| Description |
1 online resource (xvii, 548 pages) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; v. 268 |
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Methods in molecular biology (Clifton, N.J.) ; v. 268.
|
| Contents |
Detection of tetracycline resistance genes by PCR methods / R.I. Aminov [and others] -- Integron analysis and genetic mapping of antimicrobial resistance genes in salmonella enterica serotype typhimurium / M. Daly and S. Fanning -- Molecular-based identification and typing of campylobacter jejuni and C. coli / B. Lucey, F. O'Halloran and S. Fanning -- Molecular genotyping methods and computerized analysis for the study of salmonella enterica / A.B. Vivanco [and others] -- Detection of infectious rotaviruses by flow cytometry / A. Bosch [and others] -- Integrated cell culture/PCR for detection of enteric viruses in environmental samples / K.A. Reynolds -- Abundance in sewage of bacteriophages infecting escherichia coli o157:H7 / M. Muniesa and J. Jofre -- Molecular genotyping of Irish rotavirus strains / F. O'Halloran and S. Fanning -- Hepatitis a virus: Molecular detection and typing / G. Sanchez [and others] -- Typing fungal isolates: Molecular methods and computerized analysis / A. Rementeria [and others] -- Fungal isolation and enumeration in foods / D.J. Bueno, J.O. Silva and G. Oliver -- Determination of aflatoxins and zearalenone in different culture media / D.J. Bueno and G. Oliver -- Intracellular multiplication of legionella species and the influence of amoebae on their intracellular growth in human monocytes: Mono Mac 6 cells and acanthamoeba castellanii as suitable in vitro models / B. Neumeister -- Viability of amoebae, fungal conidia, and yeasts: Rapid assessment by flow cytometry / J.A. Noble-Wang [and others] -- Detection and differentiation of cryptosporidium oocysts in water by PCR-RFLP / L. Xiao, A.A. Lal and J. Jiang -- Genotyping of cryptosporidium parvum with microsatellite markers / G. Widmer, X. Feng and S. Tanriverdi -- Immunomagnetic separation of pathogenic organisms from environmental matrices / G.P. Yakub and K.L. Stadterman-Knauer -- Detection of erysipelothrix rhusiopathiae in clinical and environmental samples / S.G. Fidalgo and T.V. Riley -- Interaction between lactic acid bacteria and gastrointestinal nematodes of caprine origin / D. Draksler, M.C. Monferran and S. Gonzalez -- Molecular detection of genes responsible for cyanobacterial toxin production in the genera microcystis, nodularia, and cylindrospermopsis / B.P. Burns [and others] -- Purification of antilisterial bacteriocins / J.M. Berjeaud and Y. Cenatiempo -- The hazard analysis and critical control point system in food safety / A.G. Herrera -- Testing disinfectants in the food factory: Phenol coefficient method / A.G. Herrera -- Nontraditional method of evaluating disinfectants: With isolated microorganisms from the food factory / A.G. Herrera -- Microbiological analysis of cosmetics / A.G. Herrera -- Helicobacter pylori and food products: A public health problem / A.G. Herrera -- Microbiological test for sanitation of equipment in the food factory / A.G. Herrera -- Spectrophotometric determination of histamine in fisheries using an enzyme immunoassay method / T.L. Pessatti, J.D. Fontana and M.L. Pessatti -- Flavonoids from Argentine tagetes (asteraceae) with antimicrobial activity / M.L. Tereschuk [and others] -- Purification of bacteriocins produced by lactic acid bacteria / L. Saavedra, P. Castellano and F. Sesma -- Production of antimicrobial substances by lactic acid bacteria i: Determination of hydrogen peroxide / M.S. Tomas [and others] -- Production of antimicrobial substances by lactic acid bacteria ii: Screening bacteriocin-producing strains with probiotic purposes and characterization of a lactobacillus bacteriocin / V.S. Ocana and M. Elena Nader-Macias -- Statistical models to optimize production of probiotic characteristics / M.S. Tomas, E. Bru and M. Elena Nader-Macias -- Meat-model system development for antibacterial activity determination / G. Vignolo and P. Castellano -- Colonization capability of lactobacilli and pathogens in the respiratory tract of mice: Microbiological, cytological, structural, and ultrastructural studies / R. Cangemi de Gutierrez, V.M. Santos and M.E. Nader-Macias -- Effects of estrogen administration on the colonization capability of lactobacilli and escherichia coli in the urinary tracts of mice / C. Silva, R. Rey and M. Elena Nader-Macias -- Effect of lactobacilli administration in the vaginal tract of mice: Evaluation of side effects and local immune response by local administration of selected strains / E. Vintini, V. Ocana and M. Elena Nader-Macias -- Determination of bacterial adhesion to intestinal mucus / C. Gusils, V. Morata