Title Candida species : methods and protocols / edited by Richard Calderone, Ronald Cihlar

Published New York, NY : Humana Press, 2016

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Description 1 online resource (xi, 240 pages) : illustrations (some color) Series Methods in Molecular Biology, 1064-3745 ; 1356 Springer protocols, 1949-2448 Methods in molecular biology (Clifton, N.J.) ; v. 1356. 1064-3745 Springer protocols (Series) 1949-2448 Contents How to use the Candida Genome Database -- Transcriptional Profiling of Candida albicans in the Host -- SRYTH: A New Yeast Two-Hybrid Method -- Chromatin immunoprecipitation (ChIP) assay in Candida albicans -- Assessing mitochondrial functions in Candida albicans -- Imaging Candida infections in the host -- Identification of Secreted Candida Proteins using Mass Spectrometry -- In vitro models for Candida Biofilm Development -- The Fungal Biome of the Oral Cavity -- Experimental models of C. albicans-streptococcal Co-infection -- Staphylococcus-Candida interaction models: Antibiotic Resistance Testing and Host Interaction -- Genetic Screens for Determination of Mechanism of Action -- Microbroth Dilution Susceptibility Testing of Candida Species -- Biofilms and Antifungal Susceptibility Testing -- Typing Candida Species using Microsatellite Length Polymorphism and Multilocus Sequence Typing -- Diagnostic Methods for Detection of Blood-borne Candidiasis Summary This volume provides detailed discussion of a variety of important techniques that researchers use to study fungal molecular biology and pathogenesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Candida Species: Methods and Protocols aims to ensure successful results in the further study of this vital field Bibliography Includes bibliographical references and index Subject Candida. Life sciences. Bacteriology. Candida albicans. Candidiasis. Pathogenic fungi. Candida albicans -- pathogenicity Candida albicans -- genetics Candidiasis Candida Biological Science Disciplines Bacteriology Candida albicans biological sciences. Candida albicans Pathogenic fungi Candidiasis Candida Bacteriology Life sciences Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Calderone, Richard A., 1942- editor. Cihlar, Ronald L., editor ISBN 9781493930524 1493930524 1493930516 9781493930517

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