| Description |
1 online resource (xiv, 392 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 15 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 15
|
| Contents |
Polymerase chain reaction : basic protocols / Beverly C. Delidow, John P. Lynch, John J. Peluso, Bruce A. White -- Selection of primers for polymerase chain reaction / Wojciech Rychlik -- Direct radioactive labeling of polymerase chain reaction products / Tim McDaniel, Stephen J. Meltzer -- Use of arithmetic polymerase chain reaction for synthesis of single-stranded probes for S1 nuclease assays / Puja Agarwal, Bruce A. White -- Nonradioactive labeling of polymerase chain reaction products / Udo Reischl, Rüdiger Rüger, Christoph Kessler -- Quantitation and purification of polymerase chain reaction products by high-performance liquid chromatography / Elena D. Katz -- Use of polymerase chain reaction for screening transgenic mice / Shizhong Chen, Glen A. Evans -- Polymerase chain reaction analysis of DNA from paraffin-embedded tissue / Matthias Volkenandt, Adam P. Dicker, Renato Fanin, Debabrata Banerjee, Anthony Albino, Joseph R. Bertino -- The use of polymerase chain reaction for chromosome assignment / Michael Jaye, Craig A. Dionne -- Mapping MHC class II genes and disease-susceptibility : use of polymerase chain reaction and dot hybridization for human leukocyte antigen allele typing / Stephen C. Bain, John A. Todd -- The use of the polymerase chain reaction and the detection of amplified products / Robert C. Allen, Bruce Budowle -- Determination of loss of heterozygosity using polymerase chain reaction / Stephen J. Meltzer -- Direct sequencing of polymerase chain reaction products / Stephen J. Meltzer -- Manual and automated direct sequencing of product generated by the polymerase chain reaction / Adam P. Dicker, Matthias Volkenandt, Joseph R. Bertino -- Genomic footprinting by ligation mediated polymerase chain reaction / Gerd P. Pfeifer, Arthur D. Riggs -- RNA template-specific polymerase chain reaction (RS-PCR) : a modification of RNA-PCR that dramatically reduces the frequency of false positives / Alan R. Shuldiner, Riccardo Perfetti, Jesse Roth -- Quantitative measurement of relative gene expression in human tumors / Tetsuro Horikoshi, Kathleen Danenberg, Matthias Volkenandt, Thomas Stadlbauer, Peter V. Danenberg -- Identification of alternatively spliced mRNAs and localization of 5' ends by polymerase chain reaction amplification / Cheng-Ming Chiang, Louise T. Chow, Thomas R. Broker -- Utilization of polymerase chain reaction for clonal analysis of gene expression / Alice L. Witsell, Lawrence B. Schook -- Sequencing DNA amplified directly from a bacterial colony / Martin A. Hofmann, David A. Brian -- Use of polymerase chain reaction to screen phage libraries / Lei Yu, Laura J. Bloem -- Molecular cloning of polymerase chain reaction fragments with cohesive ends / Beverly C. Delidow -- Rapid (ligase-free) subcloning of polymerase chain reaction products / Alan R. Shuldiner, Keith Tanner -- Use of polymerase chain reaction for making recombinant constructs / Douglas H. Jones, Stanley C. Winistorfer -- In vitro recombination and mutagenesis of DNA : SOEing together tailor-made genes / Robert M. Horton -- Use of polymerase chain reaction for the rapid construction of synthetic genes / Patrick J. Dillon, Craig A. Rosen -- Recombinant circle polymerase chain reaction for site-directed mutagenesis / Douglas H. Jones -- Site-directed mutagenesis by double polymerase chain reaction : megaprimer method / Sailen Barik -- Generation of a polymerase chain reaction renewable source of subtractive cDNA / W. Michael Kuehl, James Battey -- PCR-based full-length cDNA cloning utilizing the universal-adaptor/specific DOS primer-pair strategy / David L. Cooper, Narayana R. Isola -- Use of degenerate oligonucleotide primers and the polymerase chain reaction to clone gene family members / Gregory M. Preston -- Single specific primer-polymerase chain reaction (SSP-PCR) and genome walking / Venkatakrishna Shyamala, Giovanna Ferro-Luzzi Ames -- cDNA cloning by inverse polymerase chain reaction / Sheng-He Huang, Chun-Hua Wu, Bing Cai, John Holcenberg -- Amplification of gene ends from gene libraries by polymerase chain reaction with single-sided specificity / Sheng-He Huang, Ambrose Y. Jong, Wu Yang, John Holcenberg -- Anchoring a defined sequence to the 55' ends of mRNAs : the bolt to clone rare full length mRNAs and generate cDNA libraries from a few cells / Jean Baptiste Dumas Milne Edwards, Jacques Delort, Jacques Mallet |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Polymerase chain reaction -- Methodology
|
|
Polymerase Chain Reaction -- methods
|
|
Polymerase chain reaction -- Methodology
|
| Genre/Form |
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
White, Bruce Alan.
|
| ISBN |
9781592595020 |
|
1592595022 |
|
