| Description |
1 online resource (xiv, 576 pages) : illustrations |
| Series |
Methods in molecular biology, 1940-6029 ; volume 2102 |
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Methods in molecular biology (Clifton, N.J.) ; v. 2102. 1064-3745
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| Contents |
Determination of Mutational Spectra Induced by Environmental Toxicants in Complex Human Cell Populations Robert Gealy and Phouthone Keohavong 18. Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice Vasily N. Dobrovolsky, Xuefei Cao, Javed A. Bhalli, and Robert H. Heflich 19. Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes Vasily N. Dobrovolsky, Joseph G. Shaddock, and Robert H. Heflich 20. Molecular Analysis of Mutations in the Human HPRT Gene Phouthone Keohavong, Liqiang Xi, and Stephen G. Grant 21. Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions In Human Lymphocytes James C. Fuscoe Part VII: Detection and Characterization of Cancer Gene Mutation 22. Analysis of Mutations in K-ras and p53 Genes in Sputum and Plasma Samples Lifang Zhang, Wei-Min Gao, and Phouthone Keohavong 23. ACB-PCR Quantification of Low-Frequency Hotspot Cancer-Driver Mutations Meagan B. Myers, Karen L |
| Summary |
This fully revised book includes new and improved protocols to analyze toxicity at the DNA, RNA, and protein levels using genomic, epigenomic, transcriptomic, and proteomic approaches. Sections of the book address key subjects such as bioinformatics and biostatistics, toxicant-induced lung inflammation and tumors, analysis of protein and gene expression and silencing, gene promoter methylation, detection of chromosomal and genome-wide damage, analysis of DNA-adducts and surrogate gene mutations, detection and characterization of cancer gene mutation, and many other areas of study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Molecular toxicology.
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Genetics (non-medical)
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Medical genetics.
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Pharmacology.
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Science -- Life Sciences -- Genetics & Genomics.
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Medical -- Genetics.
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Medical -- Pharmacology.
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Molecular toxicology
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Keohavong, Phouthone, editor
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Singh, Kamaleshwar P., editor
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Gao, Weimin, editor
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| ISBN |
9781071602232 |
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1071602233 |
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1071602225 |
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9781071602225 |
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