and S. Gonzalez -- Animal model for in vivo evaluation of cholesterol reduction by lactic acid bacteria / M.P. Taranto [and others] -- Assessing survival of dairy propionibacteria in gastrointestinal conditions and adherence to intestinal epithelia / G. Zarate, S. Gonzalez and A.P. Chaia -- Bacterial surface characteristics applied to selection of probiotic microorganisms / M.C. Otero, V.S. Ocana and M. Elena Nader-Macias -- Adhesion ability of lactobacillus to vaginal epithelial cells: Study by microbiological methods / V.S. Ocana and M. Elena Nader-Macias -- Hydroxylapatite beads as an experimental model to study the adhesion of lactic acid bacteria from the oral cavity to hard tissues / C. Ostengo Mdel and M. Elena Nader-Macias -- Microtechnique for identification of lactic acid bacteria / C. Gusils [and others] -- Differentiation of lactic acid bacteria strains by postelectrophoretic detection of esterases / R.B. Medina, M.B. Katz and S. Gonzalez -- Determination of esterolytic and lipolytic activities of lactic acid bacteria / R.B. Medina [and others] -- Meat-model system development for proteolytic activity determination / G. Vignolo [and others] -- Beta-glucuronidase method to determine mastitis levels in goat milk / R. Oliszewski [and others] -- Differences between biogenic amine detection by HPLC methods using opa and dansyl derivates / M.R. Alberto, M.E. Arena and M.C. De Nadra -- Microflora of the gastrointestinal tract: A review / W.L. Hao and Y.K. Lee -- Public health implications related to spread of pathogens in manure from livestock and poultry operations / J.L. Spencer and J. Guan -- Molecular aspects of disease pathogenesis in the transmissible spongiform encephalopathies / S.A. Priola and I. Vorberg |
| Summary |
The world abounds with microorganisms that are hazardous to human health in the normal course of life. In Public Health Microbiology: Methods and Protocols, expert investigators describe in step-by-step detail the laboratory techniques they have perfected for determining the nature and character of a diverse group of hazardous bacteria, viruses, and fungi. For bacterial and viral pathogens, the authors give special attention to PCR methods for detecting genes resistant to tetracycline, for resistance in Salmonella enterica, for identifying and typing Campylobacter coli, and for detecting the abundance of enteric, hepatitis A, and rotaviruses in sewage, as well as bacteriophages infecting the O157:H7 strain of Escherichia coli. For fungi the authors offer methods for computerized analysis and typing of fungal isolates, for the isolation and enumeration of fungi in foods, and for the determination of aflatoxin and zearalenone. Other methods deal with hazard analysis, the use of disinfectants, microbiological analysis of cosmetics, microbiological tests for sanitation equipment in factories, and the uses of animals in studies of various characteristics of lactobacilli. Review articles address the spread of pathogens from livestock and poultry production, including the threat of prion-based diseases. All protocols presented follow the successful Methods in Molecular Biology"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Timely and highly practical, Public Health Microbiology: Methods and Protocols offers researchers and public health specialists alike a wide-ranging collection of methods for the determination of many important pathogenic microorganisms, their effects on health, and possible measures that can be taken to counter these effects |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| Subject |
Diagnostic microbiology.
|
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Microbial ecology.
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Environmental Microbiology
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Microbiological Techniques
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MEDICAL -- Microbiology.
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Microbial ecology
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Diagnostic microbiology
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Spencer, J. F. T
|
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Ragout de Spencer, Alicia L
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| ISBN |
9781592597666 |
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1592597661 |
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1588291170 |
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9781588291172 |
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128036033X |
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9781280360336 |
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9786610360338 |
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6610360332 |
|
