| Description |
1 online resource (457 p.) |
| Contents |
Cover -- Half Title -- Title Page -- Copyright Page -- Dedication -- Table of Contents -- Preface -- About the Author -- 1 Introduction: Toolbox at the Physical-Life Science Interface -- 1.1 Motivation for Biophysics -- 1.2 What Do We Mean By a "Toolbox?" -- 1.3 Makeup of the Subsequent Chapters in This Book -- 1.3.1 Detection, Sensing, and Imaging Techniques -- 1.3.2 Experimental Biophysical Methods Primarily Relating Especially to Force -- 1.3.3 Complementary Experimental Technologies -- 1.3.4 Theoretical Biophysics Tools -- 1.4 Once More, Unto the Breach -- 1.5 Summary Points -- Questions |
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2.3.1 Importance of Carbon -- 2.3.2 Lipids and Fatty Acids -- 2.3.3 Amino Acids, Peptides, and Proteins -- 2.3.4 Sugars -- 2.3.5 Nucleic Acids -- 2.3.6 Water and Ions -- 2.3.7 Small Organic Molecules of Miscellaneous Function -- 2.4 Cell Processes -- 2.4.1 Central Dogma of Molecular Biology -- 2.4.2 Detection of Signals -- 2.4.3 Trapping "Negative" Entropy -- 2.4.4 Natural Selection, Neo-Darwinism, and Evolution -- 2.4.5 "Omics" Revolution -- 2.5 Physical Quantities in Biology -- 2.5.1 Force -- 2.5.2 Length, Area, and Volume -- 2.5.3 Energy and Temperature -- 2.5.4 Time |
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2.5.5 Concentration and Mass -- 2.5.6 Mobility -- 2.6 Summary Points -- Questions -- References -- 3 Making Light Work in Biology: Basic, Foundational Detection and Imaging Techniques Involving Ultraviolet, Visible, and Infrared... -- 3.1 Introduction -- 3.2 Basic UV-VIS-IR Absorption, Emission, and Elastic Light Scattering Methods -- 3.2.1 Spectrophotometry -- 3.2.2 Fluorimetry -- 3.2.3 Flow Cytometry and Fluorescence-Assisted Cell Sorting -- 3.2.4 Polarization Spectroscopy -- 3.2.5 Optical Interferometry -- 3.2.6 Photothermal Spectroscopy -- 3.3 Light Microscopy: The Basics |
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3.3.1 Magnification -- 3.3.2 Depth of Field -- 3.3.3 Light Capture From the Sample -- 3.3.4 Photon Detection at the Image Plane -- 3.4 Nonfluorescence Microscopy -- 3.4.1 Bright-Field and Dark-Field Microscopy -- 3.4.2 Contrast Enhancement Using Optical Interference -- 3.4.3 Digital Holographic Microscopy -- 3.5 Fluorescence Microscopy: The Basics -- 3.5.1 Excitation Sources -- 3.5.2 Fluorescence Emission -- 3.5.3 Multicolor Fluorescence Microscopy -- 3.5.4 Photobleaching of Fluorophores -- 3.5.5 Organic Dye Fluorophores -- 3.5.6 FlAsH/ReAsH Probes |
| Summary |
Biophysics: Tools and Techniques for the Physics of Life covers the experimental, theoretical and computational tools and techniques of biophysics. It addresses the purpose, science, and application of all physical science instrumentation, theoretical analysis and biophysical computational methods used in current research labs |
| Notes |
Description based upon print version of record |
| Bibliography |
References -- 2 Orientation for the Bio-Curious: The Basics of Biology for the Physical Scientist -- 2.1 Introduction: The Material Stuff of Life -- 2.2 Architecture of Organisms, Tissues, and Cells and the Bits Between -- 2.2.1 Cells and Their Extracellular Surroundings -- 2.2.2 Cells Should Be Treated Only as a "Test Tube of Life" With Caution -- 2.2.3 Cells Categorized By the Presence of Nuclei (Or Not) -- 2.2.4 Cellular Structures -- 2.2.5 Cell Membranes and Walls -- 2.2.6 Liquid-Liquid Phase-Separated (LLPS) Biomolecular Condensates -- 2.2.7 Viruses -- 2.3 Chemicals That Make Cells Work |
| Notes |
3.5.7 Semiconductors, Metal-Based Fluorophores, and Nanodiamonds |
| Form |
Electronic book
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| ISBN |
9781003806523 |
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100380652X |
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