| Description |
1 online resource : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 2066 |
|
Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; v. 2066. 1064-3745
|
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Springer protocols (Series) 1949-2448
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| Summary |
This volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice. The chapters in this book cover topics such as pronuclear microinjection in one-cell embryos; embryo transfer surgery; nuclear transfer and cloning; blastocyst microinjection; and cryobanking and recovery of genetically modified mice. Importantly, there are chapters devoted to the latest application of CRISPR technology, as well as the establishment of induced pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed September 24, 2019) |
| Subject |
Transgenic mice.
|
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Mice -- Genetic engineering
|
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Mice as laboratory animals.
|
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Mice, Transgenic -- genetics
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Gene Transfer Techniques
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Mice, Transgenic
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Mice as laboratory animals
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Mice -- Genetic engineering
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Transgenic mice
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
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| Author |
Larson, Melissa A., editor
|
| ISBN |
9781493998371 |
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1493998374 |
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