| Description |
1 online resource (xvi, 631 pages) : illustrations |
| Series |
Methods in molecular medicine ; 70 |
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Methods in molecular medicine ; 70.
|
| Contents |
CFTR mutation detection by multiplex heteroduplex (mhet) analysis on mde gel / J. Zielenski [and others]-Cdna microarrays for pharmacogenomic analysis of cystic fibrosis / M. Srivastava, O. Eidelman and H.B. Pollard-Natural animal models of human genetic diseases / J.J. Wine, M. Dean and D. Glavac-Electrophysiological approach to studying CFTR / H. Fischer-Quantitative analysis of atp-dependent gating of CFTR / A. Powe [and others]-CFTR regulation by phosphorylation / T. Zhu [and others]-Transepithelial measurements of bicarbonate secretion in calu-3 cells / R.J. Bridges-Transepithelial impedance analysis of chloride secretion / A.K. Singh [and others]-Studies of the molecular basis for cystic fibrosis using purified reconstituted CFTR protein / I. Kogan [and others]-Probing CFTR channel structure and function using the substituted-cysteine-accessibility method / M.H. Akabas-Methods for the study of intermolecular and intramolecular interactions regulating CFTR function / A.P. Naren-Fluorescent indicator methods to assay functional CFTR expression in cells / A.S. Verkman and S. Jayaraman-Immunolocalization of CFTR in intact tissue and cultured cells / C.R. Marino-Analysis of CFTR trafficking and polarization using green fluorescent protein and confocal microscopy / B.D. Moyer and B.A. Stanton-CFTR folding and maturation in cells / M. Benharouga, M. Sharma and G.L. Lukacs-Isolation of CFTR. Chaperone complexes by co-immunoprecipitation / G.C. Meacham and D.M. Cyr-CFTR expression and er-associated degradation in yeast / Y. Zhang, S. Michaelis and J.L. Brodsky-Manipulating the folding pathway of delta f508 CFTR using chemical chaperones / M. Howard and W.J. Welch-CFTR degradation and aggregation / M.J. Corboy, P.J. Thomas and W.C. Wigley-In vitro reconstitution of CFTR biogenesis and degradation / J. Oberdorf and W.R. Skach-In vitro CFTR folding assays / R.D. Stidham, W.C. Wigley and P.J. Thomas-Analysis of CFTR endocytosis by cell surface biotinylation / K. Weixel and N.A. Bradbury-CFTR regulation of enac / S.H. Donaldson, E.G. Poligone and M.J. Stutts-Yeast two-hybrid identification and analysis of protein interactions with CFTR / V. Raghuram, K.R. Hallows and J.K. Foskett-Biochemical assays for studying indirect interactions between CFTR and the cytoskeleton / P.J. Mohler [and others]-CFTR-associated atp transport and release / M.E. Egan-Inflammatory mediators in CF patients / J.B. Hilliard, M.W. Konstan and P.B. Davis-Bacterial colonization and infection in the CF lung / S.D. Sagel, E.B. Dowell and F.J. Accurso-Antimicrobial peptides and proteins in the CF airway / A.M. Cole and T. Ganz-Bacterial-epithelial interactions / R. Bryan and A. Prince-Thin-film measurements of airway surface liquid volume/composition and mucus transport rates in vitro / R. Tarran and R.C. Boucher-Murine models of CF airway infection and inflammation / J.F. Chmiel, M.W. Konstan and M. Berger-Analysis of lipid abnormalities in CF mice / S.D. Freedman [and others]-Bioelectric measurement of CFTR function in mice / B.R. Grubb-Xenograft model of the CF airway / M. Filali [and others]-Development of conditionally immortalized epithelial cell lines from CF and non-CF mice / C.U. Cotton-Technical approaches to analyze the in vivo ion composition of airway surface liquid / J.M. Zahm [and others]-Design of gene therapy clinical trials in CF patients / K.V. Curlee and E.J. Sorscher-Formulation of synthetic vectors for cystic fibrosis gene therapy / J. Marshall and S.H. Cheng-Adeno-associated viral vectors for CF gene therapy / T.R. Flotte, I. Virella-Lowell and K.A. Chesnut |
| Summary |
Since the cloning of the cystic fibrosis transmembrane conductance regulator (CFTR) nearly a decade ago, cystic fibrosis researchers, clinicians, and patients have come to rely increasingly on a diverse array of fundamental techniques to understand the molecular basis of this complex disease. Cystic Fibrosis Methods and Protocols consolidates a broad range of detailed and readily reproducible in vitro, cellular, and whole animal laboratory protocols into an indispensable resource. From electrophysiology and cell biology, to animal models and gene therapy, this comprehensive set of methods provides the step-by-step instructions needed for investigators to incorporate new approaches into their research programs. Specific protocols describe new techniques for diagnosis, in vitro methods for the expression and functional analysis of CFTR, novel biochemical and cellular systems to determine how mutations subvert CFTR function, and in vivo protocols to examine how CFTR dysfunction produces multisystem pathology in human and animal models. Comprehensive, multidisciplinary, and highly practical, Cystic Fibrosis Methods and Protocols makes accessible to today's cystic fibrosis investigator the powerful new scientific techniques required to investigate the basic science of the disease and to translate this into effective clinical solutions |
| Bibliography |
Includes bibliographical references and index |
| Notes |
CFTR mutation detection by multiplex heteroduplex (mhet) analysis on mde gel / J. Zielenski [and others]-Cdna microarrays for pharmacogenomic analysis of cystic fibrosis / M. Srivastava, O. Eidelman and H.B. Pollard-Natural animal models of human genetic diseases / J.J. Wine, M. Dean and D. Glavac-Electrophysiological approach to studying CFTR / H. Fischer-Quantitative analysis of atp-dependent gating of CFTR / A. Powe [and others]-CFTR regulation by phosphorylation / T. Zhu [and others]-Transepithelial measurements of bicarbonate secretion in calu-3 cells / R.J. Bridges-Transepithelial impedance analysis of chloride secretion / A.K. Singh [and others]-Studies of the molecular basis for cystic fibrosis using purified reconstituted CFTR protein / I. Kogan [and others]-Probing CFTR channel structure and function using the substituted-cysteine-accessibility method / M.H. Akabas-Methods for the study of intermolecular and intramolecular interactions regulating CFTR function / A.P. Naren-Fluorescent indicator methods to assay functional CFTR expression in cells / A.S. Verkman and S. Jayaraman-Immunolocalization of CFTR in intact tissue and cultured cells / C.R. Marino-Analysis of CFTR trafficking and polarization using green fluorescent protein and confocal microscopy / B.D. Moyer and B.A. Stanton-CFTR folding and maturation in cells / M. Benharouga, M. Sharma and G.L. Lukacs-Isolation of CFTR. Chaperone complexes by co-immunoprecipitation / G.C. Meacham and D.M. Cyr-CFTR expression and er-associated degradation in yeast / Y. Zhang, S. Michaelis and J.L. Brodsky-Manipulating the folding pathway of delta f508 CFTR using chemical chaperones / M. Howard and W.J. Welch-CFTR degradation and aggregation / M.J. Corboy, P.J. Thomas and W.C. Wigley-In vitro reconstitution of CFTR biogenesis and degradation / J. Oberdorf and W.R. Skach-In vitro CFTR folding assays / R.D. Stidham, W.C. Wigley and P.J. Thomas-Analysis of CFTR endocytosis by cell surface biotinylation / K. Weixel and N.A. Bradbury-CFTR regulation of enac / S.H. Donaldson, E.G. Poligone and M.J. Stutts-Yeast two-hybrid identification and analysis of protein interactions with CFTR / V. Raghuram, K.R. Hallows and J.K. Foskett-Biochemical assays for studying indirect interactions between CFTR and the cytoskeleton / P.J. Mohler [and others]-CFTR-associated atp transport and release / M.E. Egan-Inflammatory mediators in CF patients / J.B. Hilliard, M.W. Konstan and P.B. Davis-Bacterial colonization and infection in the CF lung / S.D. Sagel, E.B. Dowell and F.J. Accurso-Antimicrobial peptides and proteins in the CF airway / A.M. Cole and T. Ganz-Bacterial-epithelial interactions / R. Bryan and A. Prince-Thin-film measurements of airway surface liquid volume/composition and mucus transport rates in vitro / R. Tarran and R.C. Boucher-Murine models of CF airway infection and inflammation / J.F. Chmiel, M.W. Konstan and M. Berger-Analysis of lipid abnormalities in CF mice / S.D. Freedman [and others]-Bioelectric measurement of CFTR function in mice / B.R. Grubb-Xenograft model of the CF airway / M. Filali [and others]-Development of conditionally immortalized epithelial cell lines from CF and non-CF mice / C.U. Cotton-Technical approaches to analyze the in vivo ion composition of airway surface liquid / J.M. Zahm [and others]-Design of gene therapy clinical trials in CF patients / K.V. Curlee and E.J. Sorscher-Formulation of synthetic vectors for cystic fibrosis gene therapy / J. Marshall and S.H. Cheng-Adeno-associated viral vectors for CF gene therapy / T.R. Flotte, I. Virella-Lowell and K.A. Chesnut |
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Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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English |
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Print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| In |
Springer Protocols |
| Subject |
Cystic fibrosis -- Laboratory manuals
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Cystic Fibrosis Transmembrane Conductance Regulator
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Cystic Fibrosis -- genetics
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MEDICAL -- Gastroenterology.
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Cystic fibrosis
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Kystische fibrose.
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Wetenschappelijke technieken.
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| Genre/Form |
Handbooks and manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Skach, William
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| ISBN |
9781592591879 |
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1592591876 |
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0896038971 |
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9780896038974 |
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1280821493 |
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9781280821493 |
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9786610821495 |
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6610821496 |
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