Title Intracellular pH and its measurement / authors, Arnošt Kotyk and Jan Slavík

Published Boca Raton, Fla. : CRC Press, ©1989

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Description 1 online resource (182 pages : illustrations Contents Cover -- Half Title -- Title Page -- Copyright Page -- Preface -- The Authors -- Table of Contents -- Chapter 1: The Concept of pH, Hydrolysis, and Buffers -- Chapter 2: pH in Biological Systems -- Chapter 3: Methods of Determining Intracellular pH with Electrodes -- Chapter 4A: Assays of Intracellular pH Using Chemical Probes: Principles of pH Indicator Response -- Chapter 4B: Assays of Intracellular pH Using Chemical Probes: Distribution Techniques -- Chapter 4C: Assays of Intracellular pH Using Chemical Probes: Absorption Spectroscopy Chapter 4D: Assays of Intracellular pH Using Chemical Probes: Fluorescence Spectroscopy -- Chapter 4E: Assays of Intracellular pH Using Chemical Probes: Nuclear Magnetic Resonance Spectroscopy -- Chapter 5: The Choice of the Assay Method -- Chapter 6: Heterogeneity of Intracellular pH -- Index Summary This volume introduces a summary of all the techniques used to estimate pH reliably. Emphasis is placed on the techniques that provide the most reliable and detailed data. The role of cell pH is explained with special emphasis on enzymology and membrane transport and bioenergetics. This book was written especially for molecular biologists, biochemists and biophysicists Bibliography Includes bibliographical references and index Notes Arnost Kotyk and Jan Slavik Subject Cytochemistry -- Methodology Hydrogen-ion concentration -- Measurement. SCIENCE -- Life Sciences -- General. Cytochemistry -- Methodology Hydrogen-ion concentration -- Measurement Form Electronic book Author Slavík, Jan, 1951-1999. LC no. 88019253 ISBN 9781000102437 1000102432 9781003068266 100306826X 9781000141627 1000141624 9781000120295 1000120295

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