| Description |
1 online resource (184 p.) |
| Contents |
Intro -- Biosensors for Single-Cell Analysis -- Copyright -- Contents -- Contributors -- Foreword -- Acknowledgment -- Chapter 1 Introduction to biosensors for single-cell analysis with historical developments highlighted -- 1 Introduction to biosensors for single-cell analysis -- 2 Historical developments of biosensors for single-cell analysis -- 3 Book structure -- Acknowledgment -- References -- Chapter 2 Biosensors for single-cell proteomic characterization -- 1 Introduction -- 2 Antibody-based SCP characterization by microfluidic biosensor -- 2.1 Microchamber-based biosensor -- 2.2 Microwell-based biosensor -- 2.3 Droplet-based biosensor -- 2.4 Microfluidic flow cytometry -- 2.5 Single-cell Western blot -- 3 Substrate-based characterization of SCP by microfluidic biosensor -- 4 Chip-MS for SCP characterization -- 5 Commercial microfluidic-based biosensing platform for SCP characterization -- 6 Conclusion and perspectives -- Acknowledgment -- References -- Chapter 3 Biosensors for single-cell metabolomic characterization -- 1 Introduction -- 2 Fluorescence -- 3 Electrochemical detection -- 4 Mass spectrometry -- 4.1 Vacuum-based single-cell mass spectrometry methods -- 4.1.1 Secondary ion mass spectrometry -- 4.1.2 Matrix-assisted laser desorption ionization -- 4.2 Ambient single-cell mass spectrometry methods -- 4.2.1 Probe-based techniques -- Solid probes -- Monochannel probes -- Multichannel probes -- 4.2.2 Laser-based techniques -- 4.3 Multimodal methods -- 4.3.1 Separation-assisted techniques -- 4.3.2 Microfluidics-based techniques -- 4.3.3 Spectroscopy-assisted techniques -- 5 Conclusion -- Acknowledgment -- References -- Chapter 4 Single-cell electrical characterization -- 1 Introduction -- 2 Basic theory -- 2.1 Cell modeling -- 2.2 Cell suspension and Clausius-Mossotti factor |
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3 Dielectrophoresis (DEP) and electrorotation (ROT) -- 3.1 Dielectrophoresis -- 3.2 Electrorotation -- 3.3 Applications for characterization -- 3.4 Signification of the differences -- 4 Microfluidic impedance cytometry -- 4.1 Discrimination of cell subpopulations -- 4.2 Impedance spectra fitting -- 4.3 Designs of impedance cytometers -- 4.4 Applications -- 5 Recent trends and future developments -- Acknowledgment -- References -- Chapter 5 Biosensors for single-cell mechanical characterization -- 1 Introduction -- 2 Biology background -- 3 Importance of sing-cell mechanical phenotyping -- 4 Conventional methods -- 5 Microfluidics-based methods -- 5.1 Constriction deformability cytometry -- 5.2 Shear flow deformability cytometry -- 5.3 Extensional flow deformability cytometry -- 6 Summary -- References -- Chapter 6 Single-cell analysis by evanescent wave sensing and hyperspectral microscopy -- 1 Introduction -- 2 Label-free optical refractometric biosensors for SCA -- 2.1 Surface plasmon resonance microscopy and imaging methods -- 2.2 Optical waveguides biosensors -- 3 Hyperspectral microscopic imaging methods for SCA -- 3.1 Dark-field hyperspectral imaging -- 3.2 Hyperspectral Raman scattering -- 3.3 Hyperspectral fluorescence microscopy -- 4 Conclusion -- Acknowledgment -- References -- Chapter 7 Future directions of biosensors for single-cell analysis from the perspective of instrument developments -- 1 Introduction -- 2 Instrumentation of hematology analyzer ( Table 1) -- 3 Instrumentation of flow cytometer ( Table 2) -- 4 Future directions -- Acknowledgment -- Index |
| Notes |
Description based upon print version of record |
| Form |
Electronic book
|
| Author |
Lu, Yao
|
| ISBN |
9780323885591 |
|
0323885594 |
|
