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Book Cover
E-book

Title Diagnostic bacteriology protocols / edited by Louise O'Connor
Edition 2nd ed
Published Totowa, N.J. : Humana Press, ©2006

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Description 1 online resource (x, 225 pages) : illustrations
Series Methods in molecular biology, 1064-3745 ; 345
Methods in molecular biology (Clifton, N.J.) ; v. 345
Contents A review of current and future molecular diagnostic tests for use in the microbiology laboratory / Geert Jannes and Daniel De Vos -- Overview of DNA purification for nucleic acid-based diagnostics from environmental and clinical samples / Knut Rudi and Kjetill S. Jakobsen -- Microarray-based detection of bacteria by on-chip PCR / Georg Mitterer and Wolfgang M. Schmidt -- An array biosensor for detection of bacterial and toxic contaminants of foods / Miriam M. Ngundi and Chris R. Taitt -- Detection of Neisseria meningitidis, streptococcus pneumoniae, and Haemophilus influenzae in blood and cerebrospinal fluid using fluorescence-based PCR / Stuart C. Clarke -- Use of hybridization probes in a real-time PCR assay on the LightCycler for the detection of methicillin-resistant Staphylococcus aureus / Andrea J. Grisold and Harald H. Kessler -- Detection of verotoxin genes VT 1 and VT 2 in Escherichia coli O157:H7 in minced beef using immunocapture and real-time PCR / Justine Fitzmaurice -- Application of two-step quantitative reverse-transcription PCR to bacterial diagnostics / Barry Glynn
Summary Carrying on the high standards of the much respected first edition, Diagnostic Bacteriology Protocols, Second Edition, has been fully updated to include the novel identification, detection, and typing technologies that are transforming the field of diagnostic testing. The authors, working at the forefront of diagnostic test development, highlight these new technologies and describe current and future molecular diagnostic tests and related nucleic acid extraction methods. The protocols range from advanced molecular detection, quantification, and typing systems, to protocols for diagnostic protein identification, serological testing, and cell culture-based assays. The emphasis is on nucleic acid-based diagnostics and alternative biochemically and immunologically based formats that can provide significant potential for multiparameter testing and automation. Several protocols describe specific organisms that may be easily modified to detect other species of interest. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Forward looking and highly practical, Diagnostic Bacteriology Protocols, Second Edition illuminates the cutting-edge technologies now available for bacterial pathogen detection and identification and demonstrates their power and ease of use in a wide variety of applications
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Diagnostic bacteriology -- Laboratory manuals
Bacteriology -- Technique.
Bacteriological Techniques
Laboratory Techniques and Procedures
MEDICAL -- Infectious Diseases.
Diagnostic bacteriology
Genre/Form Handbooks and manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author O'Connor, Louise, Dr.
ISBN 158829594X
1597451436
9781597451437
9781588295941
1280832339
9781280832338
9786610832330
6610832331
9781617376665
1617376663