Title Nonviral vectors for gene therapy : methods and protocols / edited by Mark A. Findeis

Published Totowa, N.J. : Humana Press, ©2001

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Description 1 online resource (xi, 399 pages) : illustrations Series Methods in molecular medicine ; 65 Methods in molecular medicine ; 65. 1543-1894 Contents Nonviral Vectors for Gene Therapy -- Preface -- Contents -- Contributors -- 1 Synthesis of Polyampholyte Comb-Type Copolymers Consisting of Poly(L-lysine) Backbone and Hyaluronic Acid Side Chains for DNA Carrier -- 2 Cationic alpha-Helical Peptides for Gene Delivery into Cells -- 3 Supramolecular Self-Assembly of Poly(ethylene glycol)-Block-Poly(L-lysine) Dendrimer with Plasmid DNA -- 4 Water-Soluble Cationic Methacrylate Polymers for Nonviral Gene Delivery -- 5 Stabilization of Polycation�DNA Complexes by Surface Modification with Hydrophilic Polymers 6 Use of Disulfide Cationic Lipids in Plasmid DNA Delivery7 Interactions of Lipid�Oligonucleotide Conjugates with Low-Density Lipoprotein -- 8 Coupling of Nuclear Localization Signals to Plasmid DNA -- 9 Progress Toward a Synthetic Virus: A Multicomponent System for Liver-Directed DNA Delivery -- 10 Characterization of Polyampholyte Comb-Type Copolymer DNA Carriers -- 11 Methods for Studying Formation of Polycation�DNA Complexes and Properties Useful for Gene Delivery -- 12 Characterization of DNA Condensates by Atomic Force Microscopy 13 Rapid and Systematic Transfer and Recovery of Large BACs/PACs into Mammalian Cells by HAEC Retrofitting14 Systemic Delivery of Therapeutic Proteins by Intramuscular Injection of Plasmid DNA -- 15 Local Delivery of Therapeutic Proteins by Intratumoral Injection of Plasmid DNA�Lipid Complexes -- 16 Nonviral DNA Delivery from Polymeric Systems -- 17 Promotion of Duplex and Triplex DNA Formation by Polycation Comb-Type Copolymers -- 18 Lyophilization of Nonviral Gene Delivery Systems 19 Ultraviolet Absorption and Circular Dichroism Spectroscopy of Nonviral Gene Delivery Complexes20 Characterization of Synthetic Gene Delivery Vectors by Infrared Spectroscopy -- 21 Characterization of Cationic Vector-Based Gene Delivery Vehicles Using Isothermal Titration and Differential Scanning Calorimetry -- 22 Light-Scattering Techniques for Characterization of Synthetic Gene Therapy Vectors -- 23 Renal Gene Therapy -- Index Summary Nonviral gene therapy-an emerging field with great clinical promise-avoids many problems associated with viral gene therapy and offers the possibility of superior clinical effectiveness. In Nonviral Vectors for Gene Therapy: Methods and Protocols, Mark A. Findeis has assembled a panel of active researchers to present their best methods not only for preparing, handling, and characterizing gene delivery agents, but also for gene delivery. To help those preparing and characterizing gene transfer agents, the contributors examine a broad range of compounds that bind with DNA to form the compact complexes that facilitate cellular delivery-among them peptide conjugates, synthetic polymers, and lipids. They also outline specific approaches to gene transfer in vivo, including direct delivery by intratumoral injection and indirect delivery by cell-specific targeting of DNA complexes, and discuss in detail many spectroscopic techniques for characterizing nonviral gene delivery agents. Opportunities for the development and application of these novel vectors in the research lab and eventually, in the clinic, are highlighted. Comprehensive and state-of-the-art, Nonviral Vectors for Gene Therapy: Methods and Protocols illuminates for today's investigators the powerful new approaches to the creation of nonviral materials, as well as their efficacious use in delivering therapeutic genes to patients across the spectrum of human disease Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English Print version record digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL In Springer Protocols Subject Gene therapy -- Laboratory manuals Genetic vectors -- Laboratory manuals Biological products -- Therapeutic use. Genetics -- Technique. Genetic engineering. Chemotherapy. Therapeutics. Gene therapy. Methodology. Genetic vectors. Investigative Techniques Biological Therapy Genetic Structures Genetic Techniques Genetic Engineering Drug Therapy Therapeutics Analytical, Diagnostic and Therapeutic Techniques and Equipment Genetic Phenomena Phenomena and Processes Genetic Therapy Methods Drug Delivery Systems Gene Transfer Techniques Genetic Vectors methodology. treating (health care function) HEALTH & FITNESS -- Diseases -- Genetic. MEDICAL -- Genetics. Therapeutics Methodology Genetics -- Technique Genetic engineering Chemotherapy Biological products -- Therapeutic use Gene therapy Genetic vectors Gentherapie. Wetenschappelijke technieken. Genre/Form Laboratory manuals Form Electronic book Author Findeis, Mark A. ISBN 9781592591398 1592591396 0896037126 9780896037120

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