| Description |
1 online resource (xi, 355 pages) : illustrations (chiefly color) |
| Series |
Methods in molecular biology, 1940-6029 ; 1521 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1521. 1064-3745
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| Contents |
Current methods in cardiac gene therapy : overview / Kiyotake Ishikawa and Roger J. Hajjar -- Silencing genes in the heart / Henry Fechner, Roland Vetter, Jens Kurreck, and Wolfgang Poller -- Generation of efficient miRNA inhibitors using tough decoy constructs / Jimeen Yoo, Roger J. Hajjar, and Dongtak Jeong -- Efficient genome editing in induced pluripotent stem cells with engineered nucleases in vitro / Vittavat Termglinchan, Timon Seeger, Caressa Chen, Joseph C. Wu, and Ioannis Karakikes -- Direct cardiac reprogramming as a novel therapeutic strategy for treatment of myocardial infarction / Hong Ma, Li Wang, Jiandong Liu, and Li Qian -- Production and characterization of vectors based on the cardiotropic AAV serotype 9 / Erik Kohlbrenner and Thomas Weber -- Cell-based measurement of neutralizing antibodies against adeno-associated virus (AAV) / Andreas Jungmann, Oliver Müller, and Kleopatra Rapti -- Synthesis of modified mRNA for myocardial delivery / Jason Kondrat, Nishat Sultana, and Lior Zangi -- Exosomes-based gene therapy for microRNA delivery / Prabhu Mathiyalagan and Susmita Sahoo -- Lipidoid mRNA nanoparticles for myocardial delivery in rodents / Irene C. Turnbull, Ahmed A. Eltoukhy, Daniel G. Anderson, and Kevin D. Costa -- Gene transfer in isolated adult cardiomyocytes / Kjetil Hodne, David B. Lipsett, and William E. Louch -- Gene transfer in cardiomyocytes derived from ES and iPS cells / Francesca Stillitano, Ioannis Karakikes, and Roger J. Hajjar -- Gene transfer to rodent hearts in vivo / Federica del Monte, Kiyotake Ishikawa, and Roger J. Hajjar -- Ultrasound-targeted microbubble destruction for cardiac gene delivery / Shuyuan Chen and Paul A. Grayburn -- Needleless liquid jet injection delivery approach for cardiac gene therapy / Anthony S. Fargnoli, Michael G. Katz, and Charles R. Bridges -- Cardiac gene delivery in large animal models : antegrade techniques / Shin Watanabe, Lauren Leonardson, Roger J. Hajjar, and Kiyotake Ishikawa -- Direct myocardial injection of vectors / Guillaume Bonnet, Kiyotake Ishikawa, Roger J. Hajjar, and Yoshiaki Kawase -- Selective pressure-regulated retroinfusion for gene therapy application in ischemic heart disease / Rabea Hinkel and Christian Kupatt -- Cardiac gene delivery using recirculating devices / Melissa J. Byrne and David M. Kaye -- Molecular cardiac surgery with recirculating delivery (MCARD) : procedure and vector transfer / Michael G. Katz, Anthony S. Fargnoli, Andrew P. Kendle, and Charles R. Bridge -- Gene delivery for the generation of bioartificial pacemaker / Patrick K.W. Chan and Ronald A. Li -- Gene therapy for post-infarction ventricular tachycardia / J. Kevin Donahue -- MicroRNA delivery strategies to the lung in a model of pulmonary hypertension / Lin Deng, Andrew H. Baker, and Angela C. Bradshaw -- Inhaled gene transfer for pulmonary circulation / Jaume Aguero, Lahouaria Hadri, Nadjib Hammoudi, Lauren Leonardson, Roger J. Hajjar, and Kiyotake Ishikawa |
| Summary |
This detailed book provides methodological information on cardiac gene delivery, from classic to state-of-the-art technologies and techniques. Efficient, cardiac-specific, and safe vectors, as well as refined vector delivery methods, are key for successful cardiac gene transfer and eventually for improving patients' outcomes. Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Gene Therapy: Methods and Protocols serves as a valuable tool for molecular biologists and physiologists in the cardiology field conducting cardiac gene transfer research, which will ultimately lead to further advancements in the vital field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerProtocol, viewed December 7, 2016) |
| Subject |
Cardiovascular system -- Diseases -- Genetics
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Genetic vectors.
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Heart Diseases -- therapy
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Genetic Therapy -- methods
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Gene Transfer Techniques
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Genetic Vectors
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Medicine
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Molecular biology
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| Genre/Form |
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Ishikawa, Kiyotake, editor
|
| LC no. |
2016959002 |
| ISBN |
9781493965885 |
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1493965883 |
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1493965867 |
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9781493965861 |
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