Title Viral vectors for gene therapy : methods and protocols / edited by Curtis A. Machida

Published Totowa, N.J. : Humana Press, ©2003

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Description 1 online resource (xv, 589 pages) : illustrations Series Methods in molecular medicine ; 76 Methods in molecular medicine ; 76. 1543-1894 Contents Use of the herpes simplex viral genome to construct gene therapy vectors / E.A. Burton [and others] -- Construction of multiply disabled herpes simplex viral vectors for gene delivery to the nervous system / C.E. Lilley and R.S. Coffin -- Improved HSV-1 amplicon packaging system using ICP27-deleted, oversized HSV-1 BAC DNA / Y. Saeki, X.O. Breakefield and E.A. Chiocca -- Herpes simplex amplicon vectors / C.J. Link, N.N. Vahanian and S. Wang -- Strategies to adapt adenoviral vectors for targeted delivery / C.R. O'Riordan, A. Song and J. Lanciotti -- Use of recombinant adenovirus for gene transfer into the rat brain. Evaluation of gene transfer efficiency, toxicity, and inflammatory and immune reactions / A. Hurtado-Lorenzo [and others] -- Generation of adenovirus vectors devoid of all viral genes by recombination between inverted repeats / H. Stecher [and others] -- Packaging cell lines for generating replication-defective and gutted adenoviral vectors / J.S. Chamberlain, C. Barjot and J. Scott -- Improving the transcriptional regulation of genes delivered by adenovirus vectors / S. Rubinchik [and others] -- Targeted integration by adeno-associated virus / M.D. Weitzman [and others] -- Development and optimization of adeno-associated virus vector transfer into the central nervous system / M.J. During [and others] -- A method for helper virus-free production of adeno-associated virus vectors / R.F. Collaco and J.P. Trempe -- Novel tools for production and purification of recombinant adeno-associated viral vectors / J.D. Harris, S.G. Beattie and J.G. Dickson -- Recombinant adeno-associated viral vector types 4 and 5. Preparation and application for CNS gene transfer / B.L. Davidson and J.A. Chiorini -- Trans-splicing vectors expand the packaging limits of adeno-associated virus for gene therapy applications / D. Duan [and others] -- Generation of retroviral packaging and producer cell lines for large-scale vector production with improved safety and titer / T.W. Dubensky, Jr. and S.L. Sauter -- An ecdysone-inducible expression system for use with retroviruses / K. Morse and J. Olsen -- In vivo infection of mice by replication-competent MLV-based retroviral vectors / E. Bachrach [and others] -- Development of simian retroviral vectors for gene delivery / B. Li and C.A. Machida -- Self-inactivating lentiviral vectors and a sensitive Cre-loxP reporter system / L.J. Chang and A.K. Zaiss -- Lentiviral vectors for gene transfer to the central nervous system. Applications in lysosomal storage disease animal models / D.J. Watson and J.H. Wolfe -- A highly efficient gene delivery system derived from feline immunodeficiency virus (FIV) / S.L. Sauter, M. Gasmi and T.W. Dubensky, Jr. -- A multigene lentiviral vector system based on differential splicing / Y. Zhu and V. Planelles -- Production of trans-lentiviral vector with predictable safety / J.C. Kappes, X. Wu and J.K. Wakefield -- Human immunodeficiency virus type 1-based vectors for gene delivery to human hematopoietic stem cells / A. Ramezani and R.G. Hawley -- Semliki Forest virus vectors for gene transfer / J. Wahlfors and R.A. Morgan -- Semliki Forest virus (SFV) vectors in neurobiology and gene therapy / K. Lundstrom and M.U. Ehrengruber -- Semliki Forest virus vectors for large-scale production of recombinant proteins / K. Lundstrom -- Development of foamy virus vectors / G. Vassilopoulos, N.C. Josephson and G. Trobridge -- Poxviral/retroviral chimeric vectors allow cytoplasmic production of transducing defective retroviral particles / G.W. Holzer and F.G. Falkner Summary The promise of gene therapy can be realized only if workable vectors can be found to deliver therapeutic genes. In Viral Vectors for Gene Therapy: Methods and Protocols, leading researchers from academia and biotechnology describe proven molecular methods for the construction, development, and use of virus vectors for gene transfer and gene therapy. Offering detailed step-by-step instructions to ensure successful results, these experts detail the use of herpes viruses, adenoviruses, adeno-associated viruses, simple and complex retroviruses, including lentiviruses, and other virus systems for vector development and gene transfer. Additional chapters demonstrate the use of virus vectors in the brain and central nervous system. Each protocol includes a discussion of the principles involved, numerous charts and tables, ample references, and notes on possible problems, troubleshooting, and alternative procedures. Comprehensive and highly practical, Viral Vectors for Gene Therapy: Methods and Protocols provides not only researchers with the basic tools needed to design targeted gene delivery vectors, but also clinicians with an understanding of how to apply viral vectors to the treatment of genetic disorders Bibliography Includes bibliographical references and index Notes English Print record version In Springer Protocols Subject Gene therapy -- Laboratory manuals Genetic vectors -- Laboratory manuals Transfection -- Laboratory manuals Viral genetics -- Laboratory manuals Genetic vectors. Gene therapy. Viruses. Genetic Vectors Genetic Therapy Gene Transfer Techniques Viruses HEALTH & FITNESS -- Diseases -- Genetic. MEDICAL -- Genetics. Gene therapy Genetic vectors Transfection Viral genetics Genre/Form Handbooks and manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Machida, Curtis A ISBN 9781592593040 1592593046 1280841907 9781280841903 9786610841905 661084190X

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