology and Oncology at Rutgers New Jersey Medical School, Rutgers. She received her B.Sc. degree in medical microbiology from the University of Wisconsin at Madison and Ph.D. in biology from Rutgers University, New Jersey. She performed postdoctoral studies in hematopoiesis at New Jersey Medical School. Dr. Rameshwar's research interest in stem cell biology began with her doctoral thesis and continues with research on breast cancer dormancy, neural regulation of hematopoiesis, and clinical application of adult human mesenchymal stem cells. Her research continues to be funded by federal, state, and other agencies. Dr. Rameshwar has edited books and authored >200 publications, which include original articles, reviews, editorials and book chapters. Dr. Rameshwar initiated a certificate program in stem cell biology and directs four graduate level courses in stem cell biology |
| Subject |
Adult stem cells -- Laboratory manuals
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Human cell culture -- Laboratory manuals
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SCIENCE / Life Sciences / Biology / General
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| Form |
Electronic book
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| ISBN |
9781040010778 |
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1040010776 |
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9781003466871 |
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1003466877 |
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9781040010808 |
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1040010806 |
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