| Description |
1 online resource (129 p.) |
| Contents |
Intro -- Half Title -- Title Page -- Copyright Page -- Contents -- Preface -- Acknowledgments -- Editor Biographies -- Contributors -- Foreword A and B -- Foreword A -- Foreword B -- Section 1: Basics in Cell Culture -- 1. Highlights: History of Cell Culture and Its Applications -- 1.1 Milestones in Cell Culture Techniques -- References -- 2. Aseptic Technique and Essentials in Cell Culture -- 2.1 Aseptic Technique -- 2.2 Materials -- 2.3 Procedure -- 2.3.1 Cell Line Background -- 2.3.2 Essential Information, Including Cell Numbers -- References -- 3. Laboratory Safety |
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3.1 Key Issues in Cell Culture Laboratory -- 3.1.1 Biosafety Level Containment and Biohazards -- 3.1.2 Acute Toxicity from Accidental Exposure to Toxic Chemicals, Gases, and Liquids, and Physical Injury -- 3.1.3 Treatment and Disposal of Cell Culture Waste -- 3.1.4 Dangers in Liquid Nitrogen Handling in the Lab -- References -- 4. Preparation of Media for Cell Culture -- 4.1 Introduction -- 4.2 Materials -- 4.3 Methodology -- 4.4 Key Points -- References -- 5. Primary Cell Culture -- 5.1 Introduction -- 5.2 Materials -- 5.3 Methodology -- 5.4 Key Points -- References -- 6. Passaging of Cell Lines |
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6.1 Introduction -- 6.2 Passaging of Adherent Cell Lines -- 6.2.1 Materials -- 6.2.2 Methodology -- 6.2.3 Key Points -- 6.3 Passaging of Suspension Cell Lines -- 6.3.1 Materials -- 6.3.2 Methodology -- 6.3.3 Key Points -- References -- 7. Cell or Tissue Culture Models and Cell Line Immortalization -- 7.1 Basic Concepts -- References -- 8. Counting of Cells by Hemocytometer -- 8.1 Introduction -- 8.2 Materials -- 8.3 Methodology -- 8.4 Key Points -- References -- 9. Cryopreservation of Cells -- 9.1 Introduction -- 9.2 Guidelines for Cryopreservation -- 9.2.1 Serum-Containing Medium Constituents |
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9.2.2 Some of the Common Media Constituents for Serum-Free Medium -- 9.3 Materials -- 9.4 Methodology -- 9.4.1 Methodology: Suspension Cultures -- 9.4.2 Methodology: Adherent Cultures -- References -- 10. Thawing of Cells -- 10.1 Introduction -- 10.2 Guidelines for Thawing Cells -- 10.3 Materials -- 10.4 Methodology -- References -- 11. Detection of Bacterial and Fungal Contamination -- 11.1 Introduction -- 11.2 Materials -- 11.3 Methodology -- 11.4 Key Point -- References -- 12. Testing for Mycoplasma Contamination -- 12.1 Introduction -- 12.2 Materials -- 12.3 Methodology -- 12.4 Key Points |
| Notes |
Description based upon print version of record |
| Bibliography |
References -- 13. Cellular Cytotoxicity -- 13.1 MTT Assay -- 13.1.1 Introduction -- 13.1.2 Materials -- 13.1.3 Methodology -- 13.1.4 Key Points -- 13.2 Alamar Blue Assay -- 13.2.1 Introduction -- 13.2.2 Materials -- 13.2.3 Methodology -- 13.2.4 Key Points -- References -- Section 2: Isolation and Analysis of Nucleic Acids and Proteins -- 14. Isolation of Total RNA and Assessment of Purity and Concentration -- 14.1 Introduction -- 14.2 Materials -- 14.3 Methodology -- 14.4 Key Points -- 14.5 Quibt 3.0 Analysis -- 14.5.1 Introduction -- 14.5.2 Materials -- 14.5.3 Methodology -- 14.6 Key Points |
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References |
| Form |
Electronic book
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| Author |
Senthilkumar, R
|
|
Hoque, Enamul
|
| ISBN |
9781000969405 |
|
1000969401 |
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