Title Electron microscopy methods and protocols / edited by M.A. Nasser Hajibagheri

Published Totowa, N.J. : Humana Press, ©1999

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Description 1 online resource (xi, 283 pages, 4 pages of plates) : illustrations (some color) Series Methods in molecular biology ; v. 117 Methods in molecular biology (Clifton, N.J.) ; v. 117 Contents General Preparation of Material and Staining of Sections / Heather A. Davies -- Negative Staining of Thinly Spread Biological Particulates / J. Robin Harris -- Preparation of Thin-Film Frozen-Hydrated/ Vitrified Biological Specimens for Cryoelectron Microscopy / J. Robin Harris and Marc Adrian -- The Production of Cryosections through Fixed and Cryoprotected Biological Material and Their Use in Immunocytochemistry / Paul Webster -- High-Pressure Freezing for Preservation of High Resolution Fine Structure and Antigenicity for Immunolabelling / Kent McDonald -- The Application of LR Gold Resin for Immunogold Labeling / J.R. Thorpe -- Low-Temperature Embedding in Acrylic Resins / Pierre Gounon -- Quantitative Aspects of Immunogold Labeling in Embedded and Nonembedded Sections / Catherine Rabouille -- Microwave Processing Techniques for Electron Microscopy: A Four-Hour Protocol / Rick T. Giberson and Richard S. Demaree, Jr. -- Electron Microscopic Enzyme Cytochemistry / Nobukazu Araki and Tanenori Hatae -- In Situ Molecular Hybridization Techniques for Ultrathin Sections / Jean-Guy Fournier and Francoise Escaig-Haye -- Preparation of the Fission Yeast Schizosaccharomyces pombe for Ultrastructural and Immunocytochemical Study / M.A. Nasser Hajibagheri [and others] -- Preparation of Double/Single-Stranded DNA and RNA Molecules for Electron Microscopy / M.A. Nasser Hajibagheri -- Applications of Electron Microscopy for Studying Protein-DNA Complexes / Maria Schnos and Ross B. Inman -- X-Ray Microanalysis Techniques / A. John Morgan, Carole Winters, and Stephen Sturzenbaum Summary In Electron Microscopy Methods and Protocols, well-practiced experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological X-ray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryo-fixed or chemically-fixed cells are described in sufficient detail to provide practical insight into their advantages and limitations. Electron Microscopy Methods and Protocols offers both newcomers and established researchers wanting to expand their repertoire of cutting-edge electron microscopy techniques-each optimized for reproducibility and robust results-today's gold-standard laboratory manual Analysis elektronenmicroscopie electron microscopy methodologie methodology biologische technieken biological techniques microscopie microscopy kleuring staining protocollen protocols Biological Techniques Biologische technieken Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Print version record Subject Electron microscopy -- Laboratory manuals Histology -- Technique. Electron microscopy. Microscopy, Electron Histocytological Preparation Techniques Histological Techniques electron microscopy. NATURE -- Reference. SCIENCE -- Life Sciences -- General. SCIENCE -- Life Sciences -- Biology. Electron microscopy Histology -- Technique Microscopie électronique -- Méthode. Elektronenmicroscopie. Wetenschappelijke technieken. Histologie. Microscopie électronique -- Technique. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Hajibagheri, M. A. Nasser ISBN 0585244006 9780585244006 9781592592012 1592592015 0896036405 9780896036406

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