Description |
1 online resource (xi, 356 pages) : illustrations (some color) |
Series |
Methods in Molecular Biology ; 1320 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1320. 1064-3745
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Contents |
Perspectives and pitfalls in nucleic acids crystallography / Eric Westhof -- Preparation of short 5'-triphosphorylated oligoribonucleotides for crystallographic and biochemical studies/ Nikita Vasilyev and Alexander Serganov -- Preparation and crystallization of riboswitches / Alla Peselis, Ang Gao, and Alexander Serganov -- In vitro/in vivo production of tRNA for x-ray studies / Clément Dégut, Alexandre Monod, Franck Brachet, Thibaut Crépin, and Carine Tisné -- Polyacrylamide gel electrophoresis for purification of large amounts of RNA / Mélanie Meyer and Benoît Masquida -- Use of the U1A protein to facilitate crystallization and structure determination of large RNAs / Adrian R. Ferré-D'Amaré -- Fab chaperone-assisted RNA crystallography (Fab CARC) / Eileen Sherman, Jennifer Archer, and Jing-Dong Ye -- Generating crystallographic models of DNA dodecamers from structures of RNase H : DNA complexes / Martin Egli and Pradeep S. Pallan -- Isothermal titration calorimetry : assisted crystallization of RNA-ligand complexes / Cyrielle da Veiga, Joelle Mezher, Philippe Dumas, and Eric Ennifar -- Crystallographic data and model quality / Kay Diederichs -- Advanced crystallographic data collection protocols for experimental phasing / Aaron D. Finke, Ezequiel Panepucci, Clemens Vonrhein, Meitian Wang, Gérard Bricogne, and Vincent Oliéric -- Nucleic acid crystallography via direct selenium derivatization : RNAs modified with se-nucleobases / Huiyan Sun, Sibo Jiang, and Zhen Huang -- Practical radiation damage-induced phasing / Chloe Zubieta and Max H. Nanao -- Soaking hexammine cations into RNA crystals to obtain derivatives for phasing diffraction data / Robert T. Batey and Jeffrey S. Kieft -- Using molecular replacement phasing to study the structure and function of RNA / Marco Marcia -- Helical symmetry of nucleic acids : obstacle or help in structure solution? / Alexandre Urzhumtsev, Ludmila Urzhumtseva, and Ulrich Baumann -- RNA structure refinement using the ERRASER-phenix pipeline / Fang-Chieh Chou, Nathaniel Echols, Thomas C. Terwilliger, and Rhiju Das -- Neutron nucleic acid crystallography / Toshiyuki Chatake -- Reconstitution of functionally active thermus thermophilus 30S ribosomal subunit from ribosomal 16S RNA and ribosomal proteins / Sultan Agalarov, Marat Yusupov, and Gulnara Yusupova -- Crystallographic studies of the ribosomal A-site molecular wwitches by using model RNA oligomers / Jiro Kondo -- Structure of the HCV internal ribosome entry site subdomain IIa RNA in complex with a viral translation inhibitor / Sergey M. Dibrov and Thomas Hermann -- Anions in nucleic acid crystallography / Luigi D'Ascenzo and Pascal Auffinger |
Summary |
"This volume includes comprehensive and up-to-date coverage of all nucleic-acid-specific steps used in X-ray crystallography, from macromolecule production to structure determination. Chapters dedicated to RNA preparation and crystallogenesis will be of interest to beginners, while chapters focused on data collection, phasing and refinement will be particularly useful to researchers with a higher level of expertise. Several functional case studies are also presented in the last part of the book. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Nucleic Acid Crystallography: Methods and Protocols presents protocols that are aimed at both researchers and students who are interested in the structural biology of DNA or RNA, alone or in complex with proteins or ligands"-- Back cover |
Analysis |
levenswetenschappen |
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life sciences |
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nucleic acids |
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chemie |
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chemistry |
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Biology (General) |
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Biologie (algemeen) |
Bibliography |
Includes bibliographical references and index |
Subject |
Nucleic acids -- Laboratory manuals
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Crystallography -- Laboratory manuals
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Nucleic acids.
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Crystallography.
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Nucleic Acids
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Crystallography
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Biochemistry.
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Science -- Life Sciences -- Biochemistry.
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Crystallography
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Nucleic acids
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Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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Form |
Electronic book
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Author |
Ennifar, Eric, editor.
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ISBN |
9781493927630 |
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1493927639 |
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1493927620 |
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9781493927623 |
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