| Description |
1 online resource (338 pages) |
| Contents |
880-01 GENE CLONING AND DNA ANALYSIS; Contents; TO THE SIXTH EDITION Preface to the Sixth Edition; PART I The Basic Principles of Gene Cloning and DNA Analysis; Chapter 1 Why Gene Cloning and DNA Analysis are Important; Chapter 2 Vectors for Gene Cloning:Plasmids and Bacteriophages; Chapter 3 Purification of DNA from Living Cells; Chapter 4 Manipulation of Purified DNA; Chapter 5 Introduction of DNA into Living Cells; Chapter 6 Cloning Vectors for E.coli; Chapter 7 Cloning Vectors for Eukaryotes; Chapter 8 How to Obtain a Clone of a Specific Gene; Chapter 9 The Polymerase Chain Reaction |
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880-01/(S 5.2.1 Recombinant selection with pBR322 -- insertional inactivation of an antibiotic resistance gene -- 5.2.2 Insertional inactivation does not always involve antibiotic resistance -- 5.3 Introduction of phage DNA into bacterial cells -- 5.3.1 Transfection -- 5.3.2 In vitro packaging of λ cloning vectors -- 5.3.3 Phage infection is visualized as plaques on an agar medium -- 5.4 Identification of recombinant phages -- 5.4.1 Insertional inactivation of a lacZ′ gene carried by the phage vector -- 5.4.2 Insertional inactivation of the λ cI gene -- 5.4.3 Selection using the Spi phenotype -- 5.4.4 Selection on the basis of λ genome size -- 5.5 Introduction of DNA into non-bacterial cells -- 5.5.1 Transformation of individual cells -- 5.5.2 Transformation of whole organisms -- Chapter 6 Cloning Vectors for E.coli -- 6.1 Cloning vectors based on E.coli plasmids -- 6.1.1 The nomenclature of plasmid cloning vectors -- 6.1.2 The useful properties of pBR322 -- 6.1.3 The pedigree of pBR322 -- 6.1.4 More sophisticated E.coli plasmid cloning vectors -- pUC8 -- a Lac selection plasmid -- pGEM3Z -- in vitro transcription of cloned DNA -- 6.2 Cloning vectors based on M13 bacteriophage -- 6.2.1 How to construct a phage cloning vector -- 6.2.2 Hybrid plasmid-M13 vectors -- 6.3 Cloning vectors based on λ bacteriophage -- 6.3.1 Segments of the λ genome can impairing viability -- 6.3.2 Natural selection can be used to isolate modified λ that lack certain restriction sites -- 6.3.3 Insertion and replacement vectors -- Insertion vectors -- Replacement vectors -- 6.3.4 Cloning experiments with λ insertion or replacement vectors -- 6.3.5 Long DNA fragments can be cloned using a cosmid -- 6.4 8 and other high-capacity vectors enable genomic libraries to be constructed -- 6.5 Vectors for other bacteria -- Chapter 7 Cloning Vectors for Eukaryotes |
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PART II The Applications of Gene Cloning and DNA Analysis in ResearchChapter 10 Sequencing Genes and Genomes; Chapter 11 Studying Gene Expression and Function; Chapter 12 Studying Genomes; PART III The Applications of Gene Cloning and DNA Analysis in Biotechnology; Chapter 13 Production of Protein from Cloned Genes; Chapter 14 Gene Cloning and DNA Analysis in Medicine; Chapter 15 Gene Cloning and DNA Analysis in Agriculture; C |
| Summary |
Known world-wide as the standard introductory text to this important and exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly upda |
| Notes |
Print version record |
| Subject |
DNA.
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Molecular cloning.
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Nucleotide sequence.
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DNA
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Cloning, Molecular
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DNA
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Molecular cloning
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Nucleotide sequence
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| Form |
Electronic book
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| ISBN |
9781444318616 |
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1444318616 |
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