| Description |
1 online resource (320 p.) |
| Contents |
Front Cover -- TRYPTAMINE MICROBIOTA-DEREGULATED AMINOACYL-TRNA BIOSYNTHESIS -- TRYPTAMINE MICROBIOTA-DEREGULATED AMINOACYL-TRNA BIOSYNTHESIS -- Contents -- About the author -- Preface -- References -- Chapter 1 -- Introduction -- 1. Modeling cell death and neurodegenerative diseases with human gut metabolite tryptamine inhibiting tryptophanyl-tRNA synthe ... -- 1.1 Our achievements and the book main focus -- 1.2 Tryptamine and related molecules: Contents, origins, cytotoxicity, serotonin transporter, antidepressants -- 1.2.1 Conclusions -- 1.3 Tryptamine in brain -- 1.3.1 Conclusions |
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1.4 Factors affecting tryptamine content in humans -- 2. Pollen microbiota and tryptamine in pollen and honey -- 3. Tryptamine-related pathways and diseases -- 4. ADAS in diseases -- 5. Bidens pilosa, obesity, amyotrophic lateral sclerosis, and tryptamine -- 6. Aminoacyl-tRNA biosynthesis pathway deregulation in different diseases -- 7. Conceptual and methodological pitfalls -- 7.1 Conceptual pitfalls -- 7.2 Methodological pitfalls in the analysis of protein levels, enzymatic activities, and metabolites in diseases -- References |
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Chapter 2 -- Human gut microbial metabolite tryptamine inducing neurodegeneration causes chromosome and genome instability -- 1. Introduction -- 2. Experimental procedures -- 2.1 Cell cultivation -- 2.2 Analysis of chromosomes from MDBK cells and tryptamine-treated or tryptophanol-treated MDBK sublines -- 2.3 Enzymatic activity in cell extracts -- 2.4 DNA isolation for analysis with restriction enzyme EcoR1 -- 2.5 DNA hybridization -- 2.6 Labeling of cells for chase analysis and preparation of postmitochondrial cell extracts for immunoprecipitation: Human TrpR .. |
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2.7 Visualization of tangle of twisted filaments by immune electron microscopy -- 3. Experimental data -- 3.1 Chromosomal Robertsonian translocations in MDBK cells -- 3.2 Chromosomal rearrangements in Djungarian hamster SV40 transformed fibroblastic cell line DM-15 following tryptamine treatment -- 3.3 Tetraploidization in tryptamine- and tryptophanol-treated MDBK cells -- 3.4 Tryptamine induces DNA fragmentation and WARS gene perturbations -- 3.5 Tryptamine alters TrpRS protein level, half-life, and enzymatic activity -- 3.5.1 MDBK cells -- 3.5.2 HeLa cells -- 3.5.3 Human neuronal cells |
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3.6 Cytoplasmic TrpRS gene WARS expression in human cancer cells with different numbers of chromosome 14 possessing WARS -- 3.7 Immunohistochemistry and electron microscopy of MDBK cell treated with tryptamine or tryptophanol -- 4. Discussion -- 4.1 Chromosomal duplications and Robertsonian translocations in human pathologies -- 4.2 Tetraploidization -- 4.3 Tryptamine as DNA intercalator and inducer of DNA perturbations and genomic instability -- 4.4 Chromosomal and genomic changes in Alzheimer's disease -- 4.5 Tryptamine alters transcription of different genes |
| Notes |
Description based upon print version of record |
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4.6 Possible role of tryptamine as the etiological factor and marker of disorders caused by genetic mosaicism |
| Form |
Electronic book
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| ISBN |
9780443288319 |
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0443288313 |
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