Title Spectroscopic methods of analysis : methods and protocols / edited by Wlodek M. Bujalowski

Published New York : Humana Press, ©2012

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Description 1 online resource (xii, 397 pages) Series Methods in molecular biology, 1064-3745 ; 875 Methods in molecular biology (Clifton, N.J.) ; 875 Contents Fluorescence lifetime imaging comes of age how to do it and how to interpret it / Yi-Chun Chen, Bryan Q. Spring, and Robert M. Clegg -- Förster resonance energy transfer and trapping in selected systems : analysis by Monte-Carlo simulation / P. Bojarski [and others] -- Molecular organization of polyene antibiotic amphotericin B studied by means of fluorescence technique / Wieslaw I. Gruszecki [and others] -- Spectroscopic probes of RNA structure and dynamics / Kathleen B. Hall -- Fluorescence methods to study DNA translocation and unwinding kinetics by nucleic acid motors / Christopher J. Fischer [and others] -- Fluorescence intensity, anisotropy, and transient dynamic quenching stopped-flow kinetics / Wlodek M. Bujalowski and Maria J. Jezewska -- Using structure-function constraints in FRET studies of large macromolecular complexes / Wlodek M. Bujalowski and Maria J. Jezewska -- Illuminating allostery in metal sensing transcriptional regulators / Nicholas E. Grossoehme and David P. Giedroc -- Fluorescence-based biosensors / Maria Strianese [and others] -- Metal-enhanced immunoassays / Ignacy Gryczynski [and others] -- Initial stages of angiosperm greening monitored by low-temperature fluorescence spectra and fluorescence lifetimes / Beata Mysliwa-Kurdziel, Anna Stecka, and Kazimierz Strzalka -- Activation of the mammalian cells by using light-sensitive ion channels / Mandy Siu Yu Lung, Paul Pilowsky, and Ewa M. Goldys -- Detection of specific strains of viable bacterial pathogens by using RNA bead assays and flow cytometry with 2100 Bioanalyzer / Philip Butterworth [and others] -- Photosynthetic antenna complex LHCII studied with novel fluorescence techniques / Wieslaw I. Gruszecki [and others] -- Analysis of RNA folding and ribonucleoprotein assembly by single-molecule fluorescence spectroscopy / Goran Pljevaljčić [and others] -- Single-molecule force spectroscopy of polycystic kidney disease proteins / Liang Ma, Meixiang Xu, and Andres F. Oberhauser -- Single molecule detection approach to muscle study : kinetics of a single cross-bridge during contraction of muscle / Julian Borejdo [and others] -- Single-molecule optical-trapping measurements with DNA anchored to an array of gold nanoposts / D. Hern Paik and Thomas T. Perkins -- Electro-optical analysis of macromolecular structure and dynamics / Dietmar Porschke -- Cryoradiolysis and cryospectroscopy for studies of heme-oxygen intermediates in cytochromes P450 / I.G. Denisov, Y.V. Grinkova, and S.G. Sligar Summary Quantitative elucidation of structural, energetic and dynamic aspects of macromolecular interactions is indispensable for understanding the functional activities of biomolecules and their interactions. The optical spectroscopic methods are not confined to small molecules or macromolecules but permit the studies of even the largest biological systems in their full splendor, including the living cell. In, Spectroscopic Methods of Analysis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study properties of individual macromolecules, their complexes, organelles, and cells, using optical spectroscopic techniques. These include methods and approaches for experimental and theoretical analyses of fluorescence properties of the examined systems, single molecule approaches, electronic absorption, and electro-optical analyses of macromolecular interactions, structures, and dynamics. Written in the highly successful Methods in Molecular Biology(tm) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Spectroscopic Methods of Analysis : Methods and Protocols seeks to aid scientists in the further study of optical spectroscopic methods Analysis Medicine Radiology, Medical Cytology -- Research -- Methodology Medicine & Public Health Imaging / Radiology Biological Techniques Cell Biology geneeskunde volksgezondheid public health afbeelden imagery radiologie radiology celbiologie cellular biology biologische technieken Medicine (General) Geneeskunde (algemeen) Bibliography Includes bibliographical references and index Notes English Subject Optical spectroscopy -- Laboratory manuals Fluorimetry. Photometry. Fluorescence spectroscopy. Spectrophotometry. Chemistry Techniques, Analytical Fluorometry Photometry Luminescent Measurements Investigative Techniques Analytical, Diagnostic and Therapeutic Techniques and Equipment Spectrometry, Fluorescence Spectrophotometry Spectrum Analysis spectrophotometry. Spectrophotometry Photometry Fluorimetry Fluorescence spectroscopy Optical spectroscopy Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Bujalowski, Wlodek M ISBN 9781617798061 1617798061 1617798053 9781617798054

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