Title Phytoplasma : methods and protocols / edited by Matt Dickinson, Jennifer Hodgetts

Published New York : Humana Press, ©2013

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Description 1 online resource (xii, 421 pages) : illustrations (some color) Series Methods in molecular biology, 1940-6029 ; 938 Methods in molecular biology (Clifton, N.J.) ; 938. 1064-3745 Contents The phytoplasmas: an introduction / Matt Dickinson, Melanie Tuffen, and Jennifer Hodgetts -- Techniques for the maintenance and propagation of phytoplasmas in glasshouse collections of Catharanthus roseus / Jennifer Hodgetts, David Crossley, and Matt Dickinson -- Micropropagation and maintenance of phytoplasmas in tissue culture / Assunta Bertaccini, Samanta Paltrinieri, Marta Martini, Mara Tedeschi, and Nicoletta Contaldo -- Dodder transmission of phytoplasmas / Jaroslava Přibylová and Josef Špak -- Insect maintenance and transmission / Heather Kingdom -- Capturing insect vectors of phytoplasmas / Phyllis Weintraub and Jürgen Gross -- Insect vector transmission assays / Domenico Bosco and Rosemarie Tedeschi -- Molecular identification of phytoplasma vector species / Sabrina Bertin and Domenico Bosco -- Dienes' staining and light microscopy for phytoplasma visualization / Rita Musetti -- DAPI staining and fluorescence microscopy techniques for phytoplasmas / Nancy M. Andrade and Nolberto L. Arismendi -- Visualization of phytoplasmas using electron microscopy / B. Jean Devonshire -- Automated DNA extraction for large numbers of plant samples / Nataša Mehle, Petra Nikolić, Matevž Rupar, Jana Boben, Maja Ravnikar, and Marina Dermastia -- DNA extraction from arborescent monocots and how to deal with other challenging hosts / Nigel A. Harrison, Robert E. Davis, and Ericka E. Helmick -- Nested PCR and RFLP analysis based on the 16S rRNA gene / Bojan Duduk, Samanta Paltrinieri, Ing-Ming Lee, and Assunta Bertaccini -- PCR and RFLP analyses based on the ribosomal protein operon / Marta Martini and Ing-Ming Lee -- Tuf and secY PCR amplification and genotyping of phytoplasmas / Xavier Foissac, Jean-Luc Danet, Sylvie Malembic-Maher, Pascal Salar, Dana Šafářová, Pavla Válová, and Milan Navrátil -- PCR analysis of phytoplasmas based on the secA gene / Matt Dickinson and Jennifer Hodgetts -- Single-strand conformation polymorphism analysis for differentiating phytoplasma strains / Martina Šeruga Musić and Dijana Škorić -- Microarrays for universal detection and identification of phytoplasmas / Mogens Nicolaisen, Henriette Nyskjold, and Assunta Bertaccini -- T-RFLP for detection and identification of phytoplasmas in plants / Jennifer Hodgetts and Matt Dickinson -- Real-time PCR for universal phytoplasma detection and quantification / Nynne Meyn Christensen, Henriette Nyskjold, and Mogens Nicolaisen -- A real-time PCR detection system for the bois noir and flavescence dorée phytoplasmas and quantification of the target DNA / Nataša Mehle, Nina Prezelj, Matjaž Hren, Jana Boben, Kristina Gruden, Maja Ravnikar, and Marina Dermastia -- Real-time PCR for specific detection of three phytoplasmas from the apple proliferation group / Nataša Mehle, Petra Nikolić, Kristina Gruden, Maja Ravnikar, and Marina Dermastia -- Reverse transcription-PCR for phytoplasma detection utilizing crude sap extractions / Paolo Margaria and Sabrina Palmano -- In-field diagnostics using loop-mediated isothermal amplification / Jenny Tomlinson -- DNA bar-coding for phytoplasma identification / Olga Makarova, Nicoletta Contaldo, Samanta Paltrinieri, Assunta Bertaccini, Henriette Nyskjold, and Mogens Nicolaisen -- Phylogenetic analyses of phytoplasmas based on whole-genome comparison / Hiromi Nishida -- The i PhyClassifier, an interactive online tool for phytoplasma classification and taxonomic assignment / Yan Zhao, Wei Wei, Ing-Ming Lee, Jonathan Shao, Xiaobing Suo, and Robert E. Davis -- Phytoplasma proteomic analysis / Xianling Ji and Yingping Gai -- Preparation of phytoplasma membrane recombinant proteins / Luciana Galetto, Majid Siampour, and Cristina Marzachì -- Phytoplasma plasmid DNA extraction / Mark T. Andersen and Lia W. Liefting -- Cesium chloride-bisbenzimide gradients for separation of phytoplasma and plant DNA / Lucy T.T. Tran-Nguyen and Bernd Schneider -- Pulsed-field gel electrophoresis for isolation of full-length phytoplasma chromosomes from plants / Carmine Marcone -- Mapping the phytoplasma chromosome / Sylvie Malembic-Maher and Patricia Carle Summary Phytoplasmas are a group of bacteria that are capable of multiplying and causing severe diseases in a wide range of plant species and are transported between plants by insect vectors in which the bacteria can also multiply. Phytoplasmas can cause serious and devastating problems for crop plants, especially in developing countries. In Phytoplasmas: Methods and Protocols experts in the field cover topics pertinent to scientists intending to develop a research program on phytoplasmas. The volume opens with an introductory chapter on the importance of phytoplasma diseases, followed by methods for detection and diagnosis as well as techniques for separating and classifying the phytoplasmas into their different taxonomic groups and subgroups. The final chapters cover methods for separating phytoplasma genomic and plasmid DNA from plant DNA for whole genome sequencing. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Phytoplasmas: Methods and Protocols serves as an ideal guide to plant pathologists and molecular biologists aiming to set up a diagnostics facility for identifying the presence of these pathogens in plants or their insect vectors Analysis Life sciences Botany Plant breeding Plant Sciences Plant Genetics & Genomics phytoplasma plant pathogenic bacteria genomica genomics plantengenetica plant genetics plantkunde vectoren, ziekten disease vectors taxonomie taxonomy laboratoriummethoden laboratory methods protocollen protocols biologische technieken biological techniques Agricultural Bacteriology (General) Landbouwkundige bacteriologie (algemeen) Bibliography Includes bibliographical references and index Notes English Online resource; title from PDF title page (Springer Protocols, viewed Oct. 31, 2012) Subject Phytoplasmas -- Laboratory manuals Phytoplasmas. Phytoplasma Phytoplasmas Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Dickinson, Matthew, 1960- Hodgetts, Jennifer ISBN 9781627030892 1627030891 1627030883 9781627030885

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