Title Extracellular matrix protocols

Edition 2nd ed. / edited by Sharona Even-Ram and Vira V. Artym Published New York : Humana, ©2009

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Description 1 online resource (xi, 375 pages) : illustrations Series Methods in molecular biology, 1064-3745 ; 522 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 522 Springer protocols (Series) Contents Retroviral delivery of ECM genes / Vitali alexeev and Olga Igoucheva -- Tissue-specific KO of ECM proteins / Mara Brancaccio, Emila Turco, and Emilio Hirsch -- Recombinant collagen trimers from insect cells and yeast / Johanna Myllyharju -- Eukaryotic expression and purification of recombinant extracellular matrix proteins carrying the Strep II tag / Neil Smyth [and others] -- Preparation of recombinant fibronectin fragments for functional and structural studies / David Staunton [and others] -- Quantitative determination of collagen cross-links / Nicholas C. Avery, Trevor J. Sims, and Allen J. Bailey -- ECM macromolecules : height-mapping and nano-mechanics using atomic force microscopy / Nigel W. Hodson, Cay M. Kielty, and Michael J. Sherratt -- Atomic force microscopy measurements of intermolecular binding forces / Gradimir N. Misevic, Yannis Karamanos, and Nikola J. Misevic -- Mass-mapping of ECM macromolecules by scanning transmission electron microscopy / Michael J. Sherratt [and others] -- Chemical microscopy of biological samples by dynamic mode secondary ion mass spectrometry / Gradimir N. Misevic [and others] -- ECM macromolecules : rotary shadowing and transmission electron microscopy / Michael J. Sherratt [and others] -- Using self-assembled monolayers to pattern ECM proteins and cells on substrates / Emanuele Ostuni [and others] -- Solid phase assays for studying ECM protein-protein interactions / A. Paul Mould -- Cell adhesion assays / Martin J. Humphries -- ECM degradation assays for analyzing local cell invasion / Vira V. Artym, Kenneth M. Yamada, and Susette C. Mueller -- Fluorescence-based assays for in vitro analysis of cell adhesion and migration / Paola Spessotto [and others] -- Fibrin gel model for assessment of cellular contractility / Sharona Even-Ram -- Fluorescent labeling techniques for investigation of fibronectin fibrillogenesis (labeling fibronectin fibrillogenesis) / Roumen Pankov and Albena Momchilova -- Stromagenesis during tumorigenesis : characterization of tumor-associated fibroblasts and stroma-derived 3D matrices / Remedios Castelló-Cros and Edna Cukierman -- Tissue recombinants to study extracellular matrix targeting to basement membranes / Patricia Simon-Assmann [and others] -- ECM and FGF-dependent assay of embryonic SMG epithelial morphogenesis : investigating growth factor/matrix regulation of gene expression during submandibular gland development / Ivan T. Rebustini and Matthew P. Hoffman -- Analyzing how cell adhesion controls mammary gland function by transplantation of embryonic mammary tissue from knockout mice / Teresa C.M. Klinowska and Charles H. Streuli -- Characterizing ECM production by cells encapsulated in hydrogels / Iossif A. Strehin and Jennifer H. Elisseeff -- Tissue engineering and cell-populated collagen matrices / Paul D. Kemp Summary In this edition a variety of methods is compiled that can be readily reproduced in most cell biology laboratories, as well as several cutting-edge technologies that are available in a limited number of centers, but are well worth the awareness and exposure to the extracellular matrix research community. The book chapters are divided into sections that represent molecular biology techniques to study gene expression, biophysical and biochemical methods for the analysis of structure and composition, cell biology methods for the assessment of cell behavior and matrix assembly and tissue engineering applications Analysis dieren animals cellen cells celstructuur cell structure cytologie cytology dierlijke weefsels animal tissues biologische technieken biological techniques protocollen protocols Cellular Biology Celbiologie Notes Previous edition: edited by Charles H. Streuli and Michael E. Grant. Totowa, N.J.: Humana, 2000 Bibliography Includes bibliographical references and index Notes Print version record Subject Extracellular matrix -- Laboratory manuals Extracellular matrix proteins -- Laboratory manuals Extracellular matrix. Extracellular Matrix Extracellular Matrix Proteins Extracellular Matrix. Extracellular Matrix Proteins. Extracellular matrix Extracellular matrix proteins Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Even-Ram, Sharona Artym, Vira V Streuli, Charles H. Extracellular matrix protocols LC no. 2008942787 ISBN 9781597454131 1597454133 9781607612681 1607612682 9781617378676 1617378674

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