Preface -- ch. 1. Overview of bioluminescence; examples of bioluminescent reactions -- ch. 2. The family of fluorescent proteins -- ch. 3. Bioluminescence- and fluorescence-based cell viability and proliferation assays -- ch. 4. Real-time in vivo monitoring of gene expression by bioluminescence and fluorescence imaging -- ch. 5. Bioluminescence and fluorescence imaging for in vivo real-time monitoring of key metabolites and the intracellular environment -- ch. 6. Bioluminescence and fluorescence imaging for in vivo monitoring of protein-protein interactions -- ch. 7. Fluorescence and bioluminescence tomography: current status and perspectives -- ch. 8. Review of available instrumentation for in vivo bioluminescence and fluorescence imaging
Summary
This book is intended for scientists and students involved in basic cell physiology research, for industry professionals, engineers, and managers involved in drug discovery and pre-clinical drug development. It will discuss the practical aspects of luminescence in vivo imaging for monitoring of intracellular processes. While some basic knowledge of biochemistry and biophysics is preferable, the book will include a brief review of fundamental principles that will allow people not familiar with these disciplines to grasp basic concepts